SASBDB entries for UniProt ID:

SASDVN2 – S.aureus Protein Map Eap3 and Eap4 domains (Δ266-476)

UniProt ID: Q99QS1 (266-476) Protein map
Protein map experimental SAS data
MULTIFOXS model
Sample: Protein map monomer, 24 kDa Staphylococcus aureus (strain … protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Nov 17
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 28 nm3

SASDB76 – Deglycosylated myelin-associated glycoprotein (immunoglobulin domains 1-3)

UniProt ID: P20917 (20-325) Myelin-associated glycoprotein Ig domains 1-3
Myelin-associated glycoprotein Ig domains 1-3 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myelin-associated glycoprotein Ig domains 1-3 monomer, 35 kDa Mus musculus protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11
Structural basis of myelin-associated glycoprotein adhesion and signalling. Nat Commun 7:13584 (2016)
Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
RgGuinier 3.9 nm
Dmax 12.6 nm
VolumePorod 49 nm3

SASDCQ4 – Collagenase ColH s2as2bs3 at pCa 5

UniProt ID: Q46085 (718-1021) Collagenase ColH segement s2as2bs3
Collagenase ColH segement s2as2bs3 experimental SAS data
DAMMIF model
Sample: Collagenase ColH segement s2as2bs3 monomer, 34 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 12
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.3 nm
Dmax 14.2 nm
VolumePorod 37 nm3

SASDE39 – Interleukin-18 receptor accessory protein ectodomain with Rα linker

UniProt ID: O95256 (20-364) Interleukin-18 receptor accessory protein ectodomain with Rα linker
Interleukin-18 receptor accessory protein ectodomain with Rα linker experimental SAS data
BILBOMD model
Sample: Interleukin-18 receptor accessory protein ectodomain with Rα linker monomer, 41 kDa Homo sapiens protein
Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 24
Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling. Structure 27(8):1296-1307.e5 (2019)
Ge J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X
RgGuinier 3.4 nm
Dmax 11.5 nm
VolumePorod 72 nm3

SASDF46 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 100 mM NaCl

UniProt ID: None (None-None) 80bp_DNA Forward

UniProt ID: None (None-None) 80bp_DNA Reverse

UniProt ID: P0ACF0 (1-90) DNA-binding protein HU-alpha
80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
DNA-binding protein HU-alpha, 10 kDa Escherichia coli protein
Buffer: 10 mM sodium acetate, 100 mM NaCl, pH: 4.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 1
Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDJY9 – Mammalian cell entry protein 4A (Mce4A39-320)

UniProt ID: None (None-None) n-Dodecyl-β-D-Maltopyranoside

UniProt ID: I6YC99 (39-320) Mce-family protein Mce4A
n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data
CUSTOM IN-HOUSE model
Sample: N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Mce-family protein Mce4A monomer, 34 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 2
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
RgGuinier 4.8 nm
Dmax 18.2 nm
VolumePorod 189 nm3

SASDKE7 – Bromodomain-containing protein 4, BRD4, tandem bromodomains (10 mg/ml)

UniProt ID: O60885 (38-460) Bromodomain-containing protein 4
Bromodomain-containing protein 4 experimental SAS data
Bromodomain-containing protein 4 Kratky plot
Sample: Bromodomain-containing protein 4 monomer, 48 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25
Multivalent nucleosome scaffolding by bromodomain and extraterminal domain tandem bromodomains. J Biol Chem :108289 (2025)
Olp MD, Bursch KL, Wynia-Smith SL, Nuñez R, Goetz CJ, Jackson V, Smith BC
RgGuinier 7.3 nm
Dmax 20.2 nm
VolumePorod 150 nm3

SASDLE3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 9 mg/mL + UFM1 2.25 mg/mL)

UniProt ID: Q9GZZ9 (57-346) Ubiquitin-like modifier-activating enzyme 5

UniProt ID: P61960 (1-83) Ubiquitin fold modifer 1
Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data
SASREF model
Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29
Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M
RgGuinier 3.2 nm
Dmax 14.5 nm
VolumePorod 111 nm3

SASDLX3 – Human Albumin (C4)

UniProt ID: P02768 (None-None) Albumin
Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1
Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M
RgGuinier 2.8 nm
Dmax 8.4 nm

SASDL58 – The Fe–S cluster assembly 1 homolog of pigeon before applying the external magnetic field (0 min)

UniProt ID: P0DN75 (2-132) Iron-sulfur cluster assembly 1 homolog, mitochondrial
Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 4.7 nm
Dmax 15.8 nm