SASBDB entries for UniProt ID:

SASDK77 – Bromodomain-containing protein 2, BRD2, tandem bromodomains (10 mg/ml)

UniProt ID: P25440 (71-455) Bromodomain-containing protein 2
Bromodomain-containing protein 2 experimental SAS data
Bromodomain-containing protein 2 Kratky plot
Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25
Multivalent nucleosome scaffolding by bromodomain and extraterminal domain tandem bromodomains. J Biol Chem :108289 (2025)
Olp MD, Bursch KL, Wynia-Smith SL, Nuñez R, Goetz CJ, Jackson V, Smith BC
RgGuinier 5.2 nm
Dmax 18.3 nm
VolumePorod 100 nm3

SASDK38 – Lipid A phosphoethanolamine transferase in n-dodecyl-β-D maltoside micelles

UniProt ID: Q7DD94 (1-544) YhbX/YhjW/YijP/YjdB family protein (L152F)
YhbX/YhjW/YijP/YjdB family protein (L152F) experimental SAS data
Lipid A phosphoethanolamine transferase in n-dodecyl-β-D maltoside micelles Rg histogram
Sample: YhbX/YhjW/YijP/YjdB family protein (L152F) monomer, 62 kDa Neisseria meningitidis serogroup … protein
Buffer: 50 mM HEPES, 100 mM NaCl, 0.023% n-Dodecyl β-D-maltoside (DDM), pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 26
Conformational flexibility of EptA driven by an interdomain helix provides insights for enzyme-substrate recognition. IUCrJ 8(Pt 5):732-746 (2021)
Anandan A, Dunstan NW, Ryan TM, Mertens HDT, Lim KYL, Evans GL, Kahler CM, Vrielink A
RgGuinier 4.2 nm
Dmax 12.9 nm
VolumePorod 304 nm3

SASDKF8 – N-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor

UniProt ID: B7UM99 (1-233) Translocated intimin receptor Tir
Translocated intimin receptor Tir experimental SAS data
Sample: Translocated intimin receptor Tir dimer, 50 kDa Escherichia coli O127:H6 … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 18
The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection. Commun Biol 7(1):179 (2024)
Vieira MFM, Hernandez G, Zhong Q, Arbesú M, Veloso T, Gomes T, Martins ML, Monteiro H, Frazão C, Frankel G, Zanzoni A, Cordeiro TN
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 46 nm3

SASDL32 – Transcription elongation factor SPT6

UniProt ID: P23615 (None-None) Transcription elongation factor SPT6
Transcription elongation factor SPT6 experimental SAS data
Transcription elongation factor SPT6 Rg histogram
Sample: Transcription elongation factor SPT6 monomer, 145 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 22
Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly. Nucleic Acids Res (2022)
Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R
RgGuinier 4.5 nm
Dmax 12.0 nm
VolumePorod 249 nm3

SASDLW2 – Accessory colonization factor SslE at pH 4.4

UniProt ID: E0IW31 (90-1520) Accessory colonization factor
Accessory colonization factor experimental SAS data
DAMMIN model
Sample: Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein
Buffer: 20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 4.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 27
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 248 nm3

SASDL73 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 2 mg/mL + UFM1 0.5 mg/mL)

UniProt ID: Q9GZZ9 (57-346) Ubiquitin-like modifier-activating enzyme 5

UniProt ID: P61960 (1-83) Ubiquitin fold modifer 1
Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data
SASREF model
Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29
Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M
RgGuinier 2.9 nm
Dmax 11.0 nm

SASDLM3 – Interleukin-11 signalling complex, truncated gp130

UniProt ID: P40189 (22-324) Interleukin-6 receptor subunit beta

UniProt ID: P20809 (32-199) Interleukin-11

UniProt ID: Q14626 (23-319) Interleukin-11 receptor subunit alpha
Interleukin-6 receptor subunit betaInterleukin-11Interleukin-11 receptor subunit alpha experimental SAS data
OTHER model
Sample: Interleukin-6 receptor subunit beta dimer, 69 kDa Homo sapiens protein
Interleukin-11 dimer, 36 kDa Homo sapiens protein
Interleukin-11 receptor subunit alpha dimer, 64 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 5.3 nm
Dmax 17.6 nm
VolumePorod 405 nm3

SASDLC4 – Human Albumin (HSA) Healthy donor used for HNA1, HNA2, HMA fractions

UniProt ID: P02768 (None-None) Albumin
Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1
Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M
RgGuinier 2.8 nm
Dmax 8.5 nm

SASDLR4 – 3-oxoacyl-[acyl-carrier-protein] reductase, FabG

UniProt ID: P0AEK2 (1-244) 3-oxoacyl-[acyl-carrier-protein] reductase FabG
3-oxoacyl-[acyl-carrier-protein] reductase FabG experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 3-oxoacyl-[acyl-carrier-protein] reductase FabG, 26 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1
Protein quaternary structures in solution are a mixture of multiple forms Chemical Science 13(39):11680-11695 (2022)
Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G
RgGuinier 3.6 nm
Dmax 10.6 nm
VolumePorod 212 nm3

SASDL25 – Bacillus subtilis complex of diadenylate cyclase (CdaA:cytoplasmic domain) and phosphoglucosamine mutase (GlmM)

UniProt ID: Q45589 (97-273) Cyclic di-AMP synthase CdaA

UniProt ID: O34824 (1-448) Phosphoglucosamine mutase
Cyclic di-AMP synthase CdaAPhosphoglucosamine mutase experimental SAS data
Cyclic di-AMP synthase CdaA Phosphoglucosamine mutase Kratky plot
Sample: Cyclic di-AMP synthase CdaA dimer, 44 kDa Bacillus subtilis (strain … protein
Phosphoglucosamine mutase dimer, 101 kDa Bacillus subtilis (strain … protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 21
Structural basis for the inhibition of the Bacillus subtilis c-di-AMP cyclase CdaA by the phosphoglucomutase GlmM Journal of Biological Chemistry :101317 (2021)
Pathania M, Tosi T, Millership C, Hoshiga F, Morgan R, Freemont P, Gründling A
RgGuinier 4.5 nm
Dmax 16.2 nm
VolumePorod 250 nm3