SASBDB entries for UniProt ID:

SASDDE6 – Photoreceptor sensory box 1 light-state (SB1-LOV-R66I)

UniProt ID: Q88E39 (None-None) Sensory box protein light-state (R66I)
Sensory box protein light-state (R66I) experimental SAS data
Photoreceptor sensory box 1 light-state (SB1-LOV-R66I) Rg histogram
Sample: Sensory box protein light-state (R66I) dimer, 37 kDa Pseudomonas putida protein
Buffer: 10mM Tris, 10 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 2
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein. PLoS One 13(7):e0200746 (2018)
Röllen K, Granzin J, Batra-Safferling R, Stadler AM
RgGuinier 2.6 nm
Dmax 9.2 nm
VolumePorod 58 nm3

SASDDF6 – Myelin basic protein

UniProt ID: P02687 (None-None) Myelin basic protein
Myelin basic protein experimental SAS data
Myelin basic protein Rg histogram
Sample: Myelin basic protein monomer, 18 kDa Bos taurus protein
Buffer: 20 mM NaH2PO4/ Na2HPO4, 99.9% D2O, pH: 4.8
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 21
Internal nanosecond dynamics in the intrinsically disordered myelin basic protein. J Am Chem Soc 136(19):6987-94 (2014)
Stadler AM, Stingaciu L, Radulescu A, Holderer O, Monkenbusch M, Biehl R, Richter D
RgGuinier 3.3 nm
Dmax 11.1 nm

SASDDG6 – Photoreceptor sensory box 1 dark-state (SB1-LOV-R66I)

UniProt ID: Q88E39 (None-None) Sensory box protein dark-state
Sensory box protein dark-state experimental SAS data
Photoreceptor sensory box 1 dark-state (SB1-LOV-R66I) Rg histogram
Sample: Sensory box protein dark-state dimer, 37 kDa Pseudomonas putida protein
Buffer: 10mM Tris, 10 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 2
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein. PLoS One 13(7):e0200746 (2018)
Röllen K, Granzin J, Batra-Safferling R, Stadler AM
RgGuinier 2.6 nm
Dmax 8.0 nm
VolumePorod 57 nm3

SASDDH6 – Di-domain acyl carrier protein of the seryltransferase from prodigiosin biosynthesis

UniProt ID: Q5W264 (4-191) Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis
Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis experimental SAS data
DAMMIF model
Sample: Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis monomer, 22 kDa Serratia sp. ATCC … protein
Buffer: 20 mM Tris supplemented with 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2016 Jan 13
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.4 nm
Dmax 8.4 nm
VolumePorod 39 nm3

SASDDJ6 – SAM-dependent O-Methyltransferase from Mycobacterium hassiacum

UniProt ID: K5B7F3 (1-219) Methyltransferase domain protein
Methyltransferase domain protein experimental SAS data
MODELLER model
Sample: Methyltransferase domain protein dimer, 51 kDa Mycolicibacterium hassiacum protein
Buffer: 20 mM bis-tris propane, 50 mM NaCl, 2 mM DTT, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jun 21
Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides. Proc Natl Acad Sci U S A 116(3):835-844 (2019)
Ripoll-Rozada J, Costa M, Manso JA, Maranha A, Miranda V, Sequeira A, Ventura MR, Macedo-Ribeiro S, Pereira PJB, Empadinhas N
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 86 nm3

SASDDL6 – Calcium Loaded Calbindin-D28K

UniProt ID: P05937 (None-None) Calbindin
Calbindin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Calbindin monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 3 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 28
The X-ray structure of human calbindin-D28K: an improved model. Acta Crystallogr D Struct Biol 74(Pt 10):1008-1014 (2018)
Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR
RgGuinier 2.1 nm
Dmax 7.3 nm
VolumePorod 47 nm3

SASDDM6 – Calbindin-D28K

UniProt ID: P05937 (None-None) Calbindin
Calbindin experimental SAS data
DAMMIF model
Sample: Calbindin monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150mM NaCl, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 28
The X-ray structure of human calbindin-D28K: an improved model. Acta Crystallogr D Struct Biol 74(Pt 10):1008-1014 (2018)
Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 46 nm3

SASDDN6 – Human stress-inducible heat shock 70 kDa protein 1, Hsp70 (Hspa1a) T204A mutant dimer, in the presence of ATP

UniProt ID: P0DMV8 (None-None) Heat shock 70 kDa protein 1
Heat shock 70 kDa protein 1 experimental SAS data
MODELLER model
Sample: Heat shock 70 kDa protein 1 dimer, 147 kDa Homo sapiens protein
Buffer: 50mM HEPES, 150mM KCH3COO, 2mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2018 Feb 20
Human stress-inducible Hsp70 has a high propensity to form ATP-dependent antiparallel dimers that are differentially regulated by co-chaperone binding. Mol Cell Proteomics (2018)
Trcka F, Durech M, Vankova P, Chmelik J, Martinkova V, Hausner J, Kadek A, Marcoux J, Klumpler T, Vojtesek B, Muller P, Man P
RgGuinier 4.0 nm
Dmax 11.6 nm
VolumePorod 248 nm3

SASDDQ6 – The ferredoxin protease, FusC

UniProt ID: Q6D8U3 (26-924) Ferredoxin Protease
Ferredoxin Protease experimental SAS data
Ferredoxin Protease Kratky plot
Sample: Ferredoxin Protease monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 6
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 12.8 nm
VolumePorod 152 nm3

SASDDR6 – The ferredoxin protease, FusC, E83A mutant

UniProt ID: Q6D8U3 (26-924) Ferredoxin protease E83A mutant
Ferredoxin protease E83A mutant experimental SAS data
Ferredoxin protease E83A mutant Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 6
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 12.7 nm
VolumePorod 152 nm3