Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDFA6 – Proteolytic fragment of phage flagella binding tail protein YSD1_29 (amino acids 373-1296)

UniProt ID: M9NVD3 (373-1296) Flagella binding tail protein

Flagella binding tail protein experimental SAS data

Sample:	Flagella binding tail protein monomer, 103 kDa Salmonella virus Chi protein
Buffer:	20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 4

The flagellotropic bacteriophage YSD1 targets Salmonella Typhi with a Chi-like protein tail fibre. Mol Microbiol (2019)
Dunstan RA, Pickard D, Dougan S, Goulding D, Cormie C, Hardy J, Li F, Grinter R, Harcourt K, Yu L, Song J, Schreiber F, Choudhary J, Clare S, Coulibaly F, Strugnell RA, Dougan G, Lithgow T

R_g^Guinier	5.6	nm
D_max	27.4	nm
Volume^Porod	155	nm³

SASDFB6 – The periplasmically localised protease PqqL from Escherichia coli

UniProt ID: P31828 (27-931) Zinc protease PqqL

Zinc protease PqqL experimental SAS data

Sample:	Zinc protease PqqL monomer, 102 kDa Escherichia coli protein
Buffer:	20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jun 14

Protease-associated import systems are widespread in Gram-negative bacteria. PLoS Genet 15(10):e1008435 (2019)
Grinter R, Leung PM, Wijeyewickrema LC, Littler D, Beckham S, Pike RN, Walker D, Greening C, Lithgow T

R_g^Guinier	4.0	nm
D_max	13.7	nm
Volume^Porod	207	nm³

SASDFC6 – Wild type protein kinase YopO

UniProt ID: Q93KQ6 (89-729) Protein kinase YopO

Protein kinase YopO experimental SAS data

Sample:	Protein kinase YopO monomer, 63 kDa Yersinia enterocolitica protein
Buffer:	10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 6

Studying Conformational Changes of the Yersinia Type-III-Secretion Effector YopO in Solution by Integrative Structural Biology. Structure 27(9):1416-1426.e3 (2019)
Peter MF, Tuukkanen AT, Heubach CA, Selsam A, Duthie FG, Svergun DI, Schiemann O, Hagelueken G

R_g^Guinier	3.3	nm
D_max	11.5	nm
Volume^Porod	119	nm³

SASDFD6 – Protein kinase YopO - cytoplasmic 1 actin complex

UniProt ID: Q93KQ6 (89-729) Protein kinase YopO

UniProt ID: P60709 (1-375) Actin, cytoplasmic 1

Protein kinase YopOActin, cytoplasmic 1 experimental SAS data

Sample:	Protein kinase YopO monomer, 63 kDa Yersinia enterocolitica protein Actin, cytoplasmic 1 monomer, 42 kDa Homo sapiens protein
Buffer:	10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 6

R_g^Guinier	3.6	nm
D_max	12.3	nm
Volume^Porod	147	nm³

SASDFE6 – Sliding beta clamp

UniProt ID: P9WNU1 (1-402) Beta sliding clamp

Beta sliding clamp experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 15

Regulation of futile ligation during early steps of BER in M. tuberculosis is carried out by a β-clamp-XthA-LigA tri-component complex International Journal of Biological Macromolecules (2022)
Shukla A, Afsar M, Khanam T, Kumar N, Ali F, Kumar S, Jahan F, Ramachandran R

R_g^Guinier	3.7	nm
D_max	10.3	nm
Volume^Porod	186	nm³

SASDFF6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp complex

UniProt ID: P9WNU1 (1-402) Beta sliding clamp

UniProt ID: P96273 (1-291) Probable exodeoxyribonuclease III protein XthA (Exonuclease III) (EXO III) (AP endonuclease VI)

Beta sliding clampProbable exodeoxyribonuclease III protein XthA (Exonuclease III) (EXO III) (AP endonuclease VI) experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA (Exonuclease III) (EXO III) (AP endonuclease VI) monomer, 33 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 15

R_g^Guinier	3.5	nm
D_max	11.3	nm
Volume^Porod	98	nm³

SASDFG6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex

UniProt ID: P9WNU1 (1-402) Beta sliding clamp

UniProt ID: A0A0T9L251 (1-289) Probable exodeoxyribonuclease III protein XthA

UniProt ID: P9WNV1 (1-691) DNA ligase A

Beta sliding clampProbable exodeoxyribonuclease III protein XthADNA ligase A experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 31 kDa Mycobacterium tuberculosis protein DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 17

R_g^Guinier	5.8	nm
D_max	25.3	nm
Volume^Porod	501	nm³

SASDFH6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate

UniProt ID: A0A0T9L251 (1-289) Probable exodeoxyribonuclease III protein XthA

UniProt ID: P9WNV1 (None-None) DNA ligase A

UniProt ID: P9WNU1 (1-402) Beta sliding clamp

UniProt ID: None (None-None) DNA ligase A nicked DNA substrate

Probable exodeoxyribonuclease III protein XthADNA ligase ABeta sliding clampDNA ligase A nicked DNA substrate experimental SAS data

Sample:	Probable exodeoxyribonuclease III protein XthA monomer, 31 kDa Mycobacterium tuberculosis protein DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein DNA ligase A nicked DNA substrate dimer, 16 kDa DNA
Buffer:	50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 17

R_g^Guinier	5.8	nm
D_max	19.1	nm
Volume^Porod	496	nm³

SASDFL6 – Adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8

UniProt ID: O25315 (1-598) Adenine specific DNA methyltransferase (Mod)

Adenine specific DNA methyltransferase (Mod) experimental SAS data

Sample:	Adenine specific DNA methyltransferase (Mod) dimer, 137 kDa Helicobacter pylori protein
Buffer:	25 mM Tris, 250 mM NaCl, pH: 8
Experiment:	SAXS data collected at ID14-3, ESRF on 2017 Jul 9

Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress. J Mol Biol (2019)
Narayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT

R_g^Guinier	3.3	nm
D_max	12.5	nm
Volume^Porod	143	nm³

SASDFM6 – Adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5

UniProt ID: O25315 (1-598) Adenine specific DNA methyltransferase (Mod)

Sample:	Adenine specific DNA methyltransferase (Mod) tetramer, 273 kDa Helicobacter pylori protein
Buffer:	25 mM citrate, 250 mM NaCl, pH: 5.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 1

R_g^Guinier	5.0	nm
D_max	19.1	nm
Volume^Porod	316	nm³

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