SASBDB entries for UniProt ID: P61823

SASDAN2 – RNase in PBS

UniProt ID: P61823 (None-None) Ribonuclease pancreatic

Ribonuclease pancreatic experimental SAS data
GASBOR model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 15 nm3

SASDAR2 – RNase in PBS

UniProt ID: P61823 (None-None) Ribonuclease pancreatic

Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 17 nm3

SASDDL3 – Folded ribonuclease A (RNAse)

UniProt ID: P61823 (None-None) Ribonuclease pancreatic

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: phosphate buffered saline (PBS), pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 1.6 nm
Dmax 5.6 nm
VolumePorod 16 nm3

SASDDM3 – Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea

UniProt ID: P61823 (None-None) Ribonuclease pancreatic

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: 10 mM HCl, pH: 1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.3 nm
Dmax 9.0 nm

SASDEY4 – Ribonuclease A monomer from in-house SEC-SAXS

UniProt ID: P61823 (27-150) Ribonuclease pancreatic

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 10 nm3

SASDH32 – Protein Disulfide Isomerase (PDI) in Complex with Ribonuclease-A (RNase)

UniProt ID: P05307 (1-510) Protein disulfide-isomerase

UniProt ID: P61823 (27-150) Ribonuclease pancreatic

Protein disulfide-isomeraseRibonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Structural Insights on Dynamic behavior of PDI in solution environment through SAXS technique
Chandra Sekar
RgGuinier 3.0 nm
Dmax 9.1 nm
VolumePorod 85 nm3

SASDH52 – Ribonuclease A (Type III-A) in a solution environment at pH 7.5

UniProt ID: P61823 (27-150) Ribonuclease pancreatic

Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Structural Insights on Dynamic behavior of PDI in solution environment through SAXS technique
Chandra Sekar
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 20 nm3