Browse by MODEL: Ensemble

SASDDZ4 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU

Protein sex-lethal mutantRNA decaneucleotide U8GU experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
RNA decaneucleotide U8GU monomer, 3 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 3
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.2 nm
Dmax 7.8 nm
VolumePorod 34 nm3

SASDD25 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8

Protein sex-lethal mutantRNA decaneucleotide UGU8 experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8 Rg histogram
Sample: Protein sex-lethal mutant dimer, 41 kDa Drosophila melanogaster protein
RNA decaneucleotide UGU8 dimer, 6 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 12
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 41 nm3

SASDBZ6 – Draxin

Draxin experimental SAS data
Draxin Rg histogram
Sample: Draxin monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 87 nm3

SASDCY4 – RNase E 603-850

RNase E 603-850 experimental SAS data
RNase E 603-850 Rg histogram
Sample: RNase E 603-850 monomer, 30 kDa Escherichia coli protein
Buffer: 50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 5
Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes. Nucleic Acids Res 46(1):387-402 (2018)
Bruce HA, Du D, Matak-Vinkovic D, Bandyra KJ, Broadhurst RW, Martin E, Sobott F, Shkumatov AV, Luisi BF
RgGuinier 5.3 nm
Dmax 27.5 nm
VolumePorod 139 nm3

SASDCL7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390)

Cytohesin-3 experimental SAS data
Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390) Rg histogram
Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.001 mM inositol 1,3,4,5-tetrakisphosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Mar 21
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 2.8 nm
Dmax 9.3 nm
VolumePorod 64 nm3

SASDCQ7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein

Grp1 63-399 E161A Arf6 Q67L fusion protein experimental SAS data
Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein Rg histogram
Sample: Grp1 63-399 E161A Arf6 Q67L fusion protein monomer, 60 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 3.1 nm
Dmax 11.6 nm
VolumePorod 86 nm3

SASDDE6 – Photoreceptor sensory box 1 light-state (SB1-LOV-R66I)

Sensory box protein light-state (R66I) experimental SAS data
Photoreceptor sensory box 1 light-state (SB1-LOV-R66I) Rg histogram
Sample: Sensory box protein light-state (R66I) dimer, 37 kDa Pseudomonas putida protein
Buffer: 10mM Tris, 10 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 2
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein. PLoS One 13(7):e0200746 (2018)
Röllen K, Granzin J, Batra-Safferling R, Stadler AM
RgGuinier 2.6 nm
Dmax 9.2 nm
VolumePorod 58 nm3

SASDDG6 – Photoreceptor sensory box 1 dark-state (SB1-LOV-R66I)

Sensory box protein dark-state experimental SAS data
Photoreceptor sensory box 1 dark-state (SB1-LOV-R66I) Rg histogram
Sample: Sensory box protein dark-state dimer, 37 kDa Pseudomonas putida protein
Buffer: 10mM Tris, 10 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 2
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein. PLoS One 13(7):e0200746 (2018)
Röllen K, Granzin J, Batra-Safferling R, Stadler AM
RgGuinier 2.6 nm
Dmax 8.0 nm
VolumePorod 57 nm3

SASDC26 – DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210

BCR-ABL p210 fusion protein (DH-PH) experimental SAS data
DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210 Rg histogram
Sample: BCR-ABL p210 fusion protein (DH-PH) monomer, 47 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 13
Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase. Nat Commun 8(1):2101 (2017)
Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 69 nm3

SASDC23 – Colicin N WT

Colicin N experimental SAS data
Colicin N WT Rg histogram
Sample: Colicin N monomer, 43 kDa Escherichia coli protein
Buffer: 50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2012 Jun 29
The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 74 nm3