Browse by MODEL: Hybrid

SASDEN5 – AMP/fumarate-bound neanderthal adenylosuccinate lyase (ADSL)

Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 258 nm3

SASDER5 – AICAR/fumarate-bound neanderthal adenylosuccinate lyase (ADSL)

Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 252 nm3

SASDE96 – Aldehyde dehydrogenase 12 from Zea mays Extrapolated to Infinite Dilution

Aldehyde dehydrogenase 12 experimental SAS data
ALLOSMOD model
Sample: Aldehyde dehydrogenase 12 tetramer, 242 kDa Zea mays protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM TCEP, and 5% (v/v) glycerol, pH: 7.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 6
Structural and Biochemical Characterization of Aldehyde Dehydrogenase 12, the Last Enzyme of Proline Catabolism in Plants. J Mol Biol (2018)
Korasick DA, Končitíková R, Kopečná M, Hájková E, Vigouroux A, Moréra S, Becker DF, Šebela M, Tanner JJ, Kopečný D
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 351 nm3

SASDEZ6 – Gamma-crystallin S disulfide-linked dimer

Gamma-crystallin S experimental SAS data
DAMMIN model
Sample: Gamma-crystallin S dimer, 42 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at Bruker Nanostar II, Australian Nuclear Science and Technology Organisation/Australian Centre for Neutron Scattering on 2018 Feb 23
The structure and stability of the disulfide-linked γS-crystallin dimer provide insight into oxidation products associated with lens cataract formation. J Mol Biol (2018)
Thorn DC, Grosas AB, Mabbitt PD, Ray NJ, Jackson CJ, Carver JA
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 45 nm3

SASDE27 – Gamma-crystallin S monomer

Gamma-crystallin S experimental SAS data
CHIMERA model
Sample: Gamma-crystallin S monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at Bruker Nanostar II, Australian Nuclear Science and Technology Organisation/Australian Centre for Neutron Scattering on 2018 Feb 23
The structure and stability of the disulfide-linked γS-crystallin dimer provide insight into oxidation products associated with lens cataract formation. J Mol Biol (2018)
Thorn DC, Grosas AB, Mabbitt PD, Ray NJ, Jackson CJ, Carver JA
RgGuinier 1.8 nm
Dmax 5.9 nm
VolumePorod 27 nm3

SASDEU8 – GTPase Elongation Factor like-1 protein (yeast EFL1)

Ribosome assembly protein 1 experimental SAS data
DAMFILT model
Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
RgGuinier 4.7 nm
Dmax 15.8 nm
VolumePorod 258 nm3

SASDEV8 – Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1)

Ribosome maturation protein SDO1 experimental SAS data
MULTIFOXS model
Sample: Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
RgGuinier 2.7 nm
Dmax 8.5 nm

SASDEW8 – Shwachman-Bodian-Diamond Syndrome protein (SDO1) with domains 2 and 3

Ribosome maturation protein SDO1 experimental SAS data
DAMFILT model
Sample: Ribosome maturation protein SDO1 monomer, 17 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 14
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
RgGuinier 2.1 nm
Dmax 6.2 nm
VolumePorod 25 nm3

SASDE47 – Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 2 mg/ml

Aldehyde dehydrogenase 16 from Loktanella sp. experimental SAS data
PYMOL model
Sample: Aldehyde dehydrogenase 16 from Loktanella sp. dimer, 161 kDa Loktanella sp. 3ANDIMAR09 protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 2.0% glycerol, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Dec 13
Crystal Structure of Aldehyde Dehydrogenase 16 Reveals Trans-Hierarchical Structural Similarity and a New Dimer. J Mol Biol (2018)
Liu LK, Tanner JJ
RgGuinier 3.6 nm
Dmax 10.9 nm
VolumePorod 202 nm3

SASDE57 – Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 4 mg/ml

Aldehyde dehydrogenase 16 from Loktanella sp. experimental SAS data
PYMOL model
Sample: Aldehyde dehydrogenase 16 from Loktanella sp. dimer, 161 kDa Loktanella sp. 3ANDIMAR09 protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 2.0% glycerol, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Dec 13
Crystal Structure of Aldehyde Dehydrogenase 16 Reveals Trans-Hierarchical Structural Similarity and a New Dimer. J Mol Biol (2018)
Liu LK, Tanner JJ
RgGuinier 3.6 nm
Dmax 11.2 nm
VolumePorod 204 nm3