Browse by MODEL: Hybrid

SASDAH2 – Myoglobin in PBS

Myoglobin experimental SAS data
CRYSOL model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 20
Standard proteins
Darja Ruskule
RgGuinier 1.7 nm
Dmax 5.0 nm
VolumePorod 32 nm3

SASDAK2 – Myoglobin in PBS

Myoglobin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 20
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 32 nm3

SASDAL2 – Ovalbumin in PBS

Ovalbumin experimental SAS data
DAMMIN model
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 74 nm3

SASDAN2 – Bovine pancreatic ribonuclease A in PBS (WAXS)

Ribonuclease pancreatic experimental SAS data
GASBOR model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 15 nm3

SASDAQ2 – Ubiquitin in sodium acetate

Ubiquitin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ubiquitin monomer, 9 kDa Bos taurus protein
Buffer: 40 mM Sodium acetate 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.3 nm
Dmax 4.9 nm
VolumePorod 12 nm3

SASDAR2 – Bovine pancreatic ribonuclease A in PBS

Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 17 nm3

SASDAK6 – Glucose Isomerase

Xylose isomerase experimental SAS data
DAMMIF model
Sample: Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer: PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 24
Standard proteins
Erica Valentini
RgGuinier 3.4 nm
Dmax 9.7 nm
VolumePorod 293 nm3

SASDA58 – UL26N of pseudorabies virus

VP24 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: VP24 dimer, 53 kDa Suid herpesvirus 1 protein
Buffer: 50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease. PLoS Pathog 11(7):e1005045 (2015)
Zühlsdorf M, Werten S, Klupp BG, Palm GJ, Mettenleiter TC, Hinrichs W
RgGuinier 2.6 nm
Dmax 10.6 nm
VolumePorod 73 nm3

SASDA37 – Surface Protein G (SasG) EG5 repeat protein G51-G52

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 24 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 4.7 nm
Dmax 19.0 nm
VolumePorod 29 nm3

SASDA47 – Surface Protein G (SasG) EG5 repeat protein G51-G53

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 39 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 7.7 nm
Dmax 30.5 nm
VolumePorod 49 nm3