Browse by MODEL: Hybrid

SASDA57 – Surface Protein G (SasG) EG5 repeat protein G51-G54

Surface protein G experimental SAS data
GASBOR model
Sample: Surface protein G monomer, 53 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 9.7 nm
Dmax 38.5 nm
VolumePorod 58 nm3

SASDA67 – Surface Protein G (SasG) EG5 repeat protein G51-G55

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 67 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 12.0 nm
Dmax 48.0 nm
VolumePorod 87 nm3

SASDA77 – Surface Protein G (SasG) EG5 repeat protein G51-G56

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 81 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 14.1 nm
Dmax 57.0 nm
VolumePorod 89 nm3

SASDA87 – Surface Protein G (SasG) EG5 repeat protein G51-G57

Surface protein G experimental SAS data
SASREF model
Sample: Surface protein G monomer, 95 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris 200 mM NaCl 1 mM EDTA 20 mM Tris.Cl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 12
Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein. Nat Commun 6:7271 (2015)
Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J
RgGuinier 15.9 nm
Dmax 63.0 nm
VolumePorod 122 nm3

SASDA96 – Lysozyme

Lysozyme C experimental SAS data
DAMMIF model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 20 mM Sodium Acetate/HEPES, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Feb 17
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 24 nm3

SASDAA6 – Human serum albumin monomer and mixtures

Human serum albumin monomer experimental SAS data
DAMMIF model
Sample: Human serum albumin monomer monomer, 66 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 103 nm3

SASDAB6 – Xylose Isomerase

Xylose Isomerase experimental SAS data
DAMMIF model
Sample: Xylose Isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 20 mM HEPES 200 mM Na2SO4 50 mM K2SO4 500 % v/v D2O 1 mM MgCl2, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 10
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 3.2 nm
Dmax 9.1 nm
VolumePorod 234 nm3

SASDAT6 – Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains

Integrin beta-4 experimental SAS data
DAMMIF model
Sample: Integrin beta-4 monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 6
Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4. Acta Crystallogr D Biol Crystallogr 71(Pt 4):969-85 (2015)
Alonso-García N, García-Rubio I, Manso JA, Buey RM, Urien H, Sonnenberg A, Jeschke G, de Pereda JM
RgGuinier 2.2 nm
Dmax 7.0 nm
VolumePorod 32 nm3

SASDAA7 – Heterotetramer of histidine protein kinase and response regulator

Histidine protein kinaseResponse regulator experimental SAS data
BUNCH model
Sample: Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 4.0 nm
Dmax 16.0 nm
VolumePorod 175 nm3

SASDAB7 – Complex ComE-comcde

comcdeResponse regulator experimental SAS data
OTHER model
Sample: Comcde, 24 kDa Streptococcus pneumoniae DNA
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 11
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 122 nm3