Browse by MODEL: Hybrid

SASDQW5 – C-terminal-binding protein 1 (CtBP1)

C-terminal-binding protein 1 experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.3 nm
Dmax 14.0 nm
VolumePorod 279 nm3

SASDQX5 – C-terminal binding protein 1 mutant (CtBP1: C134Y, N138R, R141E, L150W)

C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W), 48 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 154 nm3

SASDQY5 – C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)

C-terminal-binding protein 1 (R266A, D290A, E295A, H315A) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 (R266A, D290A, E295A, H315A), 47 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 161 nm3

SASDQC6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465)

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465) monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 7.4 nm
Dmax 28.0 nm
VolumePorod 978 nm3

SASDUW6 – Alarmin release inhibitor (HpARI2), full-length

Alarmin release inhibitor (Δ1-62) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-62) monomer, 26 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.6 nm
Dmax 9.9 nm
VolumePorod 31 nm3

SASDUX6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3

Alarmin release inhibitor (Δ1-125; N175Q, N190Q) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-125; N175Q, N190Q) monomer, 19 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 28 nm3

SASDUY6 – Alarmin release inhibitor (HpARI2), full length, bound to Interleukin-33

Alarmin release inhibitor (Δ1-62)Interleukin-33 (L179V) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-62) monomer, 26 kDa Heligmosomoides polygyrus protein
Interleukin-33 (L179V) monomer, 18 kDa Mus musculus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.7 nm
Dmax 9.7 nm
VolumePorod 62 nm3

SASDUZ6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3 bound to Interleukin-33

Interleukin-33 (L179V)Alarmin release inhibitor (Δ1-125; N175Q, N190Q) experimental SAS data
REFMAC model
Sample: Interleukin-33 (L179V) monomer, 18 kDa Mus musculus protein
Alarmin release inhibitor (Δ1-125; N175Q, N190Q) monomer, 19 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.5 nm
Dmax 8.3 nm
VolumePorod 58 nm3

SASDSE7 – Human CDAN1-interacting nuclease 1 (CDIN1): analysis of SEC peak

CDAN1-interacting nuclease 1 experimental SAS data
ALPHAFOLD model
Sample: CDAN1-interacting nuclease 1 dimer, 67 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Sep 2
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 3.0 nm
Dmax 8.2 nm
VolumePorod 84 nm3

SASDSF7 – Human Codanin1 (C-terminus): analysis of second monomeric SEC peak

Codanin-1 experimental SAS data
ALPHAFOLD model
Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 2.7 nm
Dmax 7.5 nm
VolumePorod 54 nm3