Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDGK6 – Ubiquitin activating enzyme 5 (4.4 mg/ml)

Ubiquitin-like modifier-activating enzyme 5 experimental SAS data

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M

R_g^Guinier	3.1	nm
D_max	12.0	nm
Volume^Porod	104	nm³

SASDGL6 – Ubiquitin activating enzyme 5 (3.6 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	97	nm³

SASDGM6 – Ubiquitin activating enzyme 5 (1.9 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	2.9	nm
D_max	9.8	nm
Volume^Porod	94	nm³

SASDL73 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 2 mg/mL + UFM1 0.5 mg/mL)

Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	2.9	nm
D_max	11.0	nm

SASDL83 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 3 mg/mL + UFM1 0.75 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.0	nm
D_max	12.0	nm

SASDL93 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 4 mg/mL + UFM1 1.0 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm

SASDLA3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 5 mg/mL + UFM1 1.25 mg/ml)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	100	nm³

SASDLB3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 6 mg/mL + UFM1 1.5 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	103	nm³

SASDLC3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 7 mg/mL + UFM1 1.75 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	104	nm³

SASDLD3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 8 mg/mL + UFM1 2 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	106	nm³

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