Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDLE3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 9 mg/mL + UFM1 2.25 mg/mL)

Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M

R_g^Guinier	3.2	nm
D_max	14.5	nm
Volume^Porod	111	nm³

SASDM82 – Ubiquitin-fold modifier 1

Ubiquitin fold modifer 1 experimental SAS data

Sample:	Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	16	nm³

SASDK74 – Streptococcus agalactiae transcription factor BusR - RCK_C domain

Transcriptional repressor BusR RCK_C domain experimental SAS data

Sample:	Transcriptional repressor BusR RCK_C domain dimer, 22 kDa Streptococcus agalactiae serotype … protein
Buffer:	100 mM NaCl, 30mM Hepes, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2

BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G

R_g^Guinier	1.9	nm
D_max	6.4	nm
Volume^Porod	44	nm³

SASDK94 – Streptococcus agalactiae transcription factor BusR dsDNA complex

Transcriptional repressor BusRBusR Recognition sequence experimental SAS data

Sample:	Transcriptional repressor BusR tetramer, 95 kDa Streptococcus agalactiae protein BusR Recognition sequence monomer, 28 kDa synthetic construct DNA
Buffer:	20mM HEPES, pH6.5, 100mM NaCl, 3% glycerol (v/v), pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2

BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture. Nucleic Acids Res (2021)
Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G

R_g^Guinier	4.3	nm
D_max	14.2	nm
Volume^Porod	210	nm³

SASDKF6 – C2AB construct of synaptotagmin-1 (SYT1) with E395-397A mutations at the linker

Sample:	Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein
Buffer:	50 mM Tris, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jun 11

The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites Life Science Alliance 4(10):e202101152 (2021)
Benavente J, Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A

R_g^Guinier	3.1	nm
D_max	12.1	nm
Volume^Porod	59	nm³

SASDKG6 – SMP2C2A construct of synaptotagmin-1 (SYT1)

Synaptotagmin-1 (SYT1-SMP2C2A) experimental SAS data

Sample:	Synaptotagmin-1 (SYT1-SMP2C2A) monomer, 72 kDa Escherichia coli protein
Buffer:	20 mM Tris, 100 mM NaCl, 5% glycerol, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jun 11

R_g^Guinier	4.2	nm
D_max	17.8	nm
Volume^Porod	138	nm³

SASDKJ9 – C2AB construct of synaptotagmin-1 (SYT1)

Sample:	Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein
Buffer:	50 mM Tris, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Feb 16

R_g^Guinier	2.8	nm
D_max	12.6	nm
Volume^Porod	50	nm³

SASDKK9 – C2AB construct of synaptotagmin-1 (SYT1) in presence of Ca cations

Sample:	Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein
Buffer:	50 mM Tris, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Feb 16

R_g^Guinier	2.9	nm
D_max	13.8	nm
Volume^Porod	54	nm³

SASDJD2 – Human respiratory syncytial virus A (RSV) phosphoprotein oligomerization domain

Sample:	Phosphoprotein tetramer, 18 kDa Respiratory syncytial virus protein
Buffer:	20 mM Na phosphate 100 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jun 21

A Structural and Dynamic Analysis of the Partially Disordered Polymerase-Binding Domain in RSV Phosphoprotein Biomolecules 11(8):1225 (2021)
Cardone C, Caseau C, Bardiaux B, Thureaux A, Galloux M, Bajorek M, Eléouët J, Litaudon M, Bontems F, Sizun C

R_g^Guinier	2.1	nm
D_max	7.6	nm
Volume^Porod	33	nm³

SASDLQ2 – PfMDH L-lactate dehydrogenase, apo

L-lactate dehydrogenase experimental SAS data

Sample:	L-lactate dehydrogenase tetramer, 141 kDa Plasmodium falciparum protein
Buffer:	100 mM Na-phosphate buffer, 400 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Xenocs Xeuss 2.0 with MetalJet, Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University on 2019 Jul 3

A fragment-based approach identifies an allosteric pocket that impacts malate dehydrogenase activity Communications Biology 4(1) (2021)
Reyes Romero A, Lunev S, Popowicz G, Calderone V, Gentili M, Sattler M, Plewka J, Taube M, Kozak M, Holak T, Dömling A, Groves M

R_g^Guinier	3.4	nm
D_max	10.5	nm
Volume^Porod	211	nm³

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