Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDJG4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD)

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Sample:	Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	25 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 1

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 Nature Communications 11(1) (2020)
Custódio T, Das H, Sheward D, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C

R_g^Guinier	3.0	nm
D_max	13.1	nm
Volume^Porod	64	nm³

SASDJH4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) bound to the synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Sample:	Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	25 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 10

R_g^Guinier	3.5	nm
D_max	15.1	nm
Volume^Porod	87	nm³

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data

Sample:	Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C

R_g^Guinier	5.4	nm
D_max	12.5	nm
Volume^Porod	679	nm³

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data

Sample:	Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	1.8	nm
D_max	5.1	nm
Volume^Porod	37	nm³

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	98	nm³

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	4.0	nm
D_max	13.2	nm
Volume^Porod	211	nm³

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data

Sample:	Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	3.3	nm
D_max	9.3	nm
Volume^Porod	201	nm³

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23

R_g^Guinier	2.8	nm
D_max	8.3	nm
Volume^Porod	95	nm³

SASDJ92 – S. epidermidis extracellular matrix binding protein (Embp) F-repeats

Extracellular matrix binding protein F-repeats experimental SAS data

Sample:	Extracellular matrix binding protein F-repeats, 70 kDa Staphylococcus epidermidis protein
Buffer:	50 mM MES, 150 mM NaCl, pH: 6
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 15

A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism. mBio 11(5) (2020)
Büttner H, Perbandt M, Kohler T, Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H

R_g^Guinier	7.0	nm
D_max	28.0	nm

SASDJA2 – S. epidermidis extracellular matrix binding protein (Embp) FG-repeats

Extracellular matrix binding protein FG-repeats experimental SAS data

Sample:	Extracellular matrix binding protein FG-repeats, 85 kDa Staphylococcus epidermidis protein
Buffer:	PBS, 136.5 mM NaCl, 2.65 mM KCl, 8.3 mM Na2HPO4, 2.65 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14

R_g^Guinier	10.7	nm
D_max	40.0	nm

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