Browse by MODEL: No model

SASDE86 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A

ATP-citrate lyase experimental SAS data
ATP-citrate lyase Kratky plot
Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.7 nm
Dmax 17.5 nm
VolumePorod 791 nm3

SASDFA3 – Human ATP-citrate synthase (ACLY) full length in HBS

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 19.0 nm
VolumePorod 765 nm3

SASDFB3 – Human ATP-citrate synthers (ACLY) full length in HBS + Citrate

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.2 nm
Dmax 19.0 nm
VolumePorod 787 nm3

SASDDQ5 – Human metaphase chromosomes in 17mM magnesium chloride

Metaphase chromosomes in 17 mM magnesium chloride experimental SAS data
Metaphase chromosomes in 17 mM magnesium chloride Kratky plot
Sample: Metaphase chromosomes in 17 mM magnesium chloride monomer, 0 kDa Homo sapiens
Buffer: 10 mM PIPES, 17 mM magnesium chloride, 20 mM sodium chloride, 120 mM potassium chloride, 40% glycerol, pH: 7.2
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 May 23
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure. EMBO J 38(7) (2019)
Chicano A, Crosas E, Otón J, Melero R, Engel BD, Daban JR

SASDDR5 – Human metaphase chromosomes in 5 mM magnesium chloride

Human metaphase chromosomes in 5 mM magnesium chloride experimental SAS data
Human metaphase chromosomes in 5 mM magnesium chloride Kratky plot
Sample: Human metaphase chromosomes in 5 mM magnesium chloride monomer, 0 kDa Homo sapiens
Buffer: 10 mM PIPES, 5 mM magnesium chloride, 10 mM sodium chloride, 120 mM potassium chloride, 40% glycerol, pH: 7.2
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 Jun 6
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure. EMBO J 38(7) (2019)
Chicano A, Crosas E, Otón J, Melero R, Engel BD, Daban JR

SASDDS5 – Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride

Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride experimental SAS data
Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride Kratky plot
Sample: Human metaphase chromosomes in 1 mM hexaamminecobalt(III) chloride monomer, 0 kDa Homo sapiens
Buffer: 10 mM PIPES, hexaamminecobalt(III) chloride, 40% glycerol, pH: 7.2
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2013 Sep 12
Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure. EMBO J 38(7) (2019)
Chicano A, Crosas E, Otón J, Melero R, Engel BD, Daban JR

SASDE48 – Toxin GraT

Putative killer protein, Toxin GraT. experimental SAS data
Putative killer protein, Toxin GraT. Kratky plot
Sample: Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein
Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Sep 21
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
RgGuinier 1.5 nm
Dmax 5.5 nm
VolumePorod 18 nm3

SASDE58 – Complex of toxin GraT with antotoxin GraA

Putative killer protein, Toxin GraT.antitoxin GraA, antidote of toxin GraTPutative killer protein, Toxin GraT experimental SAS data
Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT Kratky plot
Sample: Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein
Antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein
Putative killer protein, Toxin GraT monomer, 11 kDa Pseudomonas putida protein
Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 29
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
RgGuinier 2.3 nm
Dmax 7.9 nm
VolumePorod 63 nm3

SASDE68 – Two dimers of antitoxin GraA in complex with the grta operator

GraTA operator regionantitoxin GraA, antidote of toxin GraTatitoxin GraA, antidote of GraT. experimental SAS data
GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Kratky plot
Sample: GraTA operator region monomer, 20 kDa DNA
Antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein
Atitoxin GraA, antidote of GraT. dimer, 23 kDa Pseudomonas putida protein
Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Dec 17
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
RgGuinier 2.9 nm
Dmax 10.3 nm
VolumePorod 94 nm3

SASDFV8 – Apolipoprotein E2 (ApoE2) bound to 4 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data
Apolipoprotein E2 Heparin Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 6.2 nm
Dmax 20.7 nm
VolumePorod 504 nm3