|
|
|
|
|
| Sample: |
Cold shock-like protein monomer, 7 kDa Thermotoga maritima protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
|
| Buffer: |
PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Jun 15
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
| RgGuinier |
2.2 |
nm |
| Dmax |
8.3 |
nm |
| VolumePorod |
9 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
|
| Buffer: |
PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Jun 15
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
| RgGuinier |
3.6 |
nm |
| Dmax |
13.0 |
nm |
| VolumePorod |
34 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
|
| Buffer: |
PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Jun 15
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
| RgGuinier |
3.2 |
nm |
| Dmax |
13.9 |
nm |
| VolumePorod |
31 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Nuclear pore complex protein Nup98-Nup96 monomer, 15 kDa Homo sapiens protein
|
| Buffer: |
PBS, 10 mM DTT, pH: 7.4 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2015 Jun 24
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
| RgGuinier |
2.9 |
nm |
| Dmax |
10.4 |
nm |
| VolumePorod |
27 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Nucleoporin NSP1 monomer, 18 kDa Saccharomyces cerevisiae protein
|
| Buffer: |
PBS, 10 mM DTT, pH: 7.4 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2015 Jun 24
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
| RgGuinier |
4.1 |
nm |
| Dmax |
15.0 |
nm |
| VolumePorod |
45 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Major viral transcription factor ICP4 dimer, 49 kDa Human alphaherpesvirus 1 protein
|
| Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.4 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2015 Jul 27
|
The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Nucleic Acids Res 45(13):8064-8078 (2017)
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
|
| RgGuinier |
2.9 |
nm |
| Dmax |
12.7 |
nm |
|
|
|
|
|
|
|
| Sample: |
Immediate Early 3 dimer, 12 kDa DNA
|
| Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.4 |
| Experiment: |
SAXS
data collected at BM29, ESRF on 2015 Nov 12
|
The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Nucleic Acids Res 45(13):8064-8078 (2017)
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
|
| RgGuinier |
1.9 |
nm |
| Dmax |
6.9 |
nm |
|
|
|
|
|
|
|
| Sample: |
Bcl-2-like protein FPV039 monomer, 17 kDa Fowlpox virus protein
Uncharacterized protein (BAK1) monomer, 3 kDa Gallus gallus protein
|
| Buffer: |
20 mM trisodium citrate pH, 200 mM NaCl, pH: 6 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Oct 2
|
Structural basis of apoptosis inhibition by the fowlpox virus protein FPV039.
J Biol Chem 292(22):9010-9021 (2017)
Anasir MI, Caria S, Skinner MA, Kvansakul M
|
|
|
|
|
|
|
|
| Sample: |
Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
|
| Buffer: |
20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Jul 2
|
TIA-1 RRM23 binding and recognition of target oligonucleotides.
Nucleic Acids Res 45(8):4944-4957 (2017)
Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA
|
| RgGuinier |
2.3 |
nm |
| Dmax |
8.8 |
nm |
| VolumePorod |
26 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
RNA (ACUCCUUUUU) monomer, 1 kDa RNA
|
| Buffer: |
20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27
|
TIA-1 RRM23 binding and recognition of target oligonucleotides.
Nucleic Acids Res 45(8):4944-4957 (2017)
Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA
|
| RgGuinier |
2.2 |
nm |
| Dmax |
6.6 |
nm |
| VolumePorod |
30 |
nm3 |
|
|