Browse by MACROMOLECULE type: protein

SASDCY9 – Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii

Calvin cycle protein CP12, chloroplastic experimental SAS data
Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii Rg histogram
Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein
Buffer: 50 mM phosphate buffer, 50 mM NaCl, 20 mM oxidized DTT, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 3
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 22 nm3

SASDCZ9 – Glyceraldehyde-3-phosphate dehydrogenase from C. reinhardtii

Glyceraldehyde-3-phosphate dehydrogenase experimental SAS data
MODELLER model
Sample: Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein
Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 7
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 206 nm3

SASDD22 – Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)

Calvin cycle protein CP12, chloroplasticGlyceraldehyde-3-phosphate dehydrogenase experimental SAS data
Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii) Rg histogram
Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein
Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein
Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2010 Dec 8
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 3.8 nm
Dmax 19.0 nm
VolumePorod 251 nm3

SASDDX4 – Wild-type Protein sex-lethal

Protein sex-lethal experimental SAS data
Protein sex-lethal Kratky plot
Sample: Protein sex-lethal monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 26 nm3

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

Protein sex-lethal mutant experimental SAS data
Protein sex-lethal mutant with 10GS-linker Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 2
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 27 nm3

SASDB78 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I)

Probable ATP-dependent RNA helicase DDX58 experimental SAS data
BUNCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2012 Apr 6
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.3 nm
Dmax 14.0 nm
VolumePorod 186 nm3

SASDB88 – Probable ATP-dependent RNA helicase DDX58 (Full-length RIG-I) plus ADP-AlFx

Probable ATP-dependent RNA helicase DDX58 experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 monomer, 108 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 4.2 nm
Dmax 15.6 nm
VolumePorod 190 nm3

SASDBG8 – Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I)

Probable ATP-dependent RNA helicase DDX58 (without CARDs) experimental SAS data
BUNCH model
Sample: Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 114 nm3

SASDBH8 – Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I) plus ADP-AlFx

Probable ATP-dependent RNA helicase DDX58 (without CARDs) experimental SAS data
EOM/RANCH model
Sample: Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 132 nm3

SASDBZ6 – Draxin

Draxin experimental SAS data
Draxin Rg histogram
Sample: Draxin monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
Liu Y, Bhowmick T, Liu Y, Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 87 nm3