Browse by ORGANISM: Homo sapiens (Human)

SASDEN4 – Protein tyrosine phosphatase SHP2

Tyrosine-protein phosphatase non-receptor type 11 experimental SAS data
ALLOSMOD model
Sample: Tyrosine-protein phosphatase non-receptor type 11 monomer, 61 kDa Homo sapiens protein
Buffer: 50 mM ADA, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2016 Mar 28
Mechanism of activating mutations and allosteric drug inhibition of the phosphatase SHP2. Nat Commun 9(1):4507 (2018)
Pádua RAP, Sun Y, Marko I, Pitsawong W, Stiller JB, Otten R, Kern D
RgGuinier 2.7 nm
Dmax 8.9 nm

SASDEP4 – Protein tyrosine phosphatase SHP2 with E76K activating mutation

Tyrosine-protein phosphatase non-receptor type 11 E76K experimental SAS data
ALLOSMOD model
Sample: Tyrosine-protein phosphatase non-receptor type 11 E76K monomer, 61 kDa Homo sapiens protein
Buffer: 50 mM ADA, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2016 Mar 28
Mechanism of activating mutations and allosteric drug inhibition of the phosphatase SHP2. Nat Commun 9(1):4507 (2018)
Pádua RAP, Sun Y, Marko I, Pitsawong W, Stiller JB, Otten R, Kern D
RgGuinier 2.9 nm
Dmax 9.5 nm

SASDE25 – Human insulin hexamer from in-house SEC-SAXS

Human insulin experimental SAS data
DAMFILT model
Sample: Human insulin hexamer, 35 kDa Homo sapiens protein
Buffer: 7 mM sodium phosphate, 60 mM phenol, 200 µM Zn(CH3COO)2, 23 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 26
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.0 nm
Dmax 5.9 nm
VolumePorod 39 nm3

SASDDN6 – Human stress-inducible heat shock 70 kDa protein 1, Hsp70 (Hspa1a) T204A mutant dimer, in the presence of ATP

Heat shock 70 kDa protein 1 experimental SAS data
MODELLER model
Sample: Heat shock 70 kDa protein 1 dimer, 147 kDa Homo sapiens protein
Buffer: 50mM HEPES, 150mM KCH3COO, 2mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2018 Feb 20
Human stress-inducible Hsp70 has a high propensity to form ATP-dependent antiparallel dimers that are differentially regulated by co-chaperone binding. Mol Cell Proteomics (2018)
Trcka F, Durech M, Vankova P, Chmelik J, Martinkova V, Hausner J, Kadek A, Marcoux J, Klumpler T, Vojtesek B, Muller P, Man P
RgGuinier 4.0 nm
Dmax 11.6 nm
VolumePorod 248 nm3

SASDD85 – PDZK1 Domain 1-4

Uncharacterized protein C1orf159 experimental SAS data
GASBOR model
Sample: Uncharacterized protein C1orf159 monomer, 53 kDa Homo sapiens protein
Buffer: 20mM Hepes 150 NaCl 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 28
Probing the Architecture of a Multi-PDZ Domain Protein: Structure of PDZK1 in Solution. Structure 26(11):1522-1533.e5 (2018)
Hajizadeh NR, Pieprzyk J, Skopintsev P, Flayhan A, Svergun DI, Löw C
RgGuinier 3.9 nm
Dmax 15.0 nm
VolumePorod 120 nm3

SASDEH6 – BS69 (CC-MYND: Zinc finger MYND domain-containing protein 11, amino acids 480-602)

Zinc finger MYND domain-containing protein 11 experimental SAS data
DAMMIN model
Sample: Zinc finger MYND domain-containing protein 11 tetramer, 60 kDa Homo sapiens protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth (2018)
Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
RgGuinier 5.3 nm
Dmax 16.9 nm
VolumePorod 83 nm3

SASDEJ6 – Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino acids 381-455) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)

Epstein-Barr nuclear antigen 2Zinc finger MYND domain-containing protein 11 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Zinc finger MYND domain-containing protein 11 tetramer, 60 kDa Homo sapiens protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth (2018)
Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
RgGuinier 4.6 nm
Dmax 13.8 nm
VolumePorod 72 nm3

SASDEK6 – Complex of Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422) and BS69 (Zinc finger MYND domain-containing protein 11: CC-MYND, amino acids 480-602)

Epstein-Barr nuclear antigen 2Zinc finger MYND domain-containing protein 11 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Zinc finger MYND domain-containing protein 11 hexamer, 90 kDa Homo sapiens protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth (2018)
Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
RgGuinier 4.7 nm
Dmax 14.5 nm
VolumePorod 239 nm3

SASDEP7 – Filamin A Ig-like domains 3-5 P637Q mutant (FLNa3-5 P637Q)

Filamin A Ig.like domains 3-5 P637Q mutant experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Filamin A Ig.like domains 3-5 P637Q mutant monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Non-syndromic Mitral Valve Dysplasia Mutation Changes the Force Resilience and Interaction of Human Filamin A. Structure (2018)
Haataja TJK, Bernardi RC, Lecointe S, Capoulade R, Merot J, Pentikäinen U
RgGuinier 2.2 nm
Dmax 7.4 nm
VolumePorod 39 nm3

SASDEQ7 – Filamin A Ig-like domains 3-5 (FLNa3-5)

Filamin A Ig-like domains 3-5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Filamin A Ig-like domains 3-5 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Non-syndromic Mitral Valve Dysplasia Mutation Changes the Force Resilience and Interaction of Human Filamin A. Structure (2018)
Haataja TJK, Bernardi RC, Lecointe S, Capoulade R, Merot J, Pentikäinen U
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 39 nm3