Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDBT3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CE3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data

Sample:	Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8

RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
Hodge CD, Ismail IH, Edwards RA, Hura GL, Xiao AT, Tainer JA, Hendzel MJ, Glover JN

R_g^Guinier	4.3	nm
D_max	18.9	nm
Volume^Porod	111	nm³

SASDBU3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CUbiquitin-conjugating enzyme E2 variant 2E3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data

Sample:	Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 variant 2 dimer, 34 kDa Homo sapiens protein E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein
Buffer:	20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8

R_g^Guinier	5.2	nm
D_max	23.8	nm
Volume^Porod	192	nm³

SASDBN2 – Human linear diubiquitin

Linear di-ubiquitin experimental SAS data

Sample:	Linear di-ubiquitin monomer, 17 kDa Homo sapiens protein
Buffer:	50 mM Tris 150 mM NaCl 1 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at 5C, Pohang Accelerator Laboratory on 2014 Nov 3

New conformations of linear polyubiquitin chains from crystallographic and solution-scattering studies expand the conformational space of polyubiquitin. Acta Crystallogr D Struct Biol 72(Pt 4):524-35 (2016)
Thach TT, Shin D, Han S, Lee S

R_g^Guinier	2.1	nm
D_max	6.6	nm
Volume^Porod	20	nm³

SASDBP2 – Human linear triubiquitin

Human linear tri-ubiquitin experimental SAS data

Sample:	Human linear tri-ubiquitin monomer, 26 kDa Homo sapiens protein
Buffer:	50 mM Tris 150mM NaCl 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 5C, Pohang Accelerator Laboratory on 2014 Nov 3

R_g^Guinier	2.5	nm
D_max	8.6	nm
Volume^Porod	36	nm³

SASDBQ2 – Human linear tetraubiquitin

Human linear tetra-ubiquitin experimental SAS data

Human linear tetraubiquitin Rg histogram

Sample:	Human linear tetra-ubiquitin monomer, 34 kDa Homo sapiens protein
Buffer:	50 mM Tris 150mM NaCl 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 5C, Pohang Accelerator Laboratory on 2014 Nov 3

R_g^Guinier	3.1	nm
D_max	11.2	nm
Volume^Porod	49	nm³

SASDMU4 – Retinoic acid receptor RXR-alpha ligand binding domain (LBD)

Retinoic acid receptor RXR-alpha experimental SAS data

Sample:	Retinoic acid receptor RXR-alpha dimer, 51 kDa Homo sapiens protein
Buffer:	20 mM Tris, 100 mM NaCl, 100 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 7

Solution Behavior of the Intrinsically Disordered N-Terminal Domain of Retinoid X Receptor α in the Context of the Full-Length Protein Biochemistry 55(12):1741-1748 (2016)
Belorusova A, Osz J, Petoukhov M, Peluso-Iltis C, Kieffer B, Svergun D, Rochel N

R_g^Guinier	2.5	nm
D_max	8.3	nm
Volume^Porod	60	nm³

SASDMV4 – Full-length Retinoic acid receptor RXR-alpha in complex with Ramp2 DNA

Retinoic acid receptor RXR-alphaRamp2 DNA experimental SAS data

Sample:	Retinoic acid receptor RXR-alpha dimer, 102 kDa Homo sapiens protein Ramp2 DNA monomer, 11 kDa DNA
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 May 7

R_g^Guinier	4.4	nm
D_max	13.9	nm
Volume^Porod	161	nm³

SASDMW4 – Retinoic acid receptor RXR-alpha N-terminal and DNA binding domains (DBD) in complex with Ramp2 DNA

Ramp2 DNARetinoic acid receptor RXR-alpha experimental SAS data

Sample:	Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 44 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Jul 17

R_g^Guinier	4.3	nm
D_max	14.3	nm
Volume^Porod	89	nm³

SASDMX4 – Retinoic acid receptor RXR-alpha DNA binding (DBD) and ligand binding (LBD) domains in complex with Ramp2 DNA

Sample:	Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 74 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 7

R_g^Guinier	3.6	nm
D_max	12.2	nm

SASDMY4 – Retinoic acid receptor RXR-alpha DNA binding domain (DBD) in complex with Ramp2

Sample:	Ramp2 DNA monomer, 11 kDa DNA Retinoic acid receptor RXR-alpha dimer, 20 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 50 mM KCl, 5% glycerol, 2 mM Chaps, and 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Oct 7

R_g^Guinier	1.9	nm
D_max	5.8	nm

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