Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDLD3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 8 mg/mL + UFM1 2 mg/mL)

Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M

R_g^Guinier	3.1	nm
D_max	13.0	nm
Volume^Porod	106	nm³

SASDLE3 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 9 mg/mL + UFM1 2.25 mg/mL)

Sample:	Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29

R_g^Guinier	3.2	nm
D_max	14.5	nm
Volume^Porod	111	nm³

SASDLE5 – RORg2 bound to a Classic-RORgamma Response Element

Retinoid-related orphan receptor-gammaClassic-RORgamma Response Element experimental SAS data

RORg2 bound to a Classic-RORgamma Response Element Rg histogram

Sample:	Retinoid-related orphan receptor-gamma monomer, 56 kDa Homo sapiens protein Classic-RORgamma Response Element dimer, 19 kDa Homo sapiens DNA
Buffer:	25 mM HEPES, 150 mM TCEP, 2% Glycerol, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 20

Conformational Changes of RORγ During Response Element Recognition and Coregulator Engagement Journal of Molecular Biology :167258 (2021)
Strutzenberg T, Zhu Y, Novick S, Garcia-Ordonez R, Doebelin C, He Y, Ra Chang M, Kamenecka T, Edwards D, Griffin P

R_g^Guinier	5.5	nm
D_max	22.9	nm
Volume^Porod	132	nm³

SASDLF5 – RORg2 bound to a Variant-RORgamma Response Element

Retinoid-related orphan receptor-gammaVariant-RORgamma Response Element experimental SAS data

RORg2 bound to a Variant-RORgamma Response Element Rg histogram

Sample:	Retinoid-related orphan receptor-gamma monomer, 56 kDa Homo sapiens protein Variant-RORgamma Response Element dimer, 18 kDa Homo sapiens DNA
Buffer:	25 mM HEPES, 150 mM TCEP, 2% Glycerol, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 20

R_g^Guinier	4.4	nm
D_max	22.1	nm
Volume^Porod	112	nm³

SASDM82 – Ubiquitin-fold modifier 1

Ubiquitin fold modifer 1 experimental SAS data

Sample:	Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	16	nm³

SASDJG8 – Monomeric neurogenic locus notch homolog protein 1 (Notch1) full ectodomain

Neurogenic locus notch homolog protein 1 full ectodomain experimental SAS data

Neurogenic locus notch homolog protein 1 full ectodomain Kratky plot

Sample:	Neurogenic locus notch homolog protein 1 full ectodomain monomer, 183 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC

R_g^Guinier	10.5	nm
D_max	38.0	nm
Volume^Porod	1000	nm³

SASDL82 – Human acetylcholinesterase, apo

acetylcholinesteraseacetylcholinesterase experimental SAS data

Sample:	Acetylcholinesterase dimer, 120 kDa Homo sapiens protein Acetylcholinesterase monomer, 60 kDa Homo sapiens protein
Buffer:	50 mM Tris/HCl, 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2015 May 29

Covalent inhibition of hAChE by organophosphates causes homodimer dissociation through long-range allosteric effects. J Biol Chem :101007 (2021)
Blumenthal DK, Cheng X, Fajer M, Ho KY, Rohrer J, Gerlits O, Taylor P, Juneja P, Kovalevsky A, Radić Z

R_g^Guinier	3.9	nm
D_max	13.0	nm
Volume^Porod	162	nm³

SASDL92 – Human acetylcholinesterase, covalently bound to paraoxon

Sample:	Acetylcholinesterase dimer, 120 kDa Homo sapiens protein Acetylcholinesterase monomer, 60 kDa Homo sapiens protein
Buffer:	50 mM Tris/HCl, 100 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2015 May 29

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	142	nm³

SASDLZ2 – Immunoglobulin G3 (IgG3) Glycosylated

Immunoglobulin G subclass 3 experimental SAS data

Sample:	Immunoglobulin G subclass 3 monomer, 155 kDa Homo sapiens protein
Buffer:	20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 29

Solution structures of human myeloma IgG3 antibody reveal extended Fab and Fc regions relative to the other IgG subclasses. J Biol Chem :100995 (2021)
Spiteri VA, Goodall M, Doutch J, Rambo RP, Gor J, Perkins SJ

R_g^Guinier	7.0	nm
D_max	22.9	nm
Volume^Porod	267	nm³

SASDL23 – Immunoglobulin G3 (IgG3) Deglycosylated

Sample:	Immunoglobulin G subclass 3 monomer, 155 kDa Homo sapiens protein
Buffer:	20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 29

R_g^Guinier	7.1	nm
D_max	27.7	nm
Volume^Porod	294	nm³

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