Browse by ORGANISM: Homo sapiens (Human)

SASDKC4 – Complement factor P, properdin (FP) tetramer

Properdin (tetramer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (tetramer) tetramer, 220 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
Pedersen DV, Pedersen MN, Mazarakis SM, Wang Y, Lindorff-Larsen K, Arleth L, Andersen GR
RgGuinier 13.1 nm
Dmax 36.0 nm

SASDJK8 – N-terminal Angiopoietin-like protein 3 Hexamer

Angiopoietin-like protein 3 (N-terminal) experimental SAS data
DAMMIN model
Sample: Angiopoietin-like protein 3 (N-terminal) hexamer, 161 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.5, 400 mM NaCl, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12
Comparison of angiopoietin-like protein 3 and 4 reveals structural and mechanistic similarities. J Biol Chem :100312 (2021)
Gunn KH, Gutgsell AR, Xu Y, Johnson CV, Liu J, Neher SB
RgGuinier 5.6 nm
Dmax 34.0 nm
VolumePorod 380 nm3

SASDJL8 – N-terminal Angiopoietin-like protein 3 Trimer

Angiopoietin-related protein 3 experimental SAS data
DAMMIN model
Sample: Angiopoietin-related protein 3 trimer, 81 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.5, 400 mM NaCl, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12
Comparison of angiopoietin-like protein 3 and 4 reveals structural and mechanistic similarities. J Biol Chem :100312 (2021)
Gunn KH, Gutgsell AR, Xu Y, Johnson CV, Liu J, Neher SB
RgGuinier 4.6 nm
Dmax 21.5 nm
VolumePorod 71 nm3

SASDJM8 – N-terminal Angiopoietin-like protein 4 Trimer

Angiopoietin-related protein 4 experimental SAS data
DAMMIN model
Sample: Angiopoietin-related protein 4 trimer, 46 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl pH 7.4, 300 mM NaCl, 100 mM betaine, 500 mM arginine, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 8
Comparison of angiopoietin-like protein 3 and 4 reveals structural and mechanistic similarities. J Biol Chem :100312 (2021)
Gunn KH, Gutgsell AR, Xu Y, Johnson CV, Liu J, Neher SB
RgGuinier 4.7 nm
Dmax 16.0 nm
VolumePorod 69 nm3

SASDJ35 – Glucose-6-phosphate dehydrogenase dimer

Glucose-6-phosphate 1-dehydrogenase experimental SAS data
PYMOL model
Sample: Glucose-6-phosphate 1-dehydrogenase dimer, 119 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 Jul 24
Long-range structural defects by pathogenic mutations in most severe glucose-6-phosphate dehydrogenase deficiency Proceedings of the National Academy of Sciences 118(4) (2021)
Horikoshi N, Hwang S, Gati C, Matsui T, Castillo-Orellana C, Raub A, Garcia A, Jabbarpour F, Batyuk A, Broweleit J, Xiang X, Chiang A, Broweleit R, Vöhringer-Martinez E, Mochly-Rosen D, Wakatsuki S
RgGuinier 3.6 nm
Dmax 12.1 nm
VolumePorod 160 nm3

SASDJ45 – Glucose-6-phosphate dehydrogenase P396L mutant

Glucose-6-phosphate 1-dehydrogenase P396L experimental SAS data
CORAL model
Sample: Glucose-6-phosphate 1-dehydrogenase P396L dimer, 119 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 Jul 24
Long-range structural defects by pathogenic mutations in most severe glucose-6-phosphate dehydrogenase deficiency Proceedings of the National Academy of Sciences 118(4) (2021)
Horikoshi N, Hwang S, Gati C, Matsui T, Castillo-Orellana C, Raub A, Garcia A, Jabbarpour F, Batyuk A, Broweleit J, Xiang X, Chiang A, Broweleit R, Vöhringer-Martinez E, Mochly-Rosen D, Wakatsuki S
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 178 nm3

SASDHJ5 – Apolipoprotein-D plus biliverdin

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 168 nm3

SASDHK5 – Apolipoprotein-D oxidised with H2O2

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.3 nm
VolumePorod 164 nm3

SASDHL5 – Apolipoprotein-D plus palmitic acid

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.6 nm
VolumePorod 163 nm3

SASDHM5 – Apolipoprotein-D plus progesterone

Apolipoprotein D experimental SAS data
Apolipoprotein D Kratky plot
Sample: Apolipoprotein D tetramer, 77 kDa Homo sapiens protein
Buffer: 50 mM Na Phosphate, 150 mM NaCl, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 11
Small angle X-ray scattering analysis of ligand-bound forms of tetrameric apolipoprotein-D Bioscience Reports 41(1) (2021)
Kielkopf C, Whitten A, Garner B, Brown S
RgGuinier 3.3 nm
Dmax 10.0 nm
VolumePorod 161 nm3