Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDHW8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 5.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21

Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P

R_g^Guinier	4.9	nm
D_max	16.0	nm
Volume^Porod	274	nm³

SASDHX8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 6.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7

R_g^Guinier	4.6	nm
D_max	16.0	nm
Volume^Porod	258	nm³

SASDHY8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 7.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 26

R_g^Guinier	5.0	nm
D_max	17.4	nm
Volume^Porod	270	nm³

SASDHZ8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM TRIS, pH 8.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM TRIS, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Dec 15

R_g^Guinier	4.9	nm
D_max	16.7	nm
Volume^Porod	239	nm³

SASDH29 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM phosphate, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7

R_g^Guinier	4.9	nm
D_max	16.7	nm
Volume^Porod	240	nm³

SASDH65 – Poly(rC)-binding protein 2 (PCBP2)

Poly(rC)-binding protein 2 experimental SAS data

Sample:	Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein
Buffer:	5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 16

Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES. Nucleic Acids Res (2020)
Beckham SA, Matak MY, Belousoff MJ, Venugopal H, Shah N, Vankadari N, Elmlund H, Nguyen JHC, Semler BL, Wilce MCJ, Wilce JA

R_g^Guinier	3.3	nm
D_max	13.2	nm
Volume^Porod	76	nm³

SASDH75 – Poly(rC)-binding protein 2 (PCBP2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm) - PCBP2/SLIVm complex

Poly(rC)-binding protein 2modified stem loop IV poliovirus IRES, nucleotides 278-398 experimental SAS data

Poly(rC)-binding protein 2 modified stem loop IV poliovirus IRES, nucleotides 278-398 Kratky plot

Sample:	Poly(rC)-binding protein 2 monomer, 40 kDa Homo sapiens protein Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA
Buffer:	5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 16

R_g^Guinier	3.7	nm
D_max	11.5	nm
Volume^Porod	162	nm³

SASDH85 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH3) with bound modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398Truncated poly(rC)-binding protein 2 (ΔKH3)Truncated poly(rC)-binding protein 2 (ΔKH3) experimental SAS data

modified stem loop IV poliovirus IRES, nucleotides 278-398 Truncated poly(rC)-binding protein 2 (ΔKH3) Truncated poly(rC)-binding protein 2 (ΔKH3) Kratky plot

Sample:	Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Buffer:	5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 1

R_g^Guinier	3.8	nm
D_max	12.2	nm
Volume^Porod	165	nm³

SASDH95 – Truncated Poly(rC)-binding protein 2 (PCBP2-ΔKH3)

Truncated poly(rC)-binding protein 2 (ΔKH3) experimental SAS data

Truncated poly(rC)-binding protein 2 (ΔKH3) Kratky plot

Sample:	Truncated poly(rC)-binding protein 2 (ΔKH3) monomer, 28 kDa Homo sapiens protein
Buffer:	5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jan 1

R_g^Guinier	2.6	nm
D_max	9.2	nm
Volume^Porod	66	nm³

SASDHA5 – Truncated poly(rC)-binding protein 2 (PCBP2-ΔKH1-KH2) with bound modified RNA stem loop IV poliovirus IRES (SLIVm)

modified stem loop IV poliovirus IRES, nucleotides 278-398Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) experimental SAS data

modified stem loop IV poliovirus IRES, nucleotides 278-398 Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) Kratky plot

Sample:	Modified stem loop IV poliovirus IRES, nucleotides 278-398 monomer, 41 kDa Human poliovirus 1 … RNA Truncated poly(rC)-binding protein 2 (ΔKH1-KH2) monomer, 18 kDa Homo sapiens protein
Buffer:	5 mM HEPES-KOH, 25 mM KCl, 2 mM MgCl2, 2 mM DTT, 4 % glycerol, 0.1 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Jul 1

R_g^Guinier	3.5	nm
D_max	12.2	nm
Volume^Porod	126	nm³

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