Browse by ORGANISM: other species

SASDE84 – Tryparedoxin W70A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin W70A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W70A, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 7.2 nm
VolumePorod 42 nm3

SASDE94 – Tryparedoxin I109A, reduced state

Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 25 nm3

SASDEA4 – Tryparedoxin I109A, oxidized state

Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.7 nm
Dmax 4.3 nm
VolumePorod 27 nm3

SASDEB4 – Tryparedoxin I109A, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin I109A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin I109A, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 42 nm3

SASDEF4 – Tryparedoxin K102E, reduced state

Tryparedoxin K102E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin K102E monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 29 nm3

SASDEG4 – Tryparedoxin K102E, oxidized state

Tryparedoxin K102E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin K102E monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 4.3 nm
VolumePorod 26 nm3

SASDEH4 – Tryparedoxin K102E, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin K102E experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin K102E, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.8 nm
Dmax 6.2 nm
VolumePorod 37 nm3

SASDFJ3 – Maltose binding protein-SpoIVB peptidase fusion (MBP-SpoIVB)

SpoIVB peptidase (MBP fusion) experimental SAS data
CHIMERA model
Sample: SpoIVB peptidase (MBP fusion) monomer, 80 kDa Bacillus subtilis protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Jul 12
Solution Structure of SpoIVB Reveals Mechanism of PDZ Domain-Regulated Protease Activity. Front Microbiol 10:1232 (2019)
Xie X, Guo N, Xue G, Xie D, Yuan C, Harrison J, Li J, Jiang L, Huang M
RgGuinier 3.7 nm
Dmax 15.6 nm
VolumePorod 96 nm3

SASDEW7 – C-terminal truncated bank vole prion protein (amino acids 90-231)

Major prion protein experimental SAS data
C-terminal truncated bank vole prion protein (amino acids 90-231) Rg histogram
Sample: Major prion protein monomer, 16 kDa Myodes glareolus protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 25
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.3 nm
Dmax 9.4 nm
VolumePorod 29 nm3

SASDEX7 – C-terminal truncated bank vole prion protein (amino acids 90-231) bound to Cu(II)

Major prion protein experimental SAS data
C-terminal truncated bank vole prion protein (amino acids 90-231) bound to Cu(II) Rg histogram
Sample: Major prion protein monomer, 16 kDa Myodes glareolus protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 25
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.2 nm
Dmax 8.7 nm
VolumePorod 30 nm3