Small Angle Scattering Biological Data Bank SASBDB

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SASDM97 – Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv)

Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv) experimental SAS data

Sample:	Recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv) monomer, 29 kDa protein
Buffer:	phosphate buffered saline, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Oct 21

Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment. Arch Biochem Biophys 522(1):26-36 (2012)
Paoletti F, Malerba F, Konarev PV, Visintin M, Scardigli R, Fasulo L, Lamba D, Svergun DI, Cattaneo A

R_g^Guinier	2.7	nm
D_max	8.0	nm
Volume^Porod	39	nm³

SASDDK2 – Aspergillus fumigatus UDP galactopyranose mutase

Aspergillus fumigatus UDP galactopyranose mutase experimental SAS data

Sample:	Aspergillus fumigatus UDP galactopyranose mutase tetramer, 228 kDa protein
Buffer:	20 mM HEPES, 45 mM NaCl, 0.5 mM Tris(hydroxypropyl)phosphine, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Apr 19

Crystal structures and small-angle x-ray scattering analysis of UDP-galactopyranose mutase from the pathogenic fungus Aspergillus fumigatus. J Biol Chem 287(12):9041-51 (2012)
Dhatwalia R, Singh H, Oppenheimer M, Karr DB, Nix JC, Sobrado P, Tanner JJ

R_g^Guinier	4.7	nm
D_max	14.7	nm
Volume^Porod	308	nm³

SASDMJ9 – Plasmid pET11d- 229E710 encoding C-terminally His6-tagged HCoV-229E Nsp7-10 polyprotein

Replicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2

Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R

R_g^Guinier

2.1

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2

R_g^Guinier

3.0

SASDLG3 – Thermoplasma E2 catalytic core

Regulatory protein E2 experimental SAS data

Sample:	Regulatory protein E2, 1037 kDa Human papillomavirus type … protein
Buffer:	50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Jul 13

The catalytic core of an archaeal 2-oxoacid dehydrogenase multienzyme complex is a 42-mer protein assembly FEBS Journal 279(5):713-723 (2012)
Marrott N, Marshall J, Svergun D, Crennell S, Hough D, Danson M, van den Elsen J

R_g^Guinier	8.8	nm
D_max	22.0	nm
Volume^Porod	2473	nm³

SASDAA4 – Full length GbpA

Sample:	Full length GbpA monomer, 54 kDa Vibrio cholerae protein
Buffer:	25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 16

The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog 8(1):e1002373 (2012)
Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, Ibrahim AF, Svergun DI, Eijsink VG, Chatterjee NS, van Aalten DM

R_g^Guinier	3.9	nm
D_max	14.5	nm
Volume^Porod	100	nm³

SASDAB4 – Truncated GbpA

Sample:	Truncated GbpA monomer, 44 kDa Vibrio cholerae protein
Buffer:	25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 16

R_g^Guinier	3.6	nm
D_max	12.5	nm
Volume^Porod	80	nm³

SASDML9 – Structure of bifunctional protease-helicase NS3/4A domain assembly in solution

Genome polyprotein experimental SAS data

Sample:	Genome polyprotein dimer, 137 kDa Hepatitis C virus … protein
Buffer:	25 mM Tris, 1 M NaCl, 10% glycerol, 1 mM TCEP, 0.1% β-octyl glucoside, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 12

A macrocyclic HCV NS3/4A protease inhibitor interacts with protease and helicase residues in the complex with its full-length target Proceedings of the National Academy of Sciences 108(52):21052-21056 (2011)
Schiering N, D'Arcy A, Villard F, Simic O, Kamke M, Monnet G, Hassiepen U, Svergun D, Pulfer R, Eder J, Raman P, Bodendorf U

R_g^Guinier

3.9

SASDAF6 – K1K2K3 domain of Kgp gingipain

K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain experimental SAS data

Sample:	K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain monomer, 74 kDa Porphyromonas gingivalis W83 protein
Buffer:	10 mM TRIS 150 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at Anton Paar SAXSess, University of Sydney on 2010 Aug 15

The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis. Mol Microbiol 81(5):1358-73 (2011)
Li N, Yun P, Jeffries CM, Langley D, Gamsjaeger R, Church WB, Hunter N, Collyer CA

R_g^Guinier	4.3	nm
D_max	14.7	nm

SASDAG6 – K1K2 domains of Kgp gingipain

K1K2 adhesin modules of lysine-specific (Kgp) gingipain experimental SAS data

Sample:	K1K2 adhesin modules of lysine-specific (Kgp) gingipain monomer, 38 kDa Porphyromonas gingivalis W83 protein
Buffer:	10 mM TRIS 150 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at Anton Paar SAXSess, University of Sydney on 2010 Aug 15

R_g^Guinier	2.9	nm
D_max	9.5	nm

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