Small Angle Scattering Biological Data Bank SASBDB

SASDLD9 – 2-aminomuconic 6-semialdehyde dehydrogenase wild-type

2-aminomuconic 6-semialdehyde dehydrogenase experimental SAS data

Sample:	2-aminomuconic 6-semialdehyde dehydrogenase tetramer, 215 kDa Pseudomonas sp. protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1

The tetrameric assembly of 2-aminomuconic 6-semialdehyde dehydrogenase is a functional requirement of cofactor NAD(+) binding. Environ Microbiol 24(7):2994-3012 (2022)
Shi Q, Chen Y, Li X, Dong H, Chen C, Zhong Z, Yang C, Liu G, Su D

R_g^Guinier	3.9	nm
D_max	11.6	nm
Volume^Porod	326	nm³

SASDN77 – Truncated histone shaperone protein SET/TAF-Ib DC

SET nuclear proto-oncogene experimental SAS data

Sample:	SET nuclear proto-oncogene dimer, 53 kDa Homo sapiens protein
Buffer:	Sodium phosphate buffer, pH: 6.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 11

PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ Computational and Structural Biotechnology Journal (2022)
Casado-Combreras M, Rivero-Rodríguez F, Elena-Real C, Molodenskiy D, Díaz-Quintana A, Martinho M, Gerbaud G, González-Arzola K, Velázquez-Campoy A, Svergun D, Belle V, De la Rosa M, Díaz-Moreno I

R_g^Guinier	3.1	nm
D_max	9.7	nm
Volume^Porod	87	nm³

SASDN87 – Mixture 1:2 of truncated histone shaperone protein SET/TAF-Ib DC with cytochrome c

Cytochrome cSET nuclear proto-oncogene experimental SAS data

Sample:	Cytochrome c dimer, 24 kDa Homo sapiens protein SET nuclear proto-oncogene dimer, 53 kDa Homo sapiens protein
Buffer:	Sodium phosphate buffer, pH: 6.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 11

R_g^Guinier	4.2	nm
D_max	16.0	nm

SASDNC5 – Acinetobacter baumannii riboflavin biosynthesis protein RibD

Riboflavin biosynthesis protein RibD experimental SAS data

Sample:	Riboflavin biosynthesis protein RibD dimer, 80 kDa Acinetobacter baumannii protein
Buffer:	150 mM NaCl, 10 mM Tris, 1 mM DTT, 5% v/v glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
Weber K, Doellinger J, Jeffries CM, Wilharm G

R_g^Guinier	3.7	nm
D_max	12.0	nm
Volume^Porod	115	nm³

SASDND5 – Acinetobacter baumannii aconitate hydratase B, monomer fraction

Aconitate hydratase B experimental SAS data

Sample:	Aconitate hydratase B monomer, 96 kDa Acinetobacter baumannii protein
Buffer:	100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	150	nm³

SASDNE5 – Acinetobacter baumannii aconitate hydratase B, monomer/dimer mixed fraction

Sample:	Aconitate hydratase B , 96 kDa Acinetobacter baumannii protein
Buffer:	100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

R_g^Guinier	4.0	nm
D_max	14.0	nm
Volume^Porod	230	nm³

SASDNF5 – Acinetobacter baumannii transcriptional repressor NrdR (likely filamentous assembly)

Transcriptional repressor NrdR experimental SAS data

Transcriptional repressor NrdR Kratky plot

Sample:	Transcriptional repressor NrdR , 19 kDa Acinetobacter baumannii (strain … protein
Buffer:	100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

R_g^Guinier	19.9	nm
D_max	92.6	nm

SASDMB5 – Leucine-rich repeat protein SHOC-2

Leucine-rich repeat protein SHOC-2 experimental SAS data

Sample:	Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein
Buffer:	25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jun 20

Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J

R_g^Guinier	3.2	nm
D_max	11.2	nm
Volume^Porod	85	nm³

SASDMC5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C)

Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunit experimental SAS data

Sample:	Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20

Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J

R_g^Guinier	3.3	nm
D_max	11.7	nm
Volume^Porod	132	nm³

SASDMD5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) and GTPase KRas

Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunitGTPase KRas experimental SAS data

Sample:	Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein GTPase KRas monomer, 19 kDa Homo sapiens protein
Buffer:	25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20

Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J

R_g^Guinier	3.3	nm
D_max	11.7	nm
Volume^Porod	156	nm³

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