SASDD45 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein

Type II toxin-antitoxin system HicB family antitoxin experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 28
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 112 nm3

SASDD55 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein - bound to addiction module toxin, HicA

Type II toxin-antitoxin system HicB family antitoxinAddiction module toxin, HicA experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Addiction module toxin, HicA monomer, 7 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 27
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 110 nm3

SASDC75 – Leishmania braziliensis SGT co-chaperone

SGT protein experimental SAS data
DAMFILT model
Sample: SGT protein dimer, 92 kDa Leishmania braziliensis protein
Buffer: 20 mM Potassium Phosphate, 100 mM KCl, 10 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2014 May 14
Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinitie... Int J Biol Macromol 118(Pt A):693-706 (2018)
Coto ALS, Seraphim TV, Batista FAH, Dores-Silva PR, Barranco ABF, Teixeira FR, Gava LM, Borges JC
RgGuinier 4.5 nm
Dmax 17.0 nm

SASDJW2 – Modification dependent EcoKMcrA restriction endonuclease

5-methylcytosine-specific restriction enzyme A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 5-methylcytosine-specific restriction enzyme A dimer, 65 kDa Escherichia coli protein
Buffer: 20 mM Tris–HCl pH 7.5, 200 mM KCl, 0.1 mM EDTA, 0.01% (w/v) sodium azide and 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 May 24
Activity and structure of EcoKMcrA. Nucleic Acids Res 46(18):9829-9841 (2018)
Czapinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 95 nm3

SASDJX2 – Modification dependent EcoKMcrA restriction endonuclease in complex with cognate hemimethylated 12bp oligoduplex

cognate hemimethylated 12-bp oligoduplex5-methylcytosine-specific restriction enzyme A experimental SAS data
SASREF model
Sample: cognate hemimethylated 12-bp oligoduplex dimer, 15 kDa DNA
5-methylcytosine-specific restriction enzyme A dimer, 65 kDa Escherichia coli protein
Buffer: 20 mM Tris–HCl pH 7.5, 200 mM KCl, 0.1 mM EDTA, 0.01% (w/v) sodium azide and 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 May 24
Activity and structure of EcoKMcrA. Nucleic Acids Res 46(18):9829-9841 (2018)
Czapinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 3.9 nm
Dmax 13.5 nm
VolumePorod 69 nm3

SASDJY2 – N-terminal domain of modification dependent EcoKMcrA restriction endonuclease

5-methylcytosine-specific restriction enzyme A (N-terminal domain) experimental SAS data
OTHER model
Sample: 5-methylcytosine-specific restriction enzyme A (N-terminal domain) monomer, 21 kDa Escherichia coli protein
Buffer: 20 mM Tris–HCl pH 7.5, 200 mM KCl, 0.1 mM EDTA, 0.01% (w/v) sodium azide, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 May 24
Activity and structure of EcoKMcrA. Nucleic Acids Res 46(18):9829-9841 (2018)
Czapinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 2.1 nm
Dmax 7.5 nm
VolumePorod 29 nm3

SASDJZ2 – N-terminal domain of modification dependent EcoKMcrA restriction endonuclease in complex with cognate hemimethylated 12bp oligoduplex

5-methylcytosine-specific restriction enzyme A (N-terminal domain)cognate hemimethylated 12-bp oligoduplex experimental SAS data
OTHER model
Sample: 5-methylcytosine-specific restriction enzyme A (N-terminal domain) monomer, 21 kDa Escherichia coli protein
cognate hemimethylated 12-bp oligoduplex monomer, 7 kDa DNA
Buffer: 20 mM Tris–HCl pH 7.5, 200 mM KCl, 0.1 mM EDTA, 0.01% (w/v) sodium azide and 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 May 24
Activity and structure of EcoKMcrA. Nucleic Acids Res 46(18):9829-9841 (2018)
Czapinska H, Kowalska M, Zagorskaite E, Manakova E, Slyvka A, Xu SY, Siksnys V, Sasnauskas G, Bochtler M
RgGuinier 2.1 nm
Dmax 7.5 nm
VolumePorod 29 nm3

SASDEP7 – Filamin A Ig-like domains 3-5 P637Q mutant (FLNa3-5 P637Q)

Filamin A Ig.like domains 3-5 P637Q mutant experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Filamin A Ig.like domains 3-5 P637Q mutant monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Non-syndromic Mitral Valve Dysplasia Mutation Changes the Force Resilience and Interaction of Human Filamin A. Structure (2018)
Haataja TJK, Bernardi RC, Lecointe S, Capoulade R, Merot J, Pentikäinen U
RgGuinier 2.2 nm
Dmax 7.4 nm
VolumePorod 39 nm3

SASDEQ7 – Filamin A Ig-like domains 3-5 (FLNa3-5)

Filamin A Ig-like domains 3-5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Filamin A Ig-like domains 3-5 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Non-syndromic Mitral Valve Dysplasia Mutation Changes the Force Resilience and Interaction of Human Filamin A. Structure (2018)
Haataja TJK, Bernardi RC, Lecointe S, Capoulade R, Merot J, Pentikäinen U
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 39 nm3

SASDDL6 – Calcium Loaded Calbindin-D28K

Calbindin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Calbindin monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 3 mM CaCl2, pH: 7.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 28
The X-ray structure of human calbindin-D28K: an improved model. Acta Crystallogr D Struct Biol 74(Pt 10):1008-1014 (2018)
Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR
RgGuinier 2.1 nm
Dmax 7.3 nm
VolumePorod 47 nm3

4692 hits found.