Small Angle Scattering Biological Data Bank SASBDB


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32 hits found for Essen

SASDBF2 – Solution structure of 40bp long dsDNA-Sa Oligonucleotide

40bp long dsDNA-Sa Oligonucleotide experimental SAS data

Sample:	40bp long dsDNA-Sa Oligonucleotide monomer, 25 kDa DNA
Buffer:	0.5 x Tris/Borate/EDTA (TBE), pH:
Experiment:	SAXS data collected at BM29, ESRF on 2015 Aug 28

Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
...Essen LO, Peeters E, Albers SV

R_g^Guinier	3.6	nm
D_max	12.0	nm

SASDBG2 – Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).

Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). experimental SAS data

Sample:	Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer:	20 mM HEPES 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2015 Aug 27

R_g^Guinier	2.6	nm
D_max	9.8	nm
Volume^Porod	47	nm³

SASDBH2 – Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D

Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant experimental SAS data

Sample:	Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer:	20 mM HEPES 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2015 Aug 27

R_g^Guinier	2.6	nm
D_max	9.5	nm
Volume^Porod	51	nm³

SASDBJ2 – Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.

40bp long dsDNA-Sa OligonucleotideWild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). experimental SAS data

Sample:	40bp long dsDNA-Sa Oligonucleotide monomer, 25 kDa DNA Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer:	0.5 x Tris/Borate/EDTA (TBE), pH:
Experiment:	SAXS data collected at BM29, ESRF on 2015 Aug 27

R_g^Guinier	3.0	nm
D_max	11.8	nm

SASDUW2 – ArnA-ArnB complex, unphosphorylated

Conserved proteinConserved protein experimental SAS data

Sample:	Conserved protein monomer, 24 kDa Sulfolobus acidocaldarius (strain … protein Conserved protein monomer, 44 kDa Sulfolobus acidocaldarius (strain … protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 15

Sequential conformational transition of ArnB, an archaeal ortholog with Sec23/Sec24 core motif (2024)
Essen L, Korf L, Steinchen W, Watad M, Bezold F, Vogt M, Selbach L, Penner A, Tourte M, Hepp S, Albers S

R_g^Guinier	4.2	nm
D_max	19.2	nm
Volume^Porod	81	nm³

SASDDH3 – Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2)

Transcriptional regulator Lrs14-like protein experimental SAS data

Sample:	Transcriptional regulator Lrs14-like protein dimer, 33 kDa Sulfolobus acidocaldarius protein
Buffer:	300 mM NaCl, 20 mM HEPES, pH 7.5, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 May 5

Crystal structure of an Lrs14-like archaeal biofilm regulator from Sulfolobus acidocaldarius. Acta Crystallogr D Struct Biol 74(Pt 11):1105-1114 (2018)
...Essen LO, Banerjee A

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	57	nm³

SASDBX3 – Aureobox dsDNA

Sample:	Aureobox dsDNA monomer, 13 kDa synthetic construct DNA
Buffer:	50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
...Essen LO, Kottke T

R_g^Guinier	2.0	nm
D_max	8.6	nm
Volume^Porod	18	nm³

SASDBY3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE)

Aureochrome 1a bZIP-LOV module experimental SAS data

Sample:	Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer:	50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
...Essen LO, Kottke T

R_g^Guinier	3.4	nm
D_max	12.6	nm
Volume^Porod	115	nm³

SASDMY3 – Lysozyme amyloid fibril (LAF)

lysozyme amyloid fibril experimental SAS data

Sample:	lysozyme amyloid fibril , 1 kDa Gallus gallus protein
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

Dependence of the Nanoscale Composite Morphology of Fe3O4 Nanoparticle-Infused Lysozyme Amyloid Fibrils on Timing of Infusion: A Combined SAXS and AFM Study Molecules 26(16):4864 (2021)
Schroer M, Hu P, Tomasovicova N, Batkova M, Zakutanska K, Wu P, Kopcansky P

R_g^Guinier	38.4	nm
D_max	80.0	nm

SASDBZ3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage)/DNA complex (light state)

Aureobox dsDNAAureochrome 1a bZIP-LOV module experimental SAS data

Sample:	Aureobox dsDNA monomer, 13 kDa synthetic construct DNA Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer:	50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
...Essen LO, Kottke T

R_g^Guinier	4.5	nm
D_max	16.7	nm
Volume^Porod	97	nm³

SASDMZ3 – Fe3O4 nanoparticles (10 nm diameter)

Fe3O4 nanoparticles; nominal diameter 10 nm (hydrodynamic diameter) experimental SAS data

Fe3O4 nanoparticles; nominal diameter 10 nm (hydrodynamic diameter) Kratky plot

Sample:	Fe3O4 nanoparticles; nominal diameter 10 nm (hydrodynamic diameter) monomer, 1 kDa
Buffer:	50 mM borate buffer, 0.02% NaN3, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

R_g^Guinier	7.0	nm
D_max	8.0	nm

SASDB24 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state, Tris)

Aureochrome 1a bZIP-LOV module Kratky plot

Sample:	Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
...Essen LO, Kottke T

R_g^Guinier	3.9	nm
D_max	12.5	nm
Volume^Porod	121	nm³

SASDM24 – Fe3O4 nanoparticles (20 nm diameter)

Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) experimental SAS data

Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) Kratky plot

Sample:	Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) monomer, 1 kDa
Buffer:	50 mM borate buffer, 0.02% NaN3, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

R_g^Guinier	11.0	nm
D_max	14.0	nm

SASDB34 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (dark state, Tris)

Sample:	Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
...Essen LO, Kottke T

R_g^Guinier	3.8	nm
D_max	12.5	nm
Volume^Porod	117	nm³

SASDM34 – Fe3O4 nanoparticles (30 nm diameter)

Fe3O4 nanoparticles; nominal diameter 30 nm (hydrodynamic diameter) experimental SAS data

Fe3O4 nanoparticles; nominal diameter 30 nm (hydrodynamic diameter) Kratky plot

Sample:	Fe3O4 nanoparticles; nominal diameter 30 nm (hydrodynamic diameter) monomer, 1 kDa
Buffer:	50 mM borate buffer, 0.02% NaN3, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

R_g^Guinier	18.1	nm
D_max	16.9	nm

SASDM44 – LAF + P10 nanocomposite (NP added before fibrilization)

lysozyme amyloid fibrilFe3O4 nanoparticles; nominal diameter 10 nm (hydrodynamic diameter) experimental SAS data

Sample:	lysozyme amyloid fibril , 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 10 nm (hydrodynamic diameter) monomer, 1 kDa
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

R_g^Guinier	22.3	nm
D_max	70.0	nm

SASDM54 – LAF + P30 nanocomposite (NP added before fibrilization)

lysozyme amyloid fibrilFe3O4 nanoparticles; nominal diameter 30 nm (hydrodynamic diameter) experimental SAS data

Sample:	lysozyme amyloid fibril , 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 30 nm (hydrodynamic diameter) monomer, 1 kDa
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

R_g^Guinier	22.5	nm
D_max	95.0	nm

SASDM64 – LAF + P20 nanocomposite (NP added before fibrilization)

lysozyme amyloid fibrilFe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) experimental SAS data

Sample:	lysozyme amyloid fibril , 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) monomer, 1 kDa
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

R_g^Guinier	23.6	nm
D_max	90.0	nm

SASDM74 – LAF + P20 nanocomposite (NP added after fibrilization)

Sample:	lysozyme amyloid fibril , 1 kDa Gallus gallus protein Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) monomer, 1 kDa
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Sep 2

R_g^Guinier	31.0	nm
D_max	75.0	nm

SASDMJ4 – Lysozyme amyloid fibril (LAF)

Sample:	lysozyme amyloid fibril , 14 kDa Gallus gallus protein
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Sep 5

Effect of the concentration of protein and nanoparticles on the structure of biohybrid nanocomposites. Biopolymers 111(2):e23342 (2020)
Majorošová J, Schroer MA, Tomašovičová N, Batková M, Hu PS, Kubovčíková M, Svergun DI, Kopčanský P

R_g^Guinier	30.2	nm
D_max	120.0	nm

SASDMK4 – Fe3O4 nanoparticles (radius 5.6 nm )

Fe3O4 nanoparticles; radius 5.6 nm (AFM based) experimental SAS data

Fe3O4 nanoparticles; radius 5.6 nm (AFM based) Kratky plot

Sample:	Fe3O4 nanoparticles; radius 5.6 nm (AFM based) monomer, 1 kDa
Buffer:	water, HCLO4, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Sep 5

R_g^Guinier	11.0	nm
D_max	20.0	nm

SASDML4 – LAF + MNP (r = 5.6 nm) nanocomposite

lysozyme amyloid fibrilFe3O4 nanoparticles; radius 5.6 nm (AFM based) experimental SAS data

Sample:	lysozyme amyloid fibril , 1 kDa Gallus gallus protein Fe3O4 nanoparticles; radius 5.6 nm (AFM based) monomer, 1 kDa
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Sep 8

R_g^Guinier	29.4	nm
D_max	80.0	nm

SASDFS7 – Talin-1 head amino acids 1-405(Δ139-168)

Talin-1 (Δ139-168), human experimental SAS data

Talin-1 head amino acids 1-405(Δ139-168) Rg histogram

Sample:	Talin-1 (Δ139-168), human monomer, 48 kDa Homo sapiens protein
Buffer:	50 mM sodium phosphate, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24

The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering. J Cell Sci 133(19) (2020)
...Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Cheng RH, Wu J, Hytönen VP, Wehrle-Haller B

R_g^Guinier	3.3	nm
D_max	13.3	nm
Volume^Porod	77	nm³

SASDFT7 – Talin-1 head amino acids 1-405

Talin-1 head amino acids 1-405 Rg histogram

Sample:	Talin-1, human monomer, 51 kDa Homo sapiens protein
Buffer:	50 mM sodium phosphate, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24

R_g^Guinier	3.4	nm
D_max	11.5	nm
Volume^Porod	94	nm³

SASDFU7 – Talin-1 head amino acids 1-405(Δ134-170/GAG insert)

Talin-1 (Δ134-170/GAG insert), human experimental SAS data

Talin-1 head amino acids 1-405(Δ134-170/GAG insert) Rg histogram

Sample:	Talin-1 (Δ134-170/GAG insert), human monomer, 47 kDa Homo sapiens protein
Buffer:	50 mM sodium phosphate, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24

R_g^Guinier	3.0	nm
D_max	10.2	nm
Volume^Porod	71	nm³

SASDRK9 – single self-amplifying RNA

self-amplifying RNA experimental SAS data

Sample:	self-amplifying RNA monomer, 3030 kDa RNA
Buffer:	MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 10

Compact polyethylenimine-complexed mRNA vaccines Nature Nanotechnology (2025)
Moreno Herrero J, Stahl T, Erbar S, Maxeiner K, Schlegel A, Bacic T, Schumacher J, Cavalcanti L, Schroer M, Svergun D, Sahin U, Haas H

R_g^Guinier	90.5	nm
D_max	200.0	nm

SASDRL9 – single self-amplifying RNA + 50 mM NaCl

Sample:	self-amplifying RNA monomer, 3030 kDa RNA
Buffer:	MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 10

R_g^Guinier	30.7	nm
D_max	107.4	nm
Volume^Porod	112000	nm³

SASDRM9 – Polyethylenimine-compacted single self-amplifying RNA for prophylactic and therapeutic application, specifically for vaccination

self-amplifying RNAlinear polyethylenimine experimental SAS data

Sample:	self-amplifying RNA monomer, 3030 kDa RNA linear polyethylenimine monomer, 25 kDa none (polymer)
Buffer:	MBG buffer: 5% w/v D-Glucose, 10mM MES (2-(N-morpholino)ethanesulfonic acid) in double distillated sterile/RNAse free water, pH: 6.1
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 10

R_g^Guinier	12.0	nm
D_max	40.0	nm
Volume^Porod	9100	nm³

SASDAK8 – Dark state solution structure of Aureochrome1a- A´α-LOV-Jα

Aureochrome1a-A´α-LOV-Jα experimental SAS data

Sample:	Aureochrome1a-A´α-LOV-Jα dimer, 36 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2015 Mar 16

Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
...Essen LO

R_g^Guinier	3.2	nm
D_max	13.0	nm
Volume^Porod	67	nm³

SASDAJ8 – Light state solution structure of Aureochrome1a- A´α-LOV-Jα

Sample:	Aureochrome1a-A´α-LOV-Jα dimer, 36 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2015 Mar 16

Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
...Essen LO

R_g^Guinier	3.3	nm
D_max	13.0	nm
Volume^Porod	88	nm³

SASDBD2 – Dark state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

Aureochrome1a- A´α-LOV-Jα (Dark State) experimental SAS data

Sample:	Aureochrome1a- A´α-LOV-Jα (Dark State) dimer, 32 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2015 Sep 9

Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
...Essen LO

R_g^Guinier	2.7	nm
D_max	9.2	nm
Volume^Porod	58	nm³

SASDBE2 – Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

Aureochrome1a- A´α-LOV-Jα (Light State) experimental SAS data

Sample:	Aureochrome1a- A´α-LOV-Jα (Light State) dimer, 32 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2015 Sep 9

Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
...Essen LO

R_g^Guinier	2.6	nm
D_max	9.8	nm
Volume^Porod	66	nm³

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