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10 hits found for Jan Stransky

SASDKY7 – α-L-Fucosidase isoenzyme 2 from Paenibacillus thiaminolyticus - wild type

Alpha-L-fucosidase experimental SAS data
OTHER model
Sample: Alpha-L-fucosidase tetramer, 297 kDa Paenibacillus thiaminolyticus (Bacillus … protein
Buffer: 50 mM potassium phosphate, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Jul 16
The first structure–function study of GH151 α‐ l ‐fucosidase uncovers new oligomerization pattern, active site complementation, and selective substrate specificity The FEBS Journal (2022)
Koval'ová T, Kovaľ T, Stránský J, Kolenko P, Dušková J, Švecová L, Vodičková P, Spiwok V, Benešová E, Lipovová P, Dohnálek J
RgGuinier 4.2 nm
Dmax 13.2 nm
VolumePorod 418 nm3

SASDKZ7 – α-L-Fucosidase isoenzyme 2 from Paenibacillus thiaminolyticus - mutant H503A

Alpha-L-fucosidase H503A experimental SAS data
PYMOL model
Sample: Alpha-L-fucosidase H503A tetramer, 297 kDa Paenibacillus thiaminolyticus (Bacillus … protein
Buffer: 50 mM potassium phosphate, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Jul 16
The first structure–function study of GH151 α‐ l ‐fucosidase uncovers new oligomerization pattern, active site complementation, and selective substrate specificity The FEBS Journal (2022)
Koval'ová T, Kovaľ T, Stránský J, Kolenko P, Dušková J, Švecová L, Vodičková P, Spiwok V, Benešová E, Lipovová P, Dohnálek J
RgGuinier 4.5 nm
Dmax 13.5 nm
VolumePorod 433 nm3

SASDVL8 – Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated monomer

glucan 1,3-beta-glucosidase experimental SAS data
ALPHAFOLD model
Sample: glucan 1,3-beta-glucosidase monomer, 45 kDa Mucor circinelloides protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2020 Mar 26
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.3 nm
Dmax 6.4 nm
VolumePorod 53 nm3

SASDVM8 – Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated dimer

glucan 1,3-beta-glucosidase experimental SAS data
Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated dimer Rg histogram
Sample: glucan 1,3-beta-glucosidase dimer, 92 kDa Mucor circinelloides protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 27
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 140 nm3

SASDVN8 – Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated dimer

glucan 1,3-beta-glucosidase (short form, glycosylated) experimental SAS data
DAMMIN model
Sample: glucan 1,3-beta-glucosidase (short form, glycosylated) dimer, 89 kDa Mucor circinelloides protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 28
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 3.3 nm
Dmax 11.0 nm
VolumePorod 103 nm3

SASDVP8 – Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated tetramer

glucan 1,3-beta-glucosidase (long form, glycosylated) experimental SAS data
Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated tetramer Rg histogram
Sample: glucan 1,3-beta-glucosidase (long form, glycosylated) tetramer, 184 kDa Mucor circinelloides protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 28
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 4.8 nm
Dmax 15.1 nm
VolumePorod 171 nm3

SASDVQ8 – Glucosidase/Rutinosidase from Penicillium rubens - deglycosylated

glucan 1,3-beta-glucosidase experimental SAS data
ALPHAFOLD model
Sample: glucan 1,3-beta-glucosidase monomer, 42 kDa Penicillium rubens (strain … protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Nov 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.1 nm
Dmax 5.5 nm
VolumePorod 65 nm3

SASDVR8 – Glucosidase/Rutinosidase from Penicillium rubens - glycosylated

glucan 1,3-beta-glucosidase experimental SAS data
ALPHAFOLD model
Sample: glucan 1,3-beta-glucosidase monomer, 42 kDa Penicillium rubens (strain … protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Nov 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 68 nm3

SASDVS8 – Glucosidase/Rutinosidase from Aspergillus niger - deglycosylated

glucan 1,3-beta-glucosidase (W90L, N179H) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: glucan 1,3-beta-glucosidase (W90L, N179H) monomer, 42 kDa Aspergillus niger (strain … protein
Buffer: McIlvain (Na2HPO4/citric acid) buffer, pH: 3.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Jun 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 2.2 nm
Dmax 6.1 nm
VolumePorod 55 nm3

SASDVT8 – Glucosidase/Rutinosidase from Aspergillus niger - glycosylated

glucan 1,3-beta-glucosidase (W90L, N179H) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: glucan 1,3-beta-glucosidase (W90L, N179H) dimer, 83 kDa Aspergillus niger (strain … protein
Buffer: McIlvain (Na2HPO4/citric acid) buffer, pH: 3.5
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Jun 8
Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger
Jan Stransky
RgGuinier 3.0 nm
Dmax 8.1 nm
VolumePorod 111 nm3