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43 hits found for Jones

SASDHZ2 – 14-3-3 protein beta isoform

14-3-3 protein beta/alpha experimental SAS data
BUNCH model
Sample: 14-3-3 protein beta/alpha dimer, 58 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2011 Apr 7
The weak complex between RhoGAP protein ARHGAP22 and signal regulatory protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PLoS One 7(8):e41731 (2012)
...Jones A, Rowland AF, James DE, Martin JL
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 92 nm3

SASDH33 – Rho GTPase-activating protein 22

Rho GTPase-activating protein 22 experimental SAS data
BUNCH model
Sample: Rho GTPase-activating protein 22 monomer, 47 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2011 Apr 7
The weak complex between RhoGAP protein ARHGAP22 and signal regulatory protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PLoS One 7(8):e41731 (2012)
...Jones A, Rowland AF, James DE, Martin JL
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 88 nm3

SASDH83 – Chemically crossed linked complex between Rho GTPase-activating protein 22 (ARHGAP22) and the beta isoform of the 14-3-3 protein beta/alpha

14-3-3 protein beta/alphaRho GTPase-activating protein 22 experimental SAS data
CORAL model
Sample: 14-3-3 protein beta/alpha dimer, 58 kDa Homo sapiens protein
Rho GTPase-activating protein 22 monomer, 47 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2011 Apr 7
The weak complex between RhoGAP protein ARHGAP22 and signal regulatory protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PLoS One 7(8):e41731 (2012)
...Jones A, Rowland AF, James DE, Martin JL
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 195 nm3

SASDQT4 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.5 nm
Dmax 20.0 nm
VolumePorod 454 nm3

SASDQU4 – DNA-binding protein from starved cells: DgrDpsWT + 6 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.4 nm
Dmax 18.4 nm
VolumePorod 440 nm3

SASDQV4 – DNA-binding protein from starved cells: DgrDpsWT + 12 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.2 nm
Dmax 15.5 nm
VolumePorod 464 nm3

SASDQW4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.2 nm
Dmax 14.1 nm
VolumePorod 439 nm3

SASDQX4 – DNA-binding protein from starved cells: DgrDpsWT + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.2 nm
Dmax 14.4 nm
VolumePorod 427 nm3

SASDQY4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl, 5 mM EDTA

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS, 230 mM NaCl, 5 mM EDTA, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.5 nm
Dmax 19.5 nm
VolumePorod 453 nm3

SASDQZ4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.2 nm
Dmax 13.9 nm
VolumePorod 458 nm3

SASDQ25 – DNA-binding protein from starved cells: DgrDpsWT + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.4 nm
Dmax 19.3 nm
VolumePorod 438 nm3

SASDQ35 – DNA-binding protein from starved cells: DgrDps-D43A mutant in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.5 nm
Dmax 19.8 nm
VolumePorod 451 nm3

SASDQ45 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.4 nm
Dmax 19.1 nm
VolumePorod 412 nm3

SASDQ55 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.4 nm
Dmax 20.7 nm
VolumePorod 409 nm3

SASDQ65 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.5 nm
Dmax 20.6 nm
VolumePorod 404 nm3

SASDQ75 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8
Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
...Jones NC, Hoffmann SV, Pereira AS, Tavares P
RgGuinier 4.5 nm
Dmax 19.5 nm
VolumePorod 431 nm3

SASDPB5 – SAXS curve of RNA Xist A-repeats (Liu buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 25 mM K-HEPES, 0.1 mM Na-EDTA, 150 mM KCl, 15 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 12.1 nm
Dmax 45.0 nm

SASDPC5 – SAXS curve of RNA Xist A-repeats (Smola buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 100 mM HEPES, 100 mM NaCl, 10 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 9.8 nm
Dmax 40.0 nm
VolumePorod 657 nm3

SASDPD5 – SAXS curve of RNA Xist A-repeats (Maenner buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 20 mM HEPES-KOH, 100 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 20% glycerol, 3.25 mM MgCl2, pH: 7.9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 8.9 nm
Dmax 35.0 nm
VolumePorod 414 nm3

SASDPE5 – SAXS curve of RNA Xist A-repeats (Duszczyk buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 10 mM NaH2PO4/Na2HPO4, 100 mM NaCl, 0.02 mM EDTA, 0.02% azide, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 7.6 nm
Dmax 30.0 nm
VolumePorod 370 nm3

SASDPF5 – SAXS curve of RNA Xist A-repeats (14mer)

Xist A-repeat lncRNA 14 mer experimental SAS data
Xist A-repeat lncRNA 14 mer Kratky plot
Sample: Xist A-repeat lncRNA 14 mer monomer, 5 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 13 nm3

SASDPG5 – SAXS curve of RNA Xist A-repeats (26mer)

Xist A-repeat lncRNA 24 mer experimental SAS data
Xist A-repeat lncRNA 24 mer Kratky plot
Sample: Xist A-repeat lncRNA 24 mer dimer, 16 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 3.1 nm
Dmax 14.5 nm
VolumePorod 28 nm3

SASDPH5 – SAXS curve of RNA Xist A-repeats (1 repeat)

Xist A-repeat lncRNA 1 repeat experimental SAS data
Xist A-repeat lncRNA 1 repeat Kratky plot
Sample: Xist A-repeat lncRNA 1 repeat dimer, 41 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 3.9 nm
Dmax 16.0 nm
VolumePorod 42 nm3

SASDPJ5 – SAXS curve of RNA Xist A-repeats (2 repeats)

Xist A-repeat lncRNA 2 repeats experimental SAS data
Xist A-repeat lncRNA 2 repeats Kratky plot
Sample: Xist A-repeat lncRNA 2 repeats monomer, 34 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 87 nm3

SASDPK5 – SAXS curve of RNA Xist A-repeats (3 repeats)

Xist A-repeat lncRNA 3 repeats experimental SAS data
Xist A-repeat lncRNA 3 repeats Kratky plot
Sample: Xist A-repeat lncRNA 3 repeats monomer, 48 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 6.3 nm
Dmax 22.0 nm
VolumePorod 97 nm3

SASDPL5 – SAXS curve of RNA Xist A-repeats (4 repeats)

Xist A-repeat lncRNA 4 repeats experimental SAS data
Xist A-repeat lncRNA 4 repeats Kratky plot
Sample: Xist A-repeat lncRNA 4 repeats monomer, 62 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 7.2 nm
Dmax 34.0 nm

SASDPM5 – SAXS curve of RNA Xist A-repeats (5 repeats)

Xist A-repeat lncRNA 5 repeats experimental SAS data
Xist A-repeat lncRNA 5 repeats Kratky plot
Sample: Xist A-repeat lncRNA 5 repeats monomer, 81 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 7.7 nm
Dmax 30.0 nm
VolumePorod 186 nm3

SASDPN5 – SAXS curve of RNA Xist A-repeats (6 repeats)

Xist A-repeat lncRNA 6 repeats experimental SAS data
Xist A-repeat lncRNA 6 repeats Kratky plot
Sample: Xist A-repeat lncRNA 6 repeats monomer, 96 kDa RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 12.9 nm
Dmax 38.0 nm

SASDPP5 – SAXS curve of RNA Xist A-repeats (8 repeats)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 11.2 nm
Dmax 50.0 nm

SASDKT6 – SARS-CoV-2 non-structural protein 14 (nsp14)

Replicase polyprotein 1ab (non-structural protein 14) experimental SAS data
PYMOL model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
...Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A
RgGuinier 2.7 nm
Dmax 9.6 nm
VolumePorod 80 nm3

SASDKU6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
...Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A
RgGuinier 2.9 nm
Dmax 12.2 nm
VolumePorod 73 nm3

SASDKV6 – SARS-CoV-2 non-structural protein 10/non-structural protein 16 complex (nsp10/nsp16)

Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
...Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A
RgGuinier 2.0 nm
Dmax 8.0 nm
VolumePorod 28 nm3

SASDKW6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14/non-structural protein 16 triplex (nsp10/nsp14/nsp16)

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
...Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A
RgGuinier 4.7 nm
Dmax 11.4 nm
VolumePorod 113 nm3

SASDNY7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 50 mM NaCl pH 7.0

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22
The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
...Jones N, Hoffmann S, Tavares P, Pereira A
RgGuinier 4.4 nm
Dmax 16.1 nm
VolumePorod 409 nm3

SASDNZ7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 80 mM NaCl pH 7.0

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 80 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22
The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
...Jones N, Hoffmann S, Tavares P, Pereira A
RgGuinier 4.5 nm
Dmax 17.1 nm
VolumePorod 475 nm3

SASDN28 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 230 mM NaCl pH 7.0

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22
The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
...Jones N, Hoffmann S, Tavares P, Pereira A
RgGuinier 4.5 nm
Dmax 20.6 nm
VolumePorod 438 nm3

SASDN38 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 480 mM NaCl pH 7.0

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data
GASBOR model
Sample: DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 480 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22
The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
...Jones N, Hoffmann S, Tavares P, Pereira A
RgGuinier 4.8 nm
Dmax 20.5 nm
VolumePorod 430 nm3

SASDN48 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 50 mM NaCl pH 7.0

DNA protection during starvation, DPS-∆N (Ferritin superfamily) experimental SAS data
DAMMIN model
Sample: DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22
The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
...Jones N, Hoffmann S, Tavares P, Pereira A
RgGuinier 3.8 nm
Dmax 9.3 nm
VolumePorod 290 nm3

SASDN58 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 230 mM NaCl pH 7.0

DNA protection during starvation, DPS-∆N (Ferritin superfamily) experimental SAS data
DAMMIN model
Sample: DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer: 50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22
The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
...Jones N, Hoffmann S, Tavares P, Pereira A
RgGuinier 3.8 nm
Dmax 8.8 nm
VolumePorod 291 nm3

SASDH79 – Human Cation-independent mannose-6-phosphate receptor domains 9-10

Cation-independent mannose-6-phosphate receptor experimental SAS data
MULTIFOXS model
Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Jones E, Berger I, Hassan A, Crump M
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 93 nm3

SASDH59 – Human Cation-independent mannose-6-phosphate receptor domains 9-10 wih mannose-6-phosphate

Cation-independent mannose-6-phosphate receptor experimental SAS data
OTHER model
Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Jones E, Berger I, Hassan A, Crump M
RgGuinier 3.3 nm
Dmax 9.1 nm
VolumePorod 88 nm3

SASDH69 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated

Cation-independent mannose-6-phosphate receptor experimental SAS data
OTHER model
Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Jones E, Berger I, Hassan A, Crump M
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 48 nm3

SASDJ23 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated with mannose-6-phosphate

Cation-independent mannose-6-phosphate receptor experimental SAS data
MULTIFOXS model
Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Jones E, Berger I, Hassan A, Crump M
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 69 nm3