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9 hits found for Pietras

SASDP34 – Ubiquitin-conjugating enzyme E2 conjugated to deuterated Ubiquitin (SANS data at 0% v/v D2O, plus additional SANS with contrast variation and SAXS datasets)

Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S)Ubiquitin experimental SAS data
MONSA model
Sample: Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S) monomer, 17 kDa Homo sapiens protein
Ubiquitin monomer, 9 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2019 May 24
Production and characterisation of modularly deuterated UBE2D1–Ub conjugate by small angle neutron and X-ray scattering European Biophysics Journal (2022)
Pietras Z, Duff A, Morad V, Wood K, Jeffries C, Sunnerhagen M
RgGuinier 2.1 nm
Dmax 7.4 nm

SASDWE4 – The nonstructural 2A protein of the human parechovirus 1 (HPeV1) short form, amino acids 1-129

Genome polyprotein (Protein 2A H-NC; Δ130-150) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Genome polyprotein (Protein 2A H-NC; Δ130-150) , 15 kDa Human parechovirus 1 … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM DDT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 28
Structural plasticity of 2A proteins in the Parechovirus family
Zuzanna Pietras
RgGuinier 2.0 nm
Dmax 9.5 nm
VolumePorod 43 nm3

SASDXU7 – N-Myc proto-oncogene protein residues 1-69

N-myc proto-oncogene protein, residues 1-69 experimental SAS data
N-myc proto-oncogene protein, residues 1-69 Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-69 monomer, 8 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A. Nat Commun (2026)
...Pietras Z, Khare LP, Derbyshire D, Resetca D, Arrowsmith CH, Aili D, Ekström S, Penn LZ, Wallner B, Ahlner A, Sunnerhagen M
RgGuinier 2.4 nm
Dmax 11.0 nm
VolumePorod 12 nm3

SASDXV7 – N-Myc proto-oncogene protein, residues 1-100

N-myc proto-oncogene protein, residues 1-100 experimental SAS data
N-myc proto-oncogene protein, residues 1-100 Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-100 monomer, 11 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A. Nat Commun (2026)
...Pietras Z, Khare LP, Derbyshire D, Resetca D, Arrowsmith CH, Aili D, Ekström S, Penn LZ, Wallner B, Ahlner A, Sunnerhagen M
RgGuinier 3.0 nm
Dmax 13.0 nm
VolumePorod 22 nm3

SASDXW7 – Kinase domain of Aurora kinase A mutant C290A:C393A

Aurora kinase A mutant C290A:C393A experimental SAS data
Aurora kinase A mutant C290A:C393A Kratky plot
Sample: Aurora kinase A mutant C290A:C393A monomer, 33 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A. Nat Commun (2026)
...Pietras Z, Khare LP, Derbyshire D, Resetca D, Arrowsmith CH, Aili D, Ekström S, Penn LZ, Wallner B, Ahlner A, Sunnerhagen M
RgGuinier 2.2 nm
Dmax 9.5 nm
VolumePorod 59 nm3

SASDXX7 – N-Myc proto-oncogene residues 1-69 in complex with Aurora kinase A mutant C290A:C393A

N-myc proto-oncogene protein, residues 1-69Aurora kinase A mutant C290A:C393A experimental SAS data
N-myc proto-oncogene protein, residues 1-69 Aurora kinase A mutant C290A:C393A Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-69 monomer, 8 kDa Homo sapiens protein
Aurora kinase A mutant C290A:C393A monomer, 33 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Nov 28
The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A. Nat Commun (2026)
...Pietras Z, Khare LP, Derbyshire D, Resetca D, Arrowsmith CH, Aili D, Ekström S, Penn LZ, Wallner B, Ahlner A, Sunnerhagen M
RgGuinier 2.5 nm
Dmax 10.2 nm
VolumePorod 62 nm3

SASDXY7 – N-Myc proto-oncogene residues 1-100 in complex with Aurora kinase A mutant C290A:C393A

N-myc proto-oncogene protein, residues 1-100Aurora kinase A mutant C290A:C393A experimental SAS data
N-myc proto-oncogene protein, residues 1-100 Aurora kinase A mutant C290A:C393A Kratky plot
Sample: N-myc proto-oncogene protein, residues 1-100 monomer, 11 kDa Homo sapiens protein
Aurora kinase A mutant C290A:C393A monomer, 33 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 5mM MgCl2, 3% v/v glycerol, 2mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 9
The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A. Nat Commun (2026)
...Pietras Z, Khare LP, Derbyshire D, Resetca D, Arrowsmith CH, Aili D, Ekström S, Penn LZ, Wallner B, Ahlner A, Sunnerhagen M
RgGuinier 2.8 nm
Dmax 11.4 nm
VolumePorod 82 nm3

SASDMG9 – Multidrug resistance operon repressor (MexR) of the MexAB-OprM multidrug efflux pump operon of Pseudomonas aeruginosa

Multidrug resistance operon repressor experimental SAS data
DAMFILT model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
Buffer: 20mM HEPES, 150mM NaCl, 10mM DTT, 1% v/v glycerol, pH: 7.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Nov 23
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
...Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 56 nm3

SASDMH9 – Pseudomonas aeruginosa Multidrug resistance operon repressor (MexR) in complex with 34bp dsDNA binding sequence (SANS data at 0% D2O)

Multidrug resistance operon repressor34 base pair double-stranded DNA experimental SAS data
MONSA model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
34 base pair double-stranded DNA monomer, 21 kDa synthetic construct DNA
Buffer: 20mM NaPO4, 150 mM NaCl, 10 mM DTT, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 May 30
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
...Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.9 nm
Dmax 7.8 nm
VolumePorod 79 nm3