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18
hits found for
Valentini
SASDA46
– TIP5 in Tris
Sample:
Bromodomain adjacent to zinc finger domain protein 2A monomer, 27 kDa
Homo sapiens
protein
Buffer:
20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Mar 16
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Structure
23(1):80-92 (2015)
...
Valentini
E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
R
g
Guinier
3.0
nm
D
max
10.0
nm
Volume
Porod
45
nm
3
SASDA56
– BAZ2B in Tris
Sample:
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa
Homo sapiens
protein
Buffer:
20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 24
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Structure
23(1):80-92 (2015)
...
Valentini
E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
R
g
Guinier
4.0
nm
D
max
14.5
nm
Volume
Porod
43
nm
3
SASDA66
– BAZ2B-18mer complex in Tris
Sample:
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa
Homo sapiens
protein
H3Kac9Kac14 monomer, 5 kDa
Homo sapiens
protein
Buffer:
20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 24
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Structure
23(1):80-92 (2015)
...
Valentini
E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
R
g
Guinier
4.2
nm
D
max
16.0
nm
Volume
Porod
52
nm
3
SASDAK6
– Glucose Isomerase
Sample:
Xylose isomerase tetramer, 172 kDa
Streptomyces rubiginosus
protein
Buffer:
PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Apr 24
Standard proteins
Erica
Valentini
R
g
Guinier
3.4
nm
D
max
9.7
nm
Volume
Porod
293
nm
3
SASDAP7
– PBX1-PREB1 complex
Sample:
Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa
Homo sapiens
protein
Homeobox protein PKNOX1 monomer, 38 kDa
Homo sapiens
protein
Buffer:
20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Dec 6
The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
FEBS J
283(16):3134-54 (2016)
...
Valentini
E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
R
g
Guinier
5.8
nm
D
max
19.0
nm
Volume
Porod
143
nm
3
SASDAQ7
– PBX1-PREB1-DNA complex
Sample:
Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa
Homo sapiens
protein
Homeobox protein PKNOX1 monomer, 38 kDa
Homo sapiens
protein
DNA 22mer monomer, 12 kDa DNA
Buffer:
20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Dec 6
The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
FEBS J
283(16):3134-54 (2016)
...
Valentini
E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
R
g
Guinier
4.8
nm
D
max
16.3
nm
Volume
Porod
173
nm
3
SASDAR7
– DNA 22mer
Sample:
DNA 22mer monomer, 12 kDa DNA
Buffer:
20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Dec 6
The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
FEBS J
283(16):3134-54 (2016)
...
Valentini
E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
R
g
Guinier
2.2
nm
D
max
7.5
nm
Volume
Porod
19
nm
3
SASDAT7
– Aptamer AIR-3 5FU
Sample:
AIR3-5FU dimer, 69 kDa RNA
Buffer:
water, pH: 7.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Oct 31
Structure and target interaction of a G-quadruplex RNA-aptamer.
RNA Biol
13(10):973-987 (2016)
...
Valentini
E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
R
g
Guinier
4.3
nm
D
max
16.0
nm
Volume
Porod
77
nm
3
SASDAU7
– Aptamer AIR-3 FdU
Sample:
AIR3-FdU dimer, 69 kDa RNA
Buffer:
water, pH: 7.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Oct 31
Structure and target interaction of a G-quadruplex RNA-aptamer.
RNA Biol
13(10):973-987 (2016)
...
Valentini
E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
R
g
Guinier
5.0
nm
D
max
16.0
nm
Volume
Porod
67
nm
3
SASDAV7
– Aptamer AIR-3A
Sample:
AIR-3A dimer, 13 kDa RNA
Buffer:
water, pH: 7.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Dec 6
Structure and target interaction of a G-quadruplex RNA-aptamer.
RNA Biol
13(10):973-987 (2016)
...
Valentini
E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
R
g
Guinier
1.9
nm
D
max
6.5
nm
Volume
Porod
14
nm
3
SASDAW7
– Aptamer AIR-3A 2'FU
Sample:
AIR-3A 2'FU dimer, 13 kDa RNA
Buffer:
water, pH: 7.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Dec 6
Structure and target interaction of a G-quadruplex RNA-aptamer.
RNA Biol
13(10):973-987 (2016)
...
Valentini
E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
R
g
Guinier
1.9
nm
D
max
6.0
nm
Volume
Porod
12
nm
3
SASDAX7
– Aptamer AIR-3A G18U
Sample:
AIR-3A G18U tetramer, 25 kDa RNA
Buffer:
water, pH: 7.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2013 Dec 6
Structure and target interaction of a G-quadruplex RNA-aptamer.
RNA Biol
13(10):973-987 (2016)
...
Valentini
E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
R
g
Guinier
2.5
nm
D
max
8.3
nm
Volume
Porod
24
nm
3
SASDAY7
– IL-6R tetramer-dimer equilibrium
Sample:
Interleukin-6 receptor subunit alpha tetramer, 164 kDa
Homo sapiens
protein
Interleukin-6 receptor subunit alpha dimer, 82 kDa
Homo sapiens
protein
Buffer:
water, pH: 7.5
Experiment:
SAXS data collected at B21, Diamond Light Source
on 2014 Oct 1
Structure and target interaction of a G-quadruplex RNA-aptamer.
RNA Biol
13(10):973-987 (2016)
...
Valentini
E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
R
g
Guinier
4.9
nm
D
max
20.0
nm
Volume
Porod
240
nm
3
SASDAZ7
– IL-6R AIR-3A 2:4 complex
Sample:
AIR-3A tetramer, 26 kDa RNA
Interleukin-6 receptor subunit alpha dimer, 82 kDa
Homo sapiens
protein
Buffer:
water, pH: 7.5
Experiment:
SAXS data collected at B21, Diamond Light Source
on 2014 Oct 1
Structure and target interaction of a G-quadruplex RNA-aptamer.
RNA Biol
13(10):973-987 (2016)
...
Valentini
E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
R
g
Guinier
6.7
nm
D
max
25.0
nm
Volume
Porod
150
nm
3
SASDA88
– RAID3 in PBS
Sample:
RAID3 dimer, 23 kDa RNA
Buffer:
PBS 137 mM NaCl 2,7 mM KCl 6,5 mM Na2HPO4 1,5 mM KH2PO4, pH: 7.4
Experiment:
SAXS data collected at B21, Diamond Light Source
on 2013 Oct 31
RAID3--An interleukin-6 receptor-binding aptamer with post-selective modification-resistant affinity.
RNA Biol
12(9):1043-53 (2015)
...
Valentini
E, Svergun DI, Berg K, Lorenzen I, Grötzinger J, Rose-John S, Hahn U
R
g
Guinier
2.7
nm
D
max
10.0
nm
Volume
Porod
26
nm
3
SASDKV9
– Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(48bp)
Sample:
Upstream stimulatory factor 1 dimer, 50 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit alpha monomer, 10 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit beta monomer, 11 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit gamma monomer, 11 kDa
Homo sapiens
protein
DNA 48bp monomer, 30 kDa DNA
Buffer:
100 mM cacodylate buffer, pH: 6.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2015 Jun 14
The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding
International Journal of Biological Macromolecules
(2021)
...
Valentini
E, Svergun D, Nardini M, Mantovani R, Gnesutta N
R
g
Guinier
4.8
nm
D
max
17.0
nm
Volume
Porod
155
nm
3
SASDKW9
– Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(50bp)
Sample:
Upstream stimulatory factor 1 dimer, 50 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit alpha monomer, 10 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit beta monomer, 11 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit gamma monomer, 11 kDa
Homo sapiens
protein
DNA 50bp monomer, 31 kDa DNA
Buffer:
100 mM cacodylate buffer, pH: 6.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2015 Jun 14
The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding
International Journal of Biological Macromolecules
(2021)
...
Valentini
E, Svergun D, Nardini M, Mantovani R, Gnesutta N
R
g
Guinier
4.8
nm
D
max
17.0
nm
Volume
Porod
165
nm
3
SASDKX9
– Transcription factors MAX and NF-Y bound to a CCAAT DNA box(48bp)
Sample:
Protein max (Isoform 2, short, 13-21: missing) dimer, 39 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit alpha monomer, 10 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit beta monomer, 11 kDa
Homo sapiens
protein
Nuclear transcription factor Y subunit gamma monomer, 11 kDa
Homo sapiens
protein
DNA 48bp monomer, 30 kDa DNA
Buffer:
100 mM cacodylate buffer, pH: 6.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2012 Dec 10
The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding
International Journal of Biological Macromolecules
(2021)
...
Valentini
E, Svergun D, Nardini M, Mantovani R, Gnesutta N
R
g
Guinier
5.2
nm
D
max
17.0
nm
Volume
Porod
151
nm
3