Small Angle Scattering Biological Data Bank SASBDB


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18 hits found for Valentini

SASDA46 – TIP5 in Tris

Bromodomain adjacent to zinc finger domain protein 2A experimental SAS data

Sample:	Bromodomain adjacent to zinc finger domain protein 2A monomer, 27 kDa Homo sapiens protein
Buffer:	20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Mar 16

Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC. Structure 23(1):80-92 (2015)
...Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A

R_g^Guinier	3.0	nm
D_max	10.0	nm
Volume^Porod	45	nm³

SASDA56 – BAZ2B in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminal experimental SAS data

Sample:	Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa Homo sapiens protein
Buffer:	20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 24

R_g^Guinier	4.0	nm
D_max	14.5	nm
Volume^Porod	43	nm³

SASDA66 – BAZ2B-18mer complex in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminalH3Kac9Kac14 experimental SAS data

Bromodomain adjacent to zinc finger domain protein 2B, C-terminal H3Kac9Kac14 Kratky plot

Sample:	Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa Homo sapiens protein H3Kac9Kac14 monomer, 5 kDa Homo sapiens protein
Buffer:	20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 24

R_g^Guinier	4.2	nm
D_max	16.0	nm
Volume^Porod	52	nm³

SASDAK6 – Glucose Isomerase

Sample:	Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer:	PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 24

Standard proteins
Erica Valentini

R_g^Guinier	3.4	nm
D_max	9.7	nm
Volume^Porod	293	nm³

SASDAP7 – PBX1-PREB1 complex

Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1 experimental SAS data

Sample:	Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding. FEBS J 283(16):3134-54 (2016)
...Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C

R_g^Guinier	5.8	nm
D_max	19.0	nm
Volume^Porod	143	nm³

SASDAQ7 – PBX1-PREB1-DNA complex

Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1DNA 22mer experimental SAS data

Sample:	Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein DNA 22mer monomer, 12 kDa DNA
Buffer:	20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

R_g^Guinier	4.8	nm
D_max	16.3	nm
Volume^Porod	173	nm³

SASDAR7 – DNA 22mer

Sample:	DNA 22mer monomer, 12 kDa DNA
Buffer:	20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

R_g^Guinier	2.2	nm
D_max	7.5	nm
Volume^Porod	19	nm³

SASDAT7 – Aptamer AIR-3 5FU

Sample:	AIR3-5FU dimer, 69 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
...Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U

R_g^Guinier	4.3	nm
D_max	16.0	nm
Volume^Porod	77	nm³

SASDAU7 – Aptamer AIR-3 FdU

Sample:	AIR3-FdU dimer, 69 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	5.0	nm
D_max	16.0	nm
Volume^Porod	67	nm³

SASDAV7 – Aptamer AIR-3A

Sample:	AIR-3A dimer, 13 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

R_g^Guinier	1.9	nm
D_max	6.5	nm
Volume^Porod	14	nm³

SASDAW7 – Aptamer AIR-3A 2'FU

Sample:	AIR-3A 2'FU dimer, 13 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

R_g^Guinier	1.9	nm
D_max	6.0	nm
Volume^Porod	12	nm³

SASDAX7 – Aptamer AIR-3A G18U

Sample:	AIR-3A G18U tetramer, 25 kDa RNA
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

R_g^Guinier	2.5	nm
D_max	8.3	nm
Volume^Porod	24	nm³

SASDAY7 – IL-6R tetramer-dimer equilibrium

Interleukin-6 receptor subunit alphaInterleukin-6 receptor subunit alpha experimental SAS data

Sample:	Interleukin-6 receptor subunit alpha tetramer, 164 kDa Homo sapiens protein Interleukin-6 receptor subunit alpha dimer, 82 kDa Homo sapiens protein
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2014 Oct 1

R_g^Guinier	4.9	nm
D_max	20.0	nm
Volume^Porod	240	nm³

SASDAZ7 – IL-6R AIR-3A 2:4 complex

AIR-3AInterleukin-6 receptor subunit alpha experimental SAS data

Sample:	AIR-3A tetramer, 26 kDa RNA Interleukin-6 receptor subunit alpha dimer, 82 kDa Homo sapiens protein
Buffer:	water, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2014 Oct 1

R_g^Guinier	6.7	nm
D_max	25.0	nm
Volume^Porod	150	nm³

SASDA88 – RAID3 in PBS

Sample:	RAID3 dimer, 23 kDa RNA
Buffer:	PBS 137 mM NaCl 2,7 mM KCl 6,5 mM Na2HPO4 1,5 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2013 Oct 31

RAID3--An interleukin-6 receptor-binding aptamer with post-selective modification-resistant affinity. RNA Biol 12(9):1043-53 (2015)
...Valentini E, Svergun DI, Berg K, Lorenzen I, Grötzinger J, Rose-John S, Hahn U

R_g^Guinier	2.7	nm
D_max	10.0	nm
Volume^Porod	26	nm³

SASDKV9 – Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(48bp)

Upstream stimulatory factor 1Nuclear transcription factor Y subunit alphaNuclear transcription factor Y subunit betaNuclear transcription factor Y subunit gammaDNA 48bp experimental SAS data

Sample:	Upstream stimulatory factor 1 dimer, 50 kDa Homo sapiens protein Nuclear transcription factor Y subunit alpha monomer, 10 kDa Homo sapiens protein Nuclear transcription factor Y subunit beta monomer, 11 kDa Homo sapiens protein Nuclear transcription factor Y subunit gamma monomer, 11 kDa Homo sapiens protein DNA 48bp monomer, 30 kDa DNA
Buffer:	100 mM cacodylate buffer, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 14

The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding International Journal of Biological Macromolecules (2021)
...Valentini E, Svergun D, Nardini M, Mantovani R, Gnesutta N

R_g^Guinier	4.8	nm
D_max	17.0	nm
Volume^Porod	155	nm³

SASDKW9 – Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(50bp)

Sample:	Upstream stimulatory factor 1 dimer, 50 kDa Homo sapiens protein Nuclear transcription factor Y subunit alpha monomer, 10 kDa Homo sapiens protein Nuclear transcription factor Y subunit beta monomer, 11 kDa Homo sapiens protein Nuclear transcription factor Y subunit gamma monomer, 11 kDa Homo sapiens protein DNA 50bp monomer, 31 kDa DNA
Buffer:	100 mM cacodylate buffer, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 14

R_g^Guinier	4.8	nm
D_max	17.0	nm
Volume^Porod	165	nm³

SASDKX9 – Transcription factors MAX and NF-Y bound to a CCAAT DNA box(48bp)

Protein max (Isoform 2, short, 13-21: missing)Nuclear transcription factor Y subunit alphaNuclear transcription factor Y subunit betaNuclear transcription factor Y subunit gammaDNA 48bp experimental SAS data

Sample:	Protein max (Isoform 2, short, 13-21: missing) dimer, 39 kDa Homo sapiens protein Nuclear transcription factor Y subunit alpha monomer, 10 kDa Homo sapiens protein Nuclear transcription factor Y subunit beta monomer, 11 kDa Homo sapiens protein Nuclear transcription factor Y subunit gamma monomer, 11 kDa Homo sapiens protein DNA 48bp monomer, 30 kDa DNA
Buffer:	100 mM cacodylate buffer, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Dec 10

R_g^Guinier	5.2	nm
D_max	17.0	nm
Volume^Porod	151	nm³

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