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51 hits found for Cordeiro

SASDE32 – Mothers against decapentaplegic homolog 5 - Smad5 MH1

Mothers against decapentaplegic homolog 5 experimental SAS data
Mothers against decapentaplegic homolog 5 Kratky plot
Sample: Mothers against decapentaplegic homolog 5 , 15 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 14
Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes (2019)
...Cordeiro T, Pluta R, Macias M
RgGuinier 1.9 nm
Dmax 6.6 nm
VolumePorod 32 nm3

SASDE42 – Mothers against decapentaplegic homolog 8_9 - Smad8_9 MH1

Mothers against decapentaplegic homolog 8_9 experimental SAS data
Mothers against decapentaplegic homolog 8_9 Kratky plot
Sample: Mothers against decapentaplegic homolog 8_9 , 15 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 1
Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes (2019)
...Cordeiro T, Pluta R, Macias M
RgGuinier 1.9 nm
Dmax 6.5 nm
VolumePorod 34 nm3

SASDLA2 – Mothers against decapentaplegic homolog 2, SMAD2 phosphomimetic mutant 0.5 mg/ml

Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) experimental SAS data
Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) , 53 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 4
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 5.1 nm
Dmax 20.0 nm

SASDLB2 – Mothers against decapentaplegic homolog 2, SMAD2 phosphomimetic mutant 1 mg/ml

Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) experimental SAS data
Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) , 53 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 4
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 5.2 nm
Dmax 20.0 nm

SASDLC2 – Mothers against decapentaplegic homolog 2, SMAD2 phosphomimetic mutant 1.5 mg/ml

Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) experimental SAS data
Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (C-terminus phosphomimetic mutant) , 53 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 4
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 5.3 nm
Dmax 20.0 nm

SASDF34 – Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) experimental SAS data
Sample: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 20
Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Structure (2019)
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P
RgGuinier 4.7 nm
Dmax 17.7 nm
VolumePorod 102 nm3

SASDF44 – RXR/RAR Heterodimer : N-CoRNID Complex (1:1)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD)Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) experimental SAS data
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) Kratky plot
Sample: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein
Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein
Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) monomer, 28 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Jul 23
Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Structure (2019)
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P
RgGuinier 4.8 nm
Dmax 19.4 nm
VolumePorod 167 nm3

SASDF54 – RXRΔH12/RAR Heterodimer : N-CoRNID Complex (1:1)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD)Retinoid-X receptor alpha (RXR-alpha) Δ helix12 experimental SAS data
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) Retinoid-X receptor alpha (RXR-alpha) Δ helix12 Kratky plot
Sample: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein
Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein
Retinoid-X receptor alpha (RXR-alpha) Δ helix12 monomer, 24 kDa Mus musculus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Jul 23
Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Structure (2019)
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P
RgGuinier 4.2 nm
Dmax 15.7 nm
VolumePorod 183 nm3

SASDF64 – RXR/RAR Heterodimer : N-CoRNID Complex (1:1) with RAR inverse agonist (BMS493)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD)Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) experimental SAS data
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) Kratky plot
Sample: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein
Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein
Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) monomer, 28 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Jul 23
Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Structure (2019)
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P
RgGuinier 4.8 nm
Dmax 19.5 nm
VolumePorod 178 nm3

SASDF74 – RXR/RARI396E Heterodimer : N-CoRNID Complex (1:1)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD)Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) mutant I396E experimental SAS data
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) mutant I396E Kratky plot
Sample: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein
Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein
Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) mutant I396E monomer, 28 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 9
Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Structure (2019)
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P
RgGuinier 5.3 nm
Dmax 22.4 nm
VolumePorod 171 nm3

SASDF84 – RXR/RAR Heterodimer : N-CoRNID Complex (1:1) with RAR agonist (Am580)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD)Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) experimental SAS data
Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) Kratky plot
Sample: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein
Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein
Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) monomer, 28 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Jul 23
Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Structure (2019)
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P
RgGuinier 4.2 nm
Dmax 17.2 nm
VolumePorod 131 nm3

SASDC95 – Light encoded DNA biosensor: e14A DNA

e14A experimental SAS data
DAMMIF model
Sample: e14A monomer, 21 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 16
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 2.8 nm
Dmax 12.5 nm

SASDCA5 – Light encoded DNA biosensor: e14B DNA

e14B experimental SAS data
DAMMIF model
Sample: e14B monomer, 13 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 16
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 1.9 nm
Dmax 9.0 nm

SASDCB5 – Light encoded DNA biosensor: e14C DNA

e14C experimental SAS data
DAMFILT model
Sample: e14C monomer, 5 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 16
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 1.3 nm
Dmax 5.0 nm

SASDCC5 – Light encoded DNA biosensor: e14AB DNA

e14AB experimental SAS data
DAMFILT model
Sample: e14AB monomer, 93 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 16
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 5.4 nm
Dmax 24.7 nm

SASDCD5 – Light encoded DNA biosensor: e14ABC DNA

e14ABC experimental SAS data
DAMFILT model
Sample: e14ABC monomer, 106 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 16
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 4.7 nm
Dmax 25.0 nm
VolumePorod 152 nm3

SASDCE5 – Light encoded DNA biosensor: e13A DNA

e13A experimental SAS data
DAMFILT model
Sample: e13A monomer, 17 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDCF5 – Light encoded DNA biosensor: e13B DNA

e13B experimental SAS data
DAMFILT model
Sample: e13B monomer, 10 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 1.8 nm
Dmax 7.0 nm
VolumePorod 13 nm3

SASDCG5 – Light encoded DNA biosensor: e13C DNA

e13C experimental SAS data
DAMFILT model
Sample: e13C monomer, 6 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 1.3 nm
Dmax 5.0 nm
VolumePorod 8 nm3

SASDCH5 – Light encoded DNA biosensor: e13AB DNA

e13AB experimental SAS data
DAMFILT model
Sample: e13AB monomer, 79 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 4.7 nm
Dmax 20.0 nm
VolumePorod 103 nm3

SASDCJ5 – Light encoded DNA biosensor: e13ABC DNA

e13ABC experimental SAS data
DAMFILT model
Sample: e13ABC monomer, 42 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 3.9 nm
Dmax 18.0 nm
VolumePorod 59 nm3

SASDCK5 – Light encoded DNA biosensor: e13Ae14Be13C DNA

e13Ae14Be13C experimental SAS data
DAMFILT model
Sample: e13Ae14Be13C monomer, 40 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 16
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 3.7 nm
Dmax 18.0 nm
VolumePorod 65 nm3

SASDCL5 – Light encoded DNA biosensor: e14Ae13Be14C DNA

e14Ae13Be14C experimental SAS data
DAMFILT model
Sample: e14Ae13Be14C monomer, 31 kDa DNA
Buffer: 154 mM NaCl, pH: 8.3
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 16
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor. ACS Chem Biol 13(5):1235-1242 (2018)
Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
RgGuinier 3.4 nm
Dmax 14.0 nm
VolumePorod 42 nm3

SASDJ28 – Ferric Iron Reductase - FhuF∆1-17

Ferric iron reductase protein FhuF (∆1-17) experimental SAS data
DAMFILT model
Sample: Ferric iron reductase protein FhuF (∆1-17) monomer, 28 kDa Escherichia coli (strain … protein
Buffer: 20 mM Phosphate, 200 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 16
Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli JBIC Journal of Biological Inorganic Chemistry (2021)
...Cordeiro T, Piccioli M, Louro R
RgGuinier 2.1 nm
Dmax 8.8 nm
VolumePorod 60 nm3

SASDHL8 – Aquifex aeolicus McoA metaloxidase evolved variant (2F4)

McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) experimental SAS data
OTHER model
Sample: McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) monomer, 55 kDa Aquifex aeolicus VF5 protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 25
Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase ACS Catalysis :5022-5035 (2022)
...Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 6.8 nm
VolumePorod 78 nm3

SASDHM8 – Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352)

Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant  (2F4∆328-352) experimental SAS data
DAMMIF model
Sample: Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352) monomer, 53 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 18
Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase ACS Catalysis :5022-5035 (2022)
...Cordeiro T, Martins L
RgGuinier 2.2 nm
Dmax 6.6 nm
VolumePorod 74 nm3

SASDKZ8 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 0.74 mg/ml

Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2 , 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 3.4 nm
Dmax 15.0 nm

SASDK29 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 1.3 mg/ml

Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2 , 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 3.8 nm
Dmax 21.0 nm

SASDK39 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 2.5 mg/ml

Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2 , 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 3.8 nm
Dmax 20.0 nm

SASDK49 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 3mg/ml

Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2 , 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 4.3 nm
Dmax 23.0 nm

SASDK59 – Mothers against decapentaplegic homolog 2, SMAD2, linker fragment (amino acids 175-262)

Mothers against decapentaplegic homolog 2 (linker) experimental SAS data
Mothers against decapentaplegic homolog 2 (linker) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (linker) monomer, 10 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 2.9 nm
Dmax 14.0 nm
VolumePorod 20 nm3

SASDK69 – Mothers against decapentaplegic homolog 2, SMAD2, linker-MH2 fragment (amino acids 186-467) 0.5 mg/ml

Mothers against decapentaplegic homolog 2 (linker-MH2) experimental SAS data
Mothers against decapentaplegic homolog 2 (linker-MH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (linker-MH2) , 99 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 3.6 nm
Dmax 15.0 nm

SASDK79 – Mothers against decapentaplegic homolog 2, SMAD2, linker-MH2 fragment (amino acids 186-467) 1.1 mg/ml

Mothers against decapentaplegic homolog 2 (linker-MH2) experimental SAS data
Mothers against decapentaplegic homolog 2 (linker-MH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (linker-MH2) , 99 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 3.7 nm
Dmax 17.0 nm

SASDK89 – Mothers against decapentaplegic homolog 2, SMAD2, linker-MH2 fragment (amino acids 186-467) 2.2 mg/ml

Mothers against decapentaplegic homolog 2 (linker-MH2) experimental SAS data
Mothers against decapentaplegic homolog 2 (linker-MH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (linker-MH2) , 99 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 4.2 nm
Dmax 19.0 nm

SASDK99 – Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 1 mg/ml

Mothers against decapentaplegic homolog 2 (C-terminus deletion) experimental SAS data
Mothers against decapentaplegic homolog 2 (C-terminus deletion) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (C-terminus deletion) , 158 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 4.1 nm
Dmax 17.0 nm

SASDKA9 – Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 2 mg/ml

Mothers against decapentaplegic homolog 2 (C-terminus deletion) experimental SAS data
Mothers against decapentaplegic homolog 2 (C-terminus deletion) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (C-terminus deletion) , 158 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 4.3 nm
Dmax 20.0 nm

SASDKB9 – Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 3.8 mg/ml

Mothers against decapentaplegic homolog 2 (C-terminus deletion) experimental SAS data
Mothers against decapentaplegic homolog 2 (C-terminus deletion) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (C-terminus deletion) , 158 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 4.6 nm
Dmax 22.0 nm

SASDKC9 – Mothers against decapentaplegic homolog 4, SMAD4, wild-type

Mothers against decapentaplegic homolog 4 experimental SAS data
Mothers against decapentaplegic homolog 4 Kratky plot
Sample: Mothers against decapentaplegic homolog 4 monomer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 4
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 4.4 nm
Dmax 22.0 nm

SASDBD9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

ABC transporter periplasmic substrate-binding protein experimental SAS data
SREFLEX model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
...Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 51 nm3

SASDKD9 – Mothers against decapentaplegic homolog 4, SMAD4, linker fragment (amino acids 141-271)

Mothers against decapentaplegic homolog 4 (linker) experimental SAS data
Mothers against decapentaplegic homolog 4 (linker) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (linker) monomer, 14 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 3.7 nm
Dmax 17.5 nm
VolumePorod 45 nm3

SASDBE9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

ABC transporter periplasmic substrate-binding protein experimental SAS data
SREFLEX model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
...Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 49 nm3

SASDKE9 – Mothers against decapentaplegic homolog 4, SMAD4, linker-MH2 fragment (amino acids 150-552)

Mothers against decapentaplegic homolog 4 (linker-MH2) experimental SAS data
Mothers against decapentaplegic homolog 4 (linker-MH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (linker-MH2) monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 3.3 nm
Dmax 17.0 nm
VolumePorod 80 nm3

SASDBF9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of tungstate

ABC transporter periplasmic substrate-binding protein experimental SAS data
BUNCH model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
...Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 46 nm3

SASDKF9 – Mothers against decapentaplegic homolog 4, SMAD4, SADMH2 fragment (amino acids 272-552)

Mothers against decapentaplegic homolog 4 (SADMH2) experimental SAS data
Mothers against decapentaplegic homolog 4 (SADMH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (SADMH2) monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 54 nm3

SASDBG9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of tungstate

ABC transporter periplasmic substrate-binding protein experimental SAS data
BUNCH model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
...Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.3 nm
Dmax 8.9 nm
VolumePorod 44 nm3

SASDKG9 – Mothers against decapentaplegic homolog 4, SMAD4, MH2 fragment (amino acids 314-552)

Mothers against decapentaplegic homolog 4 (MH2 Fragment) experimental SAS data
Mothers against decapentaplegic homolog 4 (MH2 Fragment) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (MH2 Fragment) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 2.1 nm
Dmax 8.0 nm

SASDBH9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of molybdate

ABC transporter periplasmic substrate-binding protein experimental SAS data
BUNCH model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
...Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.3 nm
Dmax 9.2 nm
VolumePorod 46 nm3

SASDKH9 – Mothers against decapentaplegic homolog 4, SMAD4, MH1 fragment (amino acids 10-140)

Mothers against decapentaplegic homolog 4 (MH1 fragment) experimental SAS data
Mothers against decapentaplegic homolog 4 (MH1 fragment) Kratky plot
Sample: Mothers against decapentaplegic homolog 4 (MH1 fragment) monomer, 15 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 20
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
...Cordeiro T, Macias M
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 24 nm3

SASDFY7 – Aquifex aeolicus McoA metaloxidase

Aquifex aeolicus McoA metaloxidase experimental SAS data
DAMFILT model
Sample: Aquifex aeolicus McoA metaloxidase monomer, 55 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 15
The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
...Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 79 nm3

SASDFX7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆337-346 (MCoA∆337-346)

Aquifex aeolicus McoA metaloxidase ∆337-346 experimental SAS data
DAMMIF model
Sample: Aquifex aeolicus McoA metaloxidase ∆337-346 monomer, 54 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 4
The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
...Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 7.0 nm
VolumePorod 78 nm3

SASDFW7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆328-352 (MCoA∆328-352)

Aquifex aeolicus McoA metaloxidase ∆328-352  (MCoA∆328-352) experimental SAS data
DAMFILT model
Sample: Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) monomer, 53 kDa Aquifex aeolicus protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 13
The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
...Cordeiro T, Martins L
RgGuinier 2.3 nm
Dmax 6.9 nm
VolumePorod 77 nm3