SASBDB entries for UniProt ID:

SASDHH4 – N-terminal domain of Wag31 from M. tuberculosis

UniProt ID: P9WMU1 (2-60) Cell wall synthesis protein Wag31
Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 tetramer, 34 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 3
Structural basis of self-assembly in the lipid-binding domain of mycobacterial polar growth factor Wag31 IUCrJ 7(4):767-776 (2020)
Choukate K, Chaudhuri B
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 52 nm3

SASDHJ4 – Dengue Virus 2 New Guinea C (DENV-2 C, capsid protein), apo form

UniProt ID: P14340 (21-100) Dengue Virus 2 New Guinea C
Dengue Virus 2 New Guinea C experimental SAS data
CORAL model
Sample: Dengue Virus 2 New Guinea C dimer, 19 kDa Dengue virus 2 protein
Buffer: 100 mM NaCl, 25 mM HEPES, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2018 Jul 5
A cocrystal structure of dengue capsid protein in complex of inhibitor. Proc Natl Acad Sci U S A 117(30):17992-18001 (2020)
Xia H, Xie X, Zou J, Noble CG, Russell WK, Holthauzen LMF, Choi KH, White MA, Shi PY
RgGuinier 1.8 nm
Dmax 5.4 nm
VolumePorod 52 nm3

SASDHK4 – Dengue Virus 2 New Guinea C (DENV-2 C, capsid protein) with saturating ST148 inhibitor

UniProt ID: P14340 (21-100) Dengue Virus 2 New Guinea C
Dengue Virus 2 New Guinea C experimental SAS data
CORAL model
Sample: Dengue Virus 2 New Guinea C dimer, 19 kDa Dengue virus 2 protein
Buffer: 100 mM NaCl, 25 mM HEPES, pH 7.4, 10 µM ST148, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2018 Jul 26
A cocrystal structure of dengue capsid protein in complex of inhibitor. Proc Natl Acad Sci U S A 117(30):17992-18001 (2020)
Xia H, Xie X, Zou J, Noble CG, Russell WK, Holthauzen LMF, Choi KH, White MA, Shi PY
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 52 nm3

SASDHL4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR)

UniProt ID: P11717 (36-761) Cation-independent mannose-6-phosphate receptor
Cation-independent mannose-6-phosphate receptor experimental SAS data
Cation-independent mannose-6-phosphate receptor Kratky plot
Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Oct 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 3.7 nm
Dmax 10.1 nm
VolumePorod 140 nm3

SASDHM4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR) from SEC-SAXS

UniProt ID: P11717 (36-761) Cation-independent mannose-6-phosphate receptor
Cation-independent mannose-6-phosphate receptor experimental SAS data
Cation-independent mannose-6-phosphate receptor Kratky plot
Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 3.7 nm
Dmax 13.5 nm
VolumePorod 147 nm3

SASDHN4 – N-terminal 5 domains of the cation-independent mannose-6-phosphate receptor (CI-MPR) bound to mannose 6-phosphate (M6P)

UniProt ID: P11717 (36-761) Cation-independent mannose-6-phosphate receptor
Cation-independent mannose-6-phosphate receptor experimental SAS data
Cation-independent mannose-6-phosphate receptor Kratky plot
Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 3.7 nm
Dmax 13.3 nm
VolumePorod 150 nm3

SASDHP4 – Palmitoyl-protein thioesterase 1 (PPT1)

UniProt ID: P50897 (28-306) Palmitoyl-protein thioesterase 1
Palmitoyl-protein thioesterase 1 experimental SAS data
Palmitoyl-protein thioesterase 1 Kratky plot
Sample: Palmitoyl-protein thioesterase 1 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 2.3 nm
Dmax 9.8 nm
VolumePorod 54 nm3

SASDHQ4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR) in complex with palmitoyl-protein thioesterase 1 (PPT1)

UniProt ID: P11717 (36-761) Cation-independent mannose-6-phosphate receptor

UniProt ID: P50897 (28-306) Palmitoyl-protein thioesterase 1
Cation-independent mannose-6-phosphate receptorPalmitoyl-protein thioesterase 1 experimental SAS data
Cation-independent mannose-6-phosphate receptor Palmitoyl-protein thioesterase 1 Kratky plot
Sample: Cation-independent mannose-6-phosphate receptor monomer, 81 kDa Homo sapiens protein
Palmitoyl-protein thioesterase 1 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH: 6.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Nov 12
Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor. Commun Biol 3(1):498 (2020)
Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM
RgGuinier 4.9 nm
Dmax 19.3 nm
VolumePorod 258 nm3

SASDHR4 – A structural characterization of poly(aspartic acid) hydrolase-1 from Sphingomonas sp. KT-1

UniProt ID: Q7WSC1 (41-314) Poly(Aspartic acid) hydrolase-1
Poly(Aspartic acid) hydrolase-1 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Poly(Aspartic acid) hydrolase-1 dimer, 65 kDa Sphingomonas sp. KT-1 protein
Buffer: 20 mM Tris pH 7.0, 100 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27
Structural Characterization of Sphingomonas sp. KT-1 PahZ1-Catalyzed Biodegradation of Thermally Synthesized Poly(aspartic acid) ACS Sustainable Chemistry & Engineering (2020)
Brambley C, Bolay A, Salvo H, Jansch A, Yared T, Miller J, Wallen J, Weiland M
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 66 nm3

SASDHS4 – Protein ninH from Bacteriophage lambda (N-terminal histidine tagged) T15A mutated

UniProt ID: P03771 (1-68) Protein ninH
Protein ninH experimental SAS data
MODELLER model
Sample: Protein ninH dimer, 20 kDa Escherichia phage lambda protein
Buffer: 150 mM NaCl, 50 mM phosphate buffer (pH 7.4), 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss 2.0 with MetalJet, Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University on 2019 Jul 1
A bacteriophage mimic of the bacterial nucleoid-associated protein Fis. Biochem J (2020)
Chakraborti S, Balakrishnan D, Trotter AJ, Gittens WH, Yang AWH, Jolma A, Paterson JR, Świątek S, Plewka J, Curtis FA, Bowers LY, Pålsson LO, Hughes TR, Taube M, Kozak M, Heddle JG, Sharples GJ
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 33 nm3