Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDJX5 – Solution Scattering of Zeitlupe G46S:G80R

UniProt ID: Q94BT6 (29-190) Adagio protein 1 (Zeitlupe G46S:G80R)

Adagio protein 1 (Zeitlupe G46S:G80R) experimental SAS data

Sample:	Adagio protein 1 (Zeitlupe G46S:G80R) dimer, 37 kDa Arabidopsis thaliana protein
Buffer:	50 mM HEPES, 100 mM NaCl, 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 2

Steric and Electronic Interactions at Gln154 in ZEITLUPE Induce Reorganization of the LOV Domain Dimer Interface. Biochemistry (2020)
Pudasaini A, Green R, Song YH, Blumenfeld A, Karki N, Imaizumi T, Zoltowski BD

R_g^Guinier	2.2	nm
D_max	8.4	nm
Volume^Porod	41	nm³

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

UniProt ID: P50098 (1-512) Inosine-5'-monophosphate dehydrogenase

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

UniProt ID: P50098 (1-512) Inosine-5'-monophosphate dehydrogenase

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJ26 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-monomer

UniProt ID: P36056 (17-628) Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data

Sample:	Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial monomer, 70 kDa Saccharomyces cerevisiae protein
Buffer:	40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 May 1

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam

R_g^Guinier	3.8	nm
D_max	13.9	nm
Volume^Porod	160	nm³

SASDJ36 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer

UniProt ID: P36056 (17-628) dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial

dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data

Sample:	Dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein
Buffer:	40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 May 1

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam

R_g^Guinier	5.0	nm
D_max	18.2	nm
Volume^Porod	516	nm³

SASDJ46 – Fep1-rec-GATA

UniProt ID: A4VAR6 (1-208) GATA-type iron responsive transcription factor Fep1 reconstituted

UniProt ID: None (None-None) 24-mer double strand DNA from the GATA promoter

GATA-type iron responsive transcription factor Fep1 reconstituted24-mer double strand DNA from the GATA promoter experimental SAS data

GATA-type iron responsive transcription factor Fep1 reconstituted 24-mer double strand DNA from the GATA promoter Kratky plot

Sample:	GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. Eur Biophys J (2021)
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC

R_g^Guinier	3.5	nm
D_max	13.1	nm
Volume^Porod	73	nm³

SASDJ56 – Fep1-GATA

UniProt ID: A4VAR6 (1-208) GATA-type iron responsive transcription factor Fep1

UniProt ID: None (None-None) 24-mer double strand DNA from the GATA promoter

GATA-type iron responsive transcription factor Fep124-mer double strand DNA from the GATA promoter experimental SAS data

GATA-type iron responsive transcription factor Fep1 24-mer double strand DNA from the GATA promoter Kratky plot

Sample:	GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein 24-mer double strand DNA from the GATA promoter dimer, 14 kDa synthetic oligonucleotide DNA
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	85	nm³

SASDJ76 – Fep1 wild type with [2Fe2S] cluster reconstituted

UniProt ID: A4VAR6 (1-208) GATA-type iron responsive transcription factor Fep1 reconstituted

GATA-type iron responsive transcription factor Fep1 reconstituted experimental SAS data

Fep1 wild type with [2Fe2S] cluster reconstituted Rg histogram

Sample:	GATA-type iron responsive transcription factor Fep1 reconstituted monomer, 22 kDa Komagataella pastoris protein
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

R_g^Guinier	3.8	nm
D_max	16.5	nm
Volume^Porod	53	nm³

SASDJ86 – Fep1 wild type

UniProt ID: A4VAR6 (1-208) GATA-type iron responsive transcription factor Fep1

GATA-type iron responsive transcription factor Fep1 experimental SAS data

Sample:	GATA-type iron responsive transcription factor Fep1 monomer, 22 kDa Komagataella pastoris protein
Buffer:	50 mM MOPS, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 5

R_g^Guinier	3.5	nm
D_max	11.8	nm
Volume^Porod	41	nm³

SASDJ96 – Trinucleosomes from Xenopus laevis (African clawed frog)

UniProt ID: Q92133 (None-None) Histone H3

UniProt ID: P62799 (None-None) Histone H4

UniProt ID: P06897 (None-None) Histone H2A type 1

UniProt ID: Q92130 (None-None) Histone H2B

UniProt ID: None (None-None) Non-linker Ended Trinucleosome DNA

Histone H3Histone H4Histone H2A type 1Histone H2BNon-linker Ended Trinucleosome DNA experimental SAS data

Histone H3 Histone H4 Histone H2A type 1 Histone H2B Non-linker Ended Trinucleosome DNA Kratky plot

Sample:	Histone H3 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B monomer, 14 kDa Xenopus laevis protein Non-linker Ended Trinucleosome DNA monomer, 172 kDa DNA
Buffer:	20 mM Tris 150 mM NaCl 1 mM EDTA 1 mM DTT 50% w/v sucrose, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Mar 11

Solution structure(s) of trinucleosomes from contrast variation SAXS Nucleic Acids Research (2021)
Mauney A, Muthurajan U, Luger K, Pollack L

R_g^Guinier	12.9	nm
D_max	41.8	nm

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