SASBDB entries for UniProt ID:

SASDLQ8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Compact

UniProt ID: Q9UK39 (None-None) Nocturnin - Deletion construct - Δ107-120
Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Compact Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 80 nm3

SASDLR8 – Human apo Nocturnin - N terminus truncated (118-NOCT)

UniProt ID: Q9UK39 (118-431) Nocturnin - N terminus truncated (118-NOCT)
Nocturnin - N terminus truncated (118-NOCT) experimental SAS data
EOM/RANCH model
Sample: Nocturnin - N terminus truncated (118-NOCT) monomer, 36 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
Wickramaratne AC, Li L, Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.3 nm
Dmax 10.3 nm
VolumePorod 63 nm3

SASDLY8 – Mycobacterium tuberculosis Rv0792c, a GntR homolog

UniProt ID: O86331 (None-None) Recombinant Mycobacterium tuberculosis H37Rv
Recombinant Mycobacterium tuberculosis H37Rv experimental SAS data
OTHER model
Sample: Recombinant Mycobacterium tuberculosis H37Rv dimer, 65 kDa Mycobacterium tuberculosis H37Rv protein
Buffer: 25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 2
Structural and Functional Characterization of Rv0792c from Mycobacterium tuberculosis: Identifying Small Molecule Inhibitor against HutC Protein. Microbiol Spectr :e0197322 (2022)
Chauhan NK, Anand A, Sharma A, Dhiman K, Gosain TP, Singh P, Singh P, Khan E, Chattopadhyay G, Kumar A, Sharma D, Ashish, Sharma TK, Singh R
RgGuinier 3.2 nm
Dmax 12.0 nm

SASDL29 – SAXS data from RW4-MPC at 1 mg/ml at 283 K

UniProt ID: A0A0N1BQH2 (1-311) Metapyrocatechase
Metapyrocatechase experimental SAS data
MODELLER model
Sample: Metapyrocatechase tetramer, 139 kDa Novosphingobium sp. AAP93 protein
Buffer: 20 mM Tris-HCl 250 mM NaCl, pH 8.0, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Dec 20
Shape-function of a novel metapyrocatechase, RW4-MPC: Metagenomics to SAXS data based insight into deciphering regulators of function. Int J Biol Macromol 188:1012-1024 (2021)
Vasudeva G, Sidhu C, Kalidas N, Ashish, Pinnaka AK
RgGuinier 3.7 nm
Dmax 10.2 nm

SASDL39 – Autophagy-related protein 23

UniProt ID: Q06671 (1-453) Autophagy-related protein 23
Autophagy-related protein 23 experimental SAS data
Autophagy-related protein 23 Kratky plot
Sample: Autophagy-related protein 23 dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 3
Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ
RgGuinier 7.4 nm
Dmax 29.3 nm
VolumePorod 378 nm3

SASDL49 – Autophagy-related protein 23 L171A,I182A,L189A mutant

UniProt ID: Q06671 (1-453) Autophagy-related protein 23 LIL Mutant
Autophagy-related protein 23 LIL Mutant experimental SAS data
Autophagy-related protein 23 LIL Mutant Kratky plot
Sample: Autophagy-related protein 23 LIL Mutant monomer, 52 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ
RgGuinier 4.4 nm
Dmax 17.1 nm
VolumePorod 98 nm3

SASDL59 – Autophagy-related protein 23 R190A,K193A,K194A mutant

UniProt ID: Q06671 (1-453) Autophagy-related protein 23 RKK Mutant
Autophagy-related protein 23 RKK Mutant experimental SAS data
Autophagy-related protein 23 RKK Mutant Kratky plot
Sample: Autophagy-related protein 23 RKK Mutant dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
Hawkins WD, Leary KA, Andhare D, Popelka H, Klionsky DJ, Ragusa MJ
RgGuinier 7.6 nm
Dmax 34.5 nm
VolumePorod 365 nm3

SASDL69 – Ectodomain of human syndecan-1

UniProt ID: P18827 (23-254) Syndecan-1
Syndecan-1 experimental SAS data
Ectodomain of human syndecan-1 Rg histogram
Sample: Syndecan-1 monomer, 27 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Sep 26
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 5.3 nm

SASDL79 – Ectodomain of human syndecan-2

UniProt ID: P34741 (19-144) Syndecan-2
Syndecan-2 experimental SAS data
Ectodomain of human syndecan-2 Rg histogram
Sample: Syndecan-2 monomer, 17 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 31
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 4.3 nm

SASDL89 – Ectodomain of human syndecan-3

UniProt ID: O75056 (47-387) Syndecan-3
Syndecan-3 experimental SAS data
Ectodomain of human syndecan-3 Rg histogram
Sample: Syndecan-3 monomer, 39 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 May 29
Extended disorder at the cell surface: the conformational landscape of the ectodomains of syndecans Matrix Biology Plus :100081 (2021)
Gondelaud F, Bouakil M, Le Fèvre A, Erica Miele A, Chirot F, Duclos B, Liwo A, Ricard-Blum S
RgGuinier 6.5 nm