SASBDB entries for UniProt ID:

SASDM35 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.0 mg/ml

UniProt ID: P62508 (122-458) Estrogen-related receptor gamma

UniProt ID: None (None-None) Inverse repeat IR3 DNA

Estrogen-related receptor gammaInverse repeat IR3 DNA experimental SAS data
SASREF MX model
Sample: Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein
Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3
Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules Biochemistry 52(39):6844-6855 (2013)
Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D
RgGuinier 4.1 nm
Dmax 12.8 nm

SASDM55 – GTP-binding domain of Candida albicans Ras-like protein 1

UniProt ID: P0CY32 (2-166) GTP-binding domain of Ras-like protein 1

GTP-binding domain of Ras-like protein 1 experimental SAS data
MODELLER model
Sample: GTP-binding domain of Ras-like protein 1 monomer, 19 kDa protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 3 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets. mBio :e0063823 (2023)
Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S
RgGuinier 1.6 nm
Dmax 4.1 nm
VolumePorod 26 nm3

SASDM65 – Candida albicans Ras-like protein 1

UniProt ID: P0CY32 (2-290) Ras-like protein 1

Ras-like protein 1 experimental SAS data
Candida albicans Ras-like protein 1 Rg histogram
Sample: Ras-like protein 1 monomer, 33 kDa Candida albicans protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 3 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets. mBio :e0063823 (2023)
Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S
RgGuinier 3.6 nm
Dmax 15.1 nm
VolumePorod 56 nm3

SASDM75 – Ras-like protein 1 guanine nucleotide exchange factor region of Candida albicans cell division control protein 25

UniProt ID: P43069 (860-1333) Cell division control protein 25

Cell division control protein 25 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer: 20 mM Na-phosphate, 150 mM NaCl, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets. mBio :e0063823 (2023)
Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S
RgGuinier 3.2 nm
Dmax 11.8 nm
VolumePorod 81 nm3

SASDM85 – GTP-binding domain of Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25

UniProt ID: P0CY32 (2-166) GTP-binding domain of Ras-like protein 1

UniProt ID: P43069 (860-1333) Cell division control protein 25

GTP-binding domain of Ras-like protein 1Cell division control protein 25 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: GTP-binding domain of Ras-like protein 1 monomer, 19 kDa protein
Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer: 20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets. mBio :e0063823 (2023)
Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S
RgGuinier 3.2 nm
Dmax 11.8 nm
VolumePorod 99 nm3

SASDM95 – Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25

UniProt ID: P0CY32 (2-290) Ras-like protein 1

UniProt ID: P43069 (860-1333) Cell division control protein 25

Ras-like protein 1Cell division control protein 25 experimental SAS data
Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25 Rg histogram
Sample: Ras-like protein 1 monomer, 33 kDa Candida albicans protein
Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer: 20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21
Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets. mBio :e0063823 (2023)
Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S
RgGuinier 4.0 nm
Dmax 17.7 nm
VolumePorod 149 nm3

SASDMA5 – cone-rod homeobox protein (a peptide of the DNA binding domain of CRX)

UniProt ID: O43186 (39-98) Cone-rod homeobox protein

Cone-rod homeobox protein experimental SAS data
OTHER model
Sample: Cone-rod homeobox protein monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM sodium phosphate,100 mM NaCl, and 5 mM imidazole, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Jun 15
Structural and functional analysis of the human Cone-rod homeobox transcription factor. Proteins (2022)
Clanor PB, Buchholz C, Hayes JE, Friedman MA, White AM, Enke RA, Berndsen CE
RgGuinier 1.2 nm
Dmax 4.0 nm
VolumePorod 10 nm3

SASDMB5 – Leucine-rich repeat protein SHOC-2

UniProt ID: Q9UQ13 (2-582) Leucine-rich repeat protein SHOC-2

Leucine-rich repeat protein SHOC-2 experimental SAS data
BILBOMD model
Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein
Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jun 20
Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J
RgGuinier 3.2 nm
Dmax 11.2 nm
VolumePorod 85 nm3

SASDMC5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C)

UniProt ID: Q9UQ13 (2-582) Leucine-rich repeat protein SHOC-2

UniProt ID: P36873 (1-323) Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunit experimental SAS data
BILBOMD model
Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein
Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20
Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J
RgGuinier 3.3 nm
Dmax 11.7 nm
VolumePorod 132 nm3

SASDMD5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) and GTPase KRas

UniProt ID: Q9UQ13 (2-582) Leucine-rich repeat protein SHOC-2

UniProt ID: P36873 (1-323) Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

UniProt ID: P01116 (2-150) GTPase KRas

Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunitGTPase KRas experimental SAS data
BILBOMD model
Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein
GTPase KRas monomer, 19 kDa Homo sapiens protein
Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20
Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J
RgGuinier 3.3 nm
Dmax 11.7 nm
VolumePorod 156 nm3