SASBDB entries for UniProt ID:

SASDKL2 – NRD-HEPN - mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)

UniProt ID: P52129 (87-357) NRD-HEPN truncated variant of RnlA endoribonuclease
NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data
MULTIFOXS model
Sample: NRD-HEPN truncated variant of RnlA endoribonuclease dimer, 62 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jun 27
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 88 nm3

SASDK37 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl

UniProt ID: P42196 (1-239) Sensory rhodopsin II from Natronbacterium pharaonis

UniProt ID: P42259 (3-534) Sensory rhodopsin II transducer from Natronomonas pharaonis
Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25
Molecular model of a sensor of two-component signaling system Scientific Reports 11(1) (2021)
Ryzhykau Y, Orekhov P, Rulev M, Vlasov A, Melnikov I, Volkov D, Nikolaev M, Zabelskii D, Murugova T, Chupin V, Rogachev A, Gruzinov A, Svergun D, Brennich M, Gushchin I, Soler-Lopez M, Bothe A, Büldt G, Leonard G, Engelhard M, Kuklin A, Gordeliy V
RgGuinier 7.1 nm
Dmax 35.0 nm

SASDK97 – Bromodomain-containing protein 2, BRD2, tandem bromodomains (2 mg/ml)

UniProt ID: P25440 (71-455) Bromodomain-containing protein 2
Bromodomain-containing protein 2 experimental SAS data
Bromodomain-containing protein 2 Kratky plot
Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25
Multivalent nucleosome scaffolding by bromodomain and extraterminal domain tandem bromodomains. J Biol Chem :108289 (2025)
Olp MD, Bursch KL, Wynia-Smith SL, Nuñez R, Goetz CJ, Jackson V, Smith BC
RgGuinier 4.6 nm
Dmax 17.0 nm
VolumePorod 100 nm3

SASDKZ9 – Transcription elongation factor SPT6 - ΔN Spt6

UniProt ID: P23615 (None-None) Transcription elongation factor SPT6 - ΔN Spt6 variant
Transcription elongation factor SPT6 - ΔN Spt6 variant experimental SAS data
Transcription elongation factor SPT6 - ΔN Spt6 Rg histogram
Sample: Transcription elongation factor SPT6 - ΔN Spt6 variant monomer, 132 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM Hepes; 150 NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly. Nucleic Acids Res (2022)
Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R
RgGuinier 4.7 nm
Dmax 14.4 nm
VolumePorod 288 nm3

SASDL93 – Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 4 mg/mL + UFM1 1.0 mg/ml)

UniProt ID: Q9GZZ9 (57-346) Ubiquitin-like modifier-activating enzyme 5

UniProt ID: P61960 (1-83) Ubiquitin fold modifer 1
Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data
SASREF model
Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Ubiquitin fold modifer 1 monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Oct 29
Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M
RgGuinier 3.1 nm
Dmax 13.0 nm

SASDLP3 – Interleukin 11 complex with IL-11Rα and gp130 D2-D3

UniProt ID: Q14626 (23-319) Interleukin-11 receptor subunit alpha

UniProt ID: A8K3F7 (32-199) Interleukin 11

UniProt ID: P40189 (123-324) Interleukin-6 receptor subunit beta
Interleukin-11 receptor subunit alphaInterleukin 11Interleukin-6 receptor subunit beta experimental SAS data
OTHER model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Interleukin 11 monomer, 18 kDa Homo sapiens protein
Interleukin-6 receptor subunit beta monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 3.6 nm
Dmax 12.9 nm
VolumePorod 127 nm3

SASDL34 – Human Albumin (HNA1)

UniProt ID: P02768 (None-None) Albumin
Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1
Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M
RgGuinier 2.9 nm
Dmax 8.9 nm

SASDLP4 – Superoxide dismutase, SodA

UniProt ID: P00448 (1-206) Superoxide dismutase [Mn]
Superoxide dismutase [Mn] experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Superoxide dismutase [Mn], 23 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1
Protein quaternary structures in solution are a mixture of multiple forms Chemical Science 13(39):11680-11695 (2022)
Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G
RgGuinier 2.3 nm
Dmax 7.3 nm
VolumePorod 54 nm3

SASDLZ4 – Bacillus subtilis phosphoglucosmine mutase GlmM

UniProt ID: O34824 (1-448) Phosphoglucosamine mutase
Phosphoglucosamine mutase experimental SAS data
Phosphoglucosamine mutase Kratky plot
Sample: Phosphoglucosamine mutase dimer, 101 kDa Bacillus subtilis (strain … protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 21
Structural basis for the inhibition of the Bacillus subtilis c-di-AMP cyclase CdaA by the phosphoglucomutase GlmM Journal of Biological Chemistry :101317 (2021)
Pathania M, Tosi T, Millership C, Hoshiga F, Morgan R, Freemont P, Gründling A
RgGuinier 3.7 nm
Dmax 12.2 nm
VolumePorod 140 nm3

SASDL46 – 14-3-3zeta AXH-C complex

UniProt ID: P63104 (None-None) 14-3-3 protein zeta/delta

UniProt ID: P54253 (None-None) Ataxin-1 AXH-C
14-3-3 protein zeta/deltaAtaxin-1 AXH-C experimental SAS data
MULTIFOXS model
Sample: 14-3-3 protein zeta/delta dimer, 53 kDa Homo sapiens protein
Ataxin-1 AXH-C dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 13
A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1. J Mol Biol :167174 (2021)
Leysen S, Jane Burnley R, Rodriguez E, Milroy LG, Soini L, Adamski CJ, Nitschke L, Davis R, Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J
RgGuinier 4.8 nm
Dmax 19.8 nm