Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDBU8 – Probable ATP-dependent RNA helicase DDX58 (Delta-CARDs) plus 8mer hairpin dsRNA/AMP-PNP (SEC-peak2)

UniProt ID: (1-20) 5´ppp 8mer hairpin dsRNA

UniProt ID: O95786 (239-925) Probable ATP-dependent RNA helicase DDX58 (without CARDs)

5´ppp 8mer hairpin dsRNAProbable ATP-dependent RNA helicase DDX58 (without CARDs) experimental SAS data

5´ppp 8mer hairpin dsRNA Probable ATP-dependent RNA helicase DDX58 (without CARDs) Kratky plot

Sample:	5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA Probable ATP-dependent RNA helicase DDX58 (without CARDs) dimer, 160 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 27

Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ

R_g^Guinier	3.8	nm
D_max	15.4	nm
Volume^Porod	226	nm³

SASDBV8 – Probable ATP-dependent RNA helicase DDX58 (Delta-CARDs) plus 8mer hairpin dsRNA/ADP-AlFx (SEC-peak2)

UniProt ID: (1-20) 5´ppp 8mer hairpin dsRNA

UniProt ID: O95786 (239-925) Probable ATP-dependent RNA helicase DDX58 (without CARDs)

Sample:	5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA Probable ATP-dependent RNA helicase DDX58 (without CARDs) dimer, 160 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20

Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ

R_g^Guinier	3.9	nm
D_max	16.0	nm
Volume^Porod	230	nm³

SASDB59 – Recombinant monomeric human Properdin

UniProt ID: P27918 (None-None) Human recombinant Properdin TSR 0-3

UniProt ID: P27918 (None-None) Human recombinant Properdin TSR 4-6

Human recombinant Properdin TSR 0-3Human recombinant Properdin TSR 4-6 experimental SAS data

Sample:	Human recombinant Properdin TSR 0-3 monomer, 25 kDa Homo sapiens protein Human recombinant Properdin TSR 4-6 monomer, 24 kDa Homo sapiens protein
Buffer:	10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Aug 16

Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR

R_g^Guinier	5.1	nm
D_max	18.0	nm

SASDB69 – E244K monomeric human Properdin

UniProt ID: P27918 (None-None) E244K Human Properdin

E244K Human Properdin experimental SAS data

Sample:	E244K Human Properdin monomer, 49 kDa Homo sapiens protein
Buffer:	10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jul 27

R_g^Guinier	4.1	nm
D_max	15.0	nm

SASDB79 – Basic domain of human telomeric repeat-binding factor 2 (TRF2) in complex with telomeric DNA duplex

UniProt ID: Q15554 (42-86) Basic domain of telomeric repeat-binding factor 2

UniProt ID: (None-None) telomere DNA duplex

Basic domain of telomeric repeat-binding factor 2telomere DNA duplex experimental SAS data

Sample:	Basic domain of telomeric repeat-binding factor 2 monomer, 5 kDa Homo sapiens protein Telomere DNA duplex monomer, 11 kDa DNA
Buffer:	20 mM Tris-HCl, 50 mM LiCl, pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 May 3

Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
Necasová I, Janoušková E, Klumpler T, Hofr C

R_g^Guinier	1.7	nm
D_max	6.0	nm
Volume^Porod	20	nm³

SASDB99 – Extracellular assembly of the human TSLP:TSLPR:IL-7Ralpha complex

UniProt ID: Q969D9 (None-None) Thymic stromal lymphopoietin

UniProt ID: P16871 (21-239) Interleukin-7 receptor subunit alpha

UniProt ID: Q9HC73 (25-221) Cytokine receptor-like factor 2

Thymic stromal lymphopoietinInterleukin-7 receptor subunit alphaCytokine receptor-like factor 2 experimental SAS data

Sample:	Thymic stromal lymphopoietin monomer, 15 kDa Homo sapiens protein Interleukin-7 receptor subunit alpha monomer, 26 kDa Homo sapiens protein Cytokine receptor-like factor 2 monomer, 24 kDa Homo sapiens protein
Buffer:	10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Apr 8

Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun 8:14937 (2017)
Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN

R_g^Guinier	3.1	nm
D_max	10.3	nm
Volume^Porod	98	nm³

SASDBA9 – E. coli CcdB2-CcdA2-CcdB2 toxin/antitoxin heterohexamer complex

UniProt ID: P62554 (1-101) Toxin CcdB

UniProt ID: P62552 (1-72) Antitoxin CcdA

UniProt ID: P62554 (None-None) Toxin CcdB

Toxin CcdBAntitoxin CcdAToxin CcdB experimental SAS data

Sample:	Toxin CcdB dimer, 23 kDa Escherichia coli protein Antitoxin CcdA dimer, 17 kDa Escherichia coli protein Toxin CcdB dimer, 23 kDa Escherichia coli protein
Buffer:	10 mM Tris 50 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jul 24

Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. Nucleic Acids Res 45(6):2937-2950 (2017)
Vandervelde A, Drobnak I, Hadži S, Sterckx YG, Welte T, De Greve H, Charlier D, Efremov R, Loris R, Lah J

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	92	nm³

SASDBB9 – GDP bound form of full length ObgE from E.coli (ObgE_FL with GDP)

UniProt ID: P42641 (1-390) GTPase ObgE/CgtA

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W

R_g^Guinier	3.5	nm
D_max	16.0	nm
Volume^Porod	94673	nm³

SASDBC9 – GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP)

UniProt ID: P42641 (1-340) GTPase ObgE/CgtA

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, on 2015 Feb 1

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	70673	nm³

SASDBD9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

UniProt ID: Q316W1 (None-None) ABC transporter periplasmic substrate-binding protein

ABC transporter periplasmic substrate-binding protein experimental SAS data

Sample:	ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer:	5 mM Tris-HCl, pH: 7.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7

Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
Otrelo-Cardoso AR, Nair RR, Correia MAS, Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG

R_g^Guinier	2.4	nm
D_max	9.6	nm
Volume^Porod	51	nm³

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