SASBDB entries for UniProt ID:

SASDB59 – Recombinant monomeric human Properdin

UniProt ID: P27918 (None-None) Human recombinant Properdin TSR 0-3

UniProt ID: P27918 (None-None) Human recombinant Properdin TSR 4-6
Human recombinant Properdin TSR 0-3Human recombinant Properdin TSR 4-6 experimental SAS data
CORAL model
Sample: Human recombinant Properdin TSR 0-3 monomer, 25 kDa Homo sapiens protein
Human recombinant Properdin TSR 4-6 monomer, 24 kDa Homo sapiens protein
Buffer: 10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 16
Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR
RgGuinier 5.1 nm
Dmax 18.0 nm

SASDB69 – E244K monomeric human Properdin

UniProt ID: P27918 (None-None) E244K Human Properdin
E244K Human Properdin experimental SAS data
CORAL model
Sample: E244K Human Properdin monomer, 49 kDa Homo sapiens protein
Buffer: 10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 27
Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR
RgGuinier 4.1 nm
Dmax 15.0 nm

SASDB79 – Basic domain of human telomeric repeat-binding factor 2 (TRF2) in complex with telomeric DNA duplex

UniProt ID: Q15554 (42-86) Basic domain of telomeric repeat-binding factor 2

UniProt ID: (None-None) telomere DNA duplex
Basic domain of telomeric repeat-binding factor 2telomere DNA duplex experimental SAS data
MONSA model
Sample: Basic domain of telomeric repeat-binding factor 2 monomer, 5 kDa Homo sapiens protein
Telomere DNA duplex monomer, 11 kDa DNA
Buffer: 20 mM Tris-HCl, 50 mM LiCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 May 3
Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
Necasová I, Janoušková E, Klumpler T, Hofr C
RgGuinier 1.7 nm
Dmax 6.0 nm
VolumePorod 20 nm3

SASDB99 – Extracellular assembly of the human TSLP:TSLPR:IL-7Ralpha complex

UniProt ID: Q969D9 (None-None) Thymic stromal lymphopoietin

UniProt ID: P16871 (21-239) Interleukin-7 receptor subunit alpha

UniProt ID: Q9HC73 (25-221) Cytokine receptor-like factor 2
Thymic stromal lymphopoietinInterleukin-7 receptor subunit alphaCytokine receptor-like factor 2 experimental SAS data
ALLOSMOD model
Sample: Thymic stromal lymphopoietin monomer, 15 kDa Homo sapiens protein
Interleukin-7 receptor subunit alpha monomer, 26 kDa Homo sapiens protein
Cytokine receptor-like factor 2 monomer, 24 kDa Homo sapiens protein
Buffer: 10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 8
Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun 8:14937 (2017)
Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN
RgGuinier 3.1 nm
Dmax 10.3 nm
VolumePorod 98 nm3

SASDBA9 – E. coli CcdB2-CcdA2-CcdB2 toxin/antitoxin heterohexamer complex

UniProt ID: P62554 (1-101) Toxin CcdB

UniProt ID: P62552 (1-72) Antitoxin CcdA

UniProt ID: P62554 (None-None) Toxin CcdB
Toxin CcdBAntitoxin CcdAToxin CcdB experimental SAS data
MODELLER model
Sample: Toxin CcdB dimer, 23 kDa Escherichia coli protein
Antitoxin CcdA dimer, 17 kDa Escherichia coli protein
Toxin CcdB dimer, 23 kDa Escherichia coli protein
Buffer: 10 mM Tris 50 mM NaCl, pH: 7.3
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Jul 24
Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. Nucleic Acids Res 45(6):2937-2950 (2017)
Vandervelde A, Drobnak I, Hadži S, Sterckx YG, Welte T, De Greve H, Charlier D, Efremov R, Loris R, Lah J
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 92 nm3

SASDBB9 – GDP bound form of full length ObgE from E.coli (ObgE_FL with GDP)

UniProt ID: P42641 (1-390) GTPase ObgE/CgtA
GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.5 nm
Dmax 16.0 nm
VolumePorod 94673 nm3

SASDBC9 – GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP)

UniProt ID: P42641 (1-340) GTPase ObgE/CgtA
GTPase ObgE/CgtA experimental SAS data
DAMMIN model
Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, on 2015 Feb 1
Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 70673 nm3

SASDBD9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

UniProt ID: Q316W1 (None-None) ABC transporter periplasmic substrate-binding protein
ABC transporter periplasmic substrate-binding protein experimental SAS data
SREFLEX model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
Otrelo-Cardoso AR, Nair RR, Correia MAS, Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 51 nm3

SASDBE9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

UniProt ID: Q316W1 (None-None) ABC transporter periplasmic substrate-binding protein
ABC transporter periplasmic substrate-binding protein experimental SAS data
SREFLEX model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
Otrelo-Cardoso AR, Nair RR, Correia MAS, Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 49 nm3

SASDBF9 – ABC transporter periplasmic substrate-binding protein TupA measured in the presence of tungstate

UniProt ID: Q316W1 (None-None) ABC transporter periplasmic substrate-binding protein
ABC transporter periplasmic substrate-binding protein experimental SAS data
BUNCH model
Sample: ABC transporter periplasmic substrate-binding protein monomer, 30 kDa Desulfovibrio alaskensis protein
Buffer: 5 mM Tris-HCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 7
Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20. Sci Rep 7(1):5798 (2017)
Otrelo-Cardoso AR, Nair RR, Correia MAS, Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 46 nm3