Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

UniProt ID: P24300 (1-388) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE

R_g^Guinier	3.3	nm
D_max	9.2	nm
Volume^Porod	229	nm³

SASDCL2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 3

UniProt ID: Q9X721 (883-1118) Class1 collagenase

Class1 collagenase experimental SAS data

Sample:	Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
Buffer:	10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14

Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R

R_g^Guinier	2.4	nm
D_max	7.5	nm
Volume^Porod	36	nm³

SASDCM2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 4

UniProt ID: Q9X721 (883-1118) Class1 collagenase collagen-binding domain

Class1 collagenase collagen-binding domain experimental SAS data

Sample:	Class1 collagenase collagen-binding domain monomer, 27 kDa Hathewaya histolytica protein
Buffer:	10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14

Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R

R_g^Guinier	2.4	nm
D_max	9.8	nm
Volume^Porod	33	nm³

SASDCN2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 5

UniProt ID: Q9X721 (883-1118) Class1 collagenase

Sample:	Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
Buffer:	10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Sep 14

Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R

R_g^Guinier	2.5	nm
D_max	9.4	nm
Volume^Porod	36	nm³

SASDCP2 – Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 6

UniProt ID: Q9X721 (883-1118) Class1 collagenase

Sample:	Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
Buffer:	10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14

Structural characterization of bacterial collagenases University of Arkansas PhD thesis 2431 (2017)
Bauer R

R_g^Guinier	2.6	nm
D_max	12.8	nm
Volume^Porod	33	nm³

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

UniProt ID: P0DP33 (2-149) Calmodulin-1

4Ca2+-calmodulin - Xenopus laevis Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	25	nm³

SASDCZ2 – Yeast CTDsome: transcription termination factor Rtt103p/C-terminal domain of DNA-directed RNA polymerase II subunit RPB1 (Rtt103 1-246-phospho-mimetic complex)

UniProt ID: Q05543 (1-246) Regulator of Ty1 transposition protein 103

UniProt ID: P04050 (None-None) DNA-directed RNA polymerase II subunit RPB1

Regulator of Ty1 transposition protein 103DNA-directed RNA polymerase II subunit RPB1 experimental SAS data

Sample:	Regulator of Ty1 transposition protein 103 dimer, 57 kDa Saccharomyces cerevisiae protein DNA-directed RNA polymerase II subunit RPB1 monomer, 13 kDa Saccharomyces cerevisiae protein
Buffer:	25 mM KH2PO4, 300 mM KCl, 10 mM BME, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 Apr 12

Structure and dynamics of the RNAPII CTDsome with Rtt103. Proc Natl Acad Sci U S A 114(42):11133-11138 (2017)
Jasnovidova O, Klumpler T, Kubicek K, Kalynych S, Plevka P, Stefl R

R_g^Guinier	5.3	nm
D_max	21.0	nm
Volume^Porod	561	nm³

SASDC23 – Colicin N WT

UniProt ID: P08083 (1-387) Colicin N

Sample:	Colicin N monomer, 43 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	74	nm³

SASDC33 – Colicin N delta 1-39

UniProt ID: P08083 (40-387) Colicin N delta 1-39

Colicin N delta 1-39 experimental SAS data

Sample:	Colicin N delta 1-39 monomer, 39 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	2.7	nm
D_max	9.0	nm
Volume^Porod	60	nm³

SASDC43 – Colicin K145A mutant

UniProt ID: P08083 (1-387) Colicin N K145A mutant

Colicin N K145A mutant experimental SAS data

Sample:	Colicin N K145A mutant monomer, 43 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	3.2	nm
D_max	13.6	nm
Volume^Porod	68	nm³

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