SASBDB entries for UniProt ID:

SASDPN2 – apo-Pyrococcus furiosus argonaute protein at 95 °C

UniProt ID: Q8U3D2 (5-770) Piwi domain-containing protein
Piwi domain-containing protein experimental SAS data
GASBOR model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.2 nm
Dmax 9.7 nm
VolumePorod 187 nm3

SASDPR3 – RTX719-K983R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA)

UniProt ID: None (719-1706) Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294
Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data
RTX719-K983R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram
Sample: Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer: 50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 17
Acyl chains stabilize the acylated domain and determine the receptor-mediated interaction of the Bordetella adenylate cyclase toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L
RgGuinier 5.3 nm
Dmax 26.3 nm
VolumePorod 187 nm3

SASDPF4 – P2X7 ballast domain in complex with Ca2+-calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1

UniProt ID: Q99572 (395-595) P2X purinoceptor 7
Calmodulin-1P2X purinoceptor 7 experimental SAS data
Calmodulin-1 P2X purinoceptor 7 Kratky plot
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
P2X purinoceptor 7 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 73 nm3

SASDPR5 – L-methionine gamma-lyase from Clostridium sporogenes

UniProt ID: A0A1L3NFS6 (1-400) L-methionine gamma-lyase (K272S)
L-methionine gamma-lyase (K272S) experimental SAS data
CHIMERA model
Sample: L-methionine gamma-lyase (K272S) tetramer, 174 kDa Clostridium sporogenes protein
Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19
Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR Biochemical and Biophysical Research Communications :149319 (2023)
Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I
RgGuinier 3.7 nm
Dmax 14.5 nm
VolumePorod 211 nm3

SASDQF4 – ESX-1 secretion-associated protein EspB

UniProt ID: P9WJD9 (2-348) ESX-1 secretion-associated protein EspB
ESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspB heptamer, 261 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 5.9 nm
Dmax 18.8 nm
VolumePorod 911 nm3

SASDQ79 – Mutant 7-deazapurine in DNA protein A (D95A) bound to a 28 base pair DNA substrate (2-state model: complex plus free DNA)

UniProt ID: None (None-None) Custom 28 base pair double stranded DNA

UniProt ID: P0DW33 (2-416) DNA-guanine transglycosylase - D95A mutant
Custom 28 base pair double stranded DNADNA-guanine transglycosylase - D95A mutant experimental SAS data
GROMACS model
Sample: Custom 28 base pair double stranded DNA monomer, 17 kDa Synthetic, purchased from … DNA
DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 104 nm3

SASDRZ4 – K214/R260 mutant of Rabies virus CVS Phosphoprotein Isoform 3 (KRm CVS P3)

UniProt ID: P22363 (53-297) Phosphoprotein (Isoform P3; K214A, R260A, D289N, C297S)
Phosphoprotein (Isoform P3; K214A, R260A, D289N, C297S) experimental SAS data
EOM/RANCH model
Sample: Phosphoprotein (Isoform P3; K214A, R260A, D289N, C297S) dimer, 55 kDa Rabies virus (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Mar 7
Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein. Nat Commun 16(1):9491 (2025)
Rawlinson SM, Chakraborty S, Sethi A, David CT, Harrison AR, Bird LE, Rozario AM, Uthishtran S, Ardipradja K, Zhao T, Oksayan S, Jans DA, Ang CS, Lu ZH, Yan F, Williamson NA, Arumugam S, Sundaramoorthy V, Bell TDM, Gooley PR, Moseley GW
RgGuinier 4.6 nm
Dmax 22.5 nm
VolumePorod 180 nm3

SASDRG5 – A ssr1698 Dri1 hemoprotein, wild-type (apo) variant + heme

UniProt ID: P73129 (1-96) Ssr1698 protein
Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 29 nm3

SASDRQ5 – Small beta-barrel domain in the N-terminal region of the Translocated Intimin Receptor (Tir76-180)

UniProt ID: B7UM99 (76-180) Translocated intimin receptor Tir
Translocated intimin receptor Tir experimental SAS data
Translocated intimin receptor Tir Kratky plot
Sample: Translocated intimin receptor Tir monomer, 12 kDa Escherichia coli O127:H6 … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Nov 21
The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection. Commun Biol 7(1):179 (2024)
Vieira MFM, Hernandez G, Zhong Q, Arbesú M, Veloso T, Gomes T, Martins ML, Monteiro H, Frazão C, Frankel G, Zanzoni A, Cordeiro TN
RgGuinier 1.7 nm
Dmax 7.0 nm
VolumePorod 21 nm3

SASDRL6 – SH2-SH3-SH2 domains of Ras GTPase-activating protein 1 (p120RasGAP) bound to a doubly phosphorylated p190RhoGAP peptide

UniProt ID: Q9NRY4 (1083-1111) Rho GTPase-activating protein 35

UniProt ID: P20936 (174-444) Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S)
Rho GTPase-activating protein 35Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) experimental SAS data
Rho GTPase-activating protein 35 Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) Kratky plot
Sample: Rho GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein
Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 350 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 2.4 nm
Dmax 7.7 nm
VolumePorod 44 nm3