SASBDB entries for UniProt ID:

SASDCL9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA) in complex with calmodulin

UniProt ID: P0DKX7 (1-364) Bifunctional hemolysin/adenylate cyclase

UniProt ID: P62158 (None-None) Calmodulin
Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data
BUNCH model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 78 nm3

SASDCM9 – Ammonium transporter Ks-Amt5

UniProt ID: Q1Q357 (None-None) ammonium sensor/transducer
ammonium sensor/transducer experimental SAS data
CORAL model
Sample: Ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 1
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 511 nm3

SASDCN9 – Ks-Amt5-HK (apo)

UniProt ID: Q1Q357 (None-None) Ammonium transporter histidine kinase domain
Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: Ammonium transporter histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 71 nm3

SASDCP9 – Ks-Amt5-HK (+10uM APPCP)

UniProt ID: Q1Q357 (None-None) Ammonium transporter histidine kinase domain
Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: Ammonium transporter histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 53 nm3

SASDCQ9 – Ks-Amt5-HK (+10uM ATP)

UniProt ID: Q1Q357 (None-None) Ammonium transporter histidine kinase domain
Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: Ammonium transporter histidine kinase domain monomer, 30 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.2 nm
Dmax 7.7 nm
VolumePorod 53 nm3

SASDCR9 – Kif2A-tubulin-DARP complex in the presence of AMP-PNP

UniProt ID: None (None-None) Designed Ankyrin Repeat Protein D1

UniProt ID: O00139 (153-553) Kinesin-like protein KIF2A

UniProt ID: P81947 (None-None) Tubulin alpha-1B chain

UniProt ID: Q6B856 (None-None) Tubulin beta-2B chain
Designed Ankyrin Repeat Protein D1Kinesin-like protein KIF2ATubulin alpha-1B chainTubulin beta-2B chain experimental SAS data
DAMMIF model
Sample: Designed Ankyrin Repeat Protein D1 monomer, 18 kDa synthetic construct protein
Kinesin-like protein KIF2A monomer, 48 kDa Homo sapiens protein
Tubulin alpha-1B chain dimer, 100 kDa Bos taurus protein
Tubulin beta-2B chain dimer, 100 kDa Bos taurus protein
Buffer: HEPES 20 mM, MgCl2 1mM, NaCl 150mM, pH: 7.2
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 16
Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate. Nat Commun 9(1):2628 (2018)
Trofimova D, Paydar M, Zara A, Talje L, Kwok BH, Allingham JS
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 374 nm3

SASDCS9 – Ammonium transporter Ks-Amt5 (Batch)

UniProt ID: Q1Q357 (None-None) ammonium sensor/transducer
ammonium sensor/transducer experimental SAS data
ammonium sensor/transducer Kratky plot
Sample: Ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 482 nm3

SASDCY9 – Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii

UniProt ID: A6Q0K5 (28-107) Calvin cycle protein CP12, chloroplastic
Calvin cycle protein CP12, chloroplastic experimental SAS data
Oxidised chloroplastic calvin cycle protein CP12 from C. reinhardtii Rg histogram
Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein
Buffer: 50 mM phosphate buffer, 50 mM NaCl, 20 mM oxidized DTT, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 3
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 22 nm3

SASDCZ9 – Glyceraldehyde-3-phosphate dehydrogenase from C. reinhardtii

UniProt ID: A8HP84 (None-None) Glyceraldehyde-3-phosphate dehydrogenase
Glyceraldehyde-3-phosphate dehydrogenase experimental SAS data
MODELLER model
Sample: Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein
Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 7
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 206 nm3

SASDD22 – Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii)

UniProt ID: A6Q0K5 (28-107) Calvin cycle protein CP12, chloroplastic

UniProt ID: A8HP84 (None-None) Glyceraldehyde-3-phosphate dehydrogenase
Calvin cycle protein CP12, chloroplasticGlyceraldehyde-3-phosphate dehydrogenase experimental SAS data
Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 (C. reinhardtii) Rg histogram
Sample: Calvin cycle protein CP12, chloroplastic monomer, 11 kDa Chlamydomonas reinhardtii protein
Glyceraldehyde-3-phosphate dehydrogenase tetramer, 148 kDa Chlamydomonas reinhardtii protein
Buffer: 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2010 Dec 8
Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase. J Mol Biol 430(8):1218-1234 (2018)
Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V
RgGuinier 3.8 nm
Dmax 19.0 nm
VolumePorod 251 nm3