SASBDB entries for UniProt ID:

SASDDA5 – Neurexin 1a L5L6 with ss6 insert

UniProt ID: Q9ULB1 (904-1307) Neurexin 1a L5L6 with ss6 insert
Neurexin 1a L5L6 with ss6 insert experimental SAS data
Neurexin 1a L5L6 with ss6 insert Rg histogram
Sample: Neurexin 1a L5L6 with ss6 insert monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2016 Sep 26
Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer. J Mol Biol 430(21):4325-4343 (2018)
Liu J, Misra A, Reddy MVVVS, White MA, Ren G, Rudenko G
RgGuinier 3.2 nm
Dmax 12.4 nm
VolumePorod 70 nm3

SASDDB5 – MsbA in stealth nanodisc (SANS, 100% D2O)

UniProt ID: P60752 (None-None) Lipid A export ATP-binding/permease protein MsbA

UniProt ID: None (None-None) Membrane scaffold protein 1D1 (deuterated, 75%)

UniProt ID: None (None-None) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)
Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
SASREF CV model
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa Escherichia coli protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SANS data collected at D11, ILL on 2017 Mar 9
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 189 nm3

SASDDC5 – MsbA in stealth nanodisc (SANS, 100% D2O) + 1 mM ADP

UniProt ID: P60752 (None-None) Lipid A export ATP-binding/permease protein MsbA

UniProt ID: None (None-None) Membrane scaffold protein 1D1 (deuterated, 75%)

UniProt ID: None (None-None) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)
Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa Escherichia coli protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli
Buffer: 30 mM Tris, 150 mM NaCl, 1 mM ADP, pH: 7.5
Experiment: SANS data collected at D11, ILL on 2017 Mar 9
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 173 nm3

SASDDD5 – MsbA in stealth nanodisc (SAXS)

UniProt ID: P60752 (None-None) Lipid A export ATP-binding/permease protein MsbA

UniProt ID: None (None-None) Membrane scaffold protein 1D1 (deuterated, 75%)

UniProt ID: None (None-None) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)
Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa Escherichia coli protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 607 nm3

SASDDE5 – NBD-MsbA (apo)

UniProt ID: P60752 (None-None) Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA monomer, 27 kDa Escherichia coli protein
Buffer: 30 mM Tris, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 30
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 47 nm3

SASDDF5 – NBD-MsbA (+1mM ADP)

UniProt ID: P60752 (None-None) Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA monomer, 27 kDa Escherichia coli protein
Buffer: 30 mM Tris, 150 mM NaCl, 0.5 mM TCEP, 1 mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 30
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.1 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDDT5 – Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)

UniProt ID: Q9DH28 (None-None) Nonstructural protein sigma NS

UniProt ID: None (None-None) 20mer RNA (unstructured)
Nonstructural protein sigma NS20mer RNA (unstructured) experimental SAS data
Nonstructural protein sigma NS 20mer RNA (unstructured) Kratky plot
Sample: Nonstructural protein sigma NS octamer, 325 kDa Avian orthoreovirus protein
20mer RNA (unstructured) dimer, 13 kDa RNA
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 25
Stability of local secondary structure determines selectivity of viral RNA chaperones. Nucleic Acids Res (2018)
Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R
RgGuinier 7.8 nm
Dmax 38.0 nm
VolumePorod 964 nm3

SASDDU5 – Nonstructural protein sigma NS - apoprotein

UniProt ID: Q9DH28 (None-None) Nonstructural protein sigma NS
Nonstructural protein sigma NS experimental SAS data
Nonstructural protein sigma NS Kratky plot
Sample: Nonstructural protein sigma NS hexamer, 244 kDa Avian orthoreovirus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 25
Stability of local secondary structure determines selectivity of viral RNA chaperones. Nucleic Acids Res (2018)
Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R
RgGuinier 5.5 nm
Dmax 23.1 nm
VolumePorod 670 nm3

SASDDV5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum

UniProt ID: A5I6N2 (2-292) 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 159 nm3

SASDDW5 – 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS-apo) from C. botulinum + pyruvate

UniProt ID: A5I6N2 (2-292) 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum experimental SAS data
4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum Kratky plot
Sample: 4-hydroxy-tetrahydrodipicolinate synthase from Clostridium botulinum tetramer, 126 kDa Clostridium botulinum protein
Buffer: 20mM Tris, 150mM NaCl, 5mM sodium pyruvate, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2010 Nov 26
Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target. Structure 26(7):948-959.e5 (2018)
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA
RgGuinier 3.3 nm
Dmax 8.9 nm
VolumePorod 165 nm3