SASBDB entries for UniProt ID:

SASDDX4 – Wild-type Protein sex-lethal

UniProt ID: P19339 (123-294) Protein sex-lethal
Protein sex-lethal experimental SAS data
Protein sex-lethal Kratky plot
Sample: Protein sex-lethal monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 26 nm3

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

UniProt ID: P19339 (123-294) Protein sex-lethal mutant
Protein sex-lethal mutant experimental SAS data
Protein sex-lethal mutant with 10GS-linker Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 2
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 27 nm3

SASDDZ4 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU

UniProt ID: P19339 (123-294) Protein sex-lethal mutant

UniProt ID: None (None-None) RNA decaneucleotide U8GU
Protein sex-lethal mutantRNA decaneucleotide U8GU experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
RNA decaneucleotide U8GU monomer, 3 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 3
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.2 nm
Dmax 7.8 nm
VolumePorod 34 nm3

SASDD25 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8

UniProt ID: P19339 (123-294) Protein sex-lethal mutant

UniProt ID: None (None-None) RNA decaneucleotide UGU8
Protein sex-lethal mutantRNA decaneucleotide UGU8 experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8 Rg histogram
Sample: Protein sex-lethal mutant dimer, 41 kDa Drosophila melanogaster protein
RNA decaneucleotide UGU8 dimer, 6 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 12
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 41 nm3

SASDD45 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein

UniProt ID: Q63NA5 (1-134) Type II toxin-antitoxin system HicB family antitoxin
Type II toxin-antitoxin system HicB family antitoxin experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 28
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 112 nm3

SASDD55 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein - bound to addiction module toxin, HicA

UniProt ID: Q63NA5 (1-134) Type II toxin-antitoxin system HicB family antitoxin

UniProt ID: Q63NA6 (None-None) Addiction module toxin, HicA
Type II toxin-antitoxin system HicB family antitoxinAddiction module toxin, HicA experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Addiction module toxin, HicA monomer, 7 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 27
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 110 nm3

SASDD65 – Ubiquitinating/deubiquitinating enzyme SdeA 207 914

UniProt ID: Q5ZTK4 (277-907) Ubiquitinating/deubiquitinating enzyme SdeA
Ubiquitinating/deubiquitinating enzyme SdeA experimental SAS data
OTHER model
Sample: Ubiquitinating/deubiquitinating enzyme SdeA monomer, 72 kDa Legionella pneumophila subsp. … protein
Buffer: 10 mM HEPES 150 mM NaCl 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26
Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination. Nature 557(7707):734-738 (2018)
Kalayil S, Bhogaraju S, Bonn F, Shin D, Liu Y, Gan N, Basquin J, Grumati P, Luo ZQ, Dikic I
RgGuinier 3.5 nm
Dmax 11.2 nm
VolumePorod 128 nm3

SASDD75 – 'TG'-interacting factor homeodomain in solution (Homeobox protein TGIF1)

UniProt ID: Q15583 (151-248) Homeobox protein TGIF1
Homeobox protein TGIF1 experimental SAS data
'TG'-interacting factor homeodomain in solution (Homeobox protein TGIF1) Rg histogram
Sample: Homeobox protein TGIF1 dimer, 23 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 18
'TG' interacting factor
Ewelina Guca
RgGuinier 2.7 nm
Dmax 9.2 nm
VolumePorod 42 nm3

SASDD85 – PDZK1 Domain 1-4

UniProt ID: Q5T2W7 (7-460) Uncharacterized protein C1orf159
Uncharacterized protein C1orf159 experimental SAS data
GASBOR model
Sample: Uncharacterized protein C1orf159 monomer, 53 kDa Homo sapiens protein
Buffer: 20mM Hepes 150 NaCl 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 28
Probing the Architecture of a Multi-PDZ Domain Protein: Structure of PDZK1 in Solution. Structure 26(11):1522-1533.e5 (2018)
Hajizadeh NR, Pieprzyk J, Skopintsev P, Flayhan A, Svergun DI, Löw C
RgGuinier 3.9 nm
Dmax 15.0 nm
VolumePorod 120 nm3

SASDD95 – Neurexin 1a L5L6

UniProt ID: Q9ULB1 (904-1307) Neurexin 1a L5L6
Neurexin 1a L5L6 experimental SAS data
Neurexin 1a L5L6 Rg histogram
Sample: Neurexin 1a L5L6 monomer, 44 kDa protein
Buffer: 20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, Sealy Center For Structural Biology, UTMB-G on 2016 Sep 6
Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer. J Mol Biol 430(21):4325-4343 (2018)
Liu J, Misra A, Reddy MVVVS, White MA, Ren G, Rudenko G
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 69 nm3