SASBDB entries for UniProt ID:

SASDEM9 – anEcTFE: Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme complex

UniProt ID: P77399 (1-714) Fatty acid oxidation complex subunit alpha

UniProt ID: P77399 (None-None) anaerobic Fatty acid oxidation complex subunit alpha

UniProt ID: P77399 (None-None) anaerobic Fatty acid oxidation complex subunit alpha

UniProt ID: P77399 (None-None) anaerobic Fatty acid oxidation complex subunit alpha

UniProt ID: P76503 (None-None) anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit

UniProt ID: P76503 (None-None) anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit
Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic 3-ketoacyl-CoA thiolase FadI beta subunitanaerobic 3-ketoacyl-CoA thiolase FadI beta subunit experimental SAS data
DAMMIN model
Sample: Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain … protein
Anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli protein
Anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli protein
Anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli protein
Anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit dimer, 96 kDa Escherichia coli protein
Anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit dimer, 96 kDa Escherichia coli protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, 0.05% C12E9 (1-O-(n-Dodecyl)-nonaethyleneglycol), 2.5 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2015 Sep 22
Complementary substrate specificity and distinct quaternary assembly of the Escherichia coli aerobic and anaerobic beta-oxidation trifunctional enzyme complexes. Biochem J (2019)
Sah-Teli SK, Hynönen MJ, Schmitz W, Geraets JA, Seitsonen J, Pedersen JS, Butcher SJ, Wierenga RK, Venkatesan R
RgGuinier 6.2 nm
Dmax 19.6 nm
VolumePorod 856 nm3

SASDEN9 – HcpR transcriptional regulator of Porphyromonas gingivalis

UniProt ID: Q7MVK4 (1-227) Hcp Transcriptional regulator
Hcp Transcriptional regulator experimental SAS data
HcpR transcriptional regulator of Porphyromonas gingivalis Rg histogram
Sample: Hcp Transcriptional regulator dimer, 51 kDa Porphyromonas gingivalis protein
Buffer: 25 mM Tris 150 mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Jan 24
Nitrosative stress sensing in Porphyromonas gingivalis: structure of and heme binding by the transcriptional regulator HcpR. Acta Crystallogr D Struct Biol 75(Pt 4):437-450 (2019)
Belvin BR, Musayev FN, Burgner J, Scarsdale JN, Escalante CR, Lewis JP
RgGuinier 2.9 nm
Dmax 10.5 nm
VolumePorod 76 nm3

SASDEP9 – Cyclic GMP-AMP synthase (cGAS)

UniProt ID: Q8N884 (1-522) Cyclic GMP-AMP synthase
Cyclic GMP-AMP synthase experimental SAS data
Cyclic GMP-AMP synthase (cGAS) Rg histogram
Sample: Cyclic GMP-AMP synthase monomer, 61 kDa Homo sapiens protein
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 25
cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity. EMBO Rep (2019)
Liu H, Moura-Alves P, Pei G, Mollenkopf HJ, Hurwitz R, Wu X, Wang F, Liu S, Ma M, Fei Y, Zhu C, Koehler AB, Oberbeck-Mueller D, Hahnke K, Klemm M, Guhlich-Bornhof U, Ge B, Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SH
RgGuinier 3.1 nm
Dmax 12.7 nm
VolumePorod 110 nm3

SASDEQ9 – Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP)

UniProt ID: Q8N884 (1-522) Cyclic GMP-AMP synthase

UniProt ID: None (None-None) 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine
Cyclic GMP-AMP synthase2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine experimental SAS data
Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP) Rg histogram
Sample: Cyclic GMP-AMP synthase dimer, 123 kDa Homo sapiens protein
2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine dimer, 1 kDa
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 25
cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity. EMBO Rep (2019)
Liu H, Moura-Alves P, Pei G, Mollenkopf HJ, Hurwitz R, Wu X, Wang F, Liu S, Ma M, Fei Y, Zhu C, Koehler AB, Oberbeck-Mueller D, Hahnke K, Klemm M, Guhlich-Bornhof U, Ge B, Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SH
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 127 nm3

SASDET9 – Gliding motility protein MglB: A GAP of Myxococcus xanthus MglA

UniProt ID: Q1DB03 (1-159) Gliding motility protein MglB
Gliding motility protein MglB experimental SAS data
GASBOR model
Sample: Gliding motility protein MglB dimer, 34 kDa Myxococcus xanthus protein
Buffer: 150 mM NaCl, 1 mM DTT, 20 mM Tris-HCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 9
MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus. Nat Commun 10(1):5300 (2019)
Galicia C, Lhospice S, Varela PF, Trapani S, Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J
RgGuinier 2.8 nm
Dmax 10.3 nm
VolumePorod 56 nm3

SASDEU9 – Legionella pneumophila Phosphocholinase AnkX

UniProt ID: Q5ZXN6 (None-None) Phosphocholinase AnkX
Phosphocholinase AnkX experimental SAS data
GASBOR model
Sample: Phosphocholinase AnkX monomer, 107 kDa Legionella pneumophila protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Oct 20
Legionella pneumophila effector AnkX
Wenhua Zhang
RgGuinier 4.3 nm
Dmax 14.7 nm
VolumePorod 145 nm3

SASDEV9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant

UniProt ID: Q99418 (58-400) Cytohesin-2; ARNO truncation mutant
Cytohesin-2; ARNO truncation mutant experimental SAS data
DAMMIN model
Sample: Cytohesin-2; ARNO truncation mutant monomer, 40 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 25
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes. Structure (2019)
Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
RgGuinier 2.7 nm
Dmax 9.9 nm
VolumePorod 63 nm3

SASDEW9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO

UniProt ID: Q99418 (1-400) Cytohesin-2 ARF nucleotide-binding site opener
Cytohesin-2 ARF nucleotide-binding site opener experimental SAS data
GASBOR model
Sample: Cytohesin-2 ARF nucleotide-binding site opener dimer, 93 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 23
Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes. Structure (2019)
Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
RgGuinier 4.8 nm
Dmax 19.7 nm
VolumePorod 145 nm3

SASDEX9 – Class II pyruvate aldolase (HpcH/HpaI aldolase), wild-type hexamer in HEPES isolated using SEC-SAXS

UniProt ID: None (None-None) HpcH/HpaI aldolase
HpcH/HpaI aldolase experimental SAS data
GASBOR model
Sample: HpcH/HpaI aldolase hexamer, 165 kDa Rhizorhabdus wittichii RW1 protein
Buffer: 20 mM HEPES,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
EFAMIX , a tool to decompose inline chromatography SAXS data from partially overlapping components Protein Science (2021)
Konarev P, Graewert M, Jeffries C, Fukuda M, Cheremnykh T, Volkov V, Svergun D
RgGuinier 3.3 nm
Dmax 9.4 nm
VolumePorod 233 nm3

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

UniProt ID: P01241 (27-217) somapacitan

UniProt ID: P02768 (25-609) FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A

UniProt ID: P61768 (21-119) neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag

UniProt ID: P55899 (24-297) neonatal Fc receptor ectodomain alpha-chain
somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: Somapacitan monomer, 22 kDa Homo sapiens protein
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3