Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: P24300

SASDAK6 – Glucose Isomerase

UniProt ID: P24300 (None-None) Xylose isomerase

Sample:	Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer:	PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 24

Standard proteins
Erica Valentini

R_g^Guinier	3.4	nm
D_max	9.7	nm
Volume^Porod	293	nm³

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

UniProt ID: P24300 (1-388) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE

R_g^Guinier	3.3	nm
D_max	9.2	nm
Volume^Porod	229	nm³

SASDCJ8 – Glucose isomerase (WAXS)

UniProt ID: P24300 (None-None) Xylose isomerase

Sample:	Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer:	100 mM tris pH 8.0, 100 mM NaCl, 1 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 17

WAXS benchmark on standard proteins
Maxim Petoukhov

R_g^Guinier

3.2

SASDCR8 – Glucose isomerase

UniProt ID: P24300 (None-None) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	100 mM HEPES, 1 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21

A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet

R_g^Guinier

2.7

SASDPR4 – Consensus SAXS Profile - Xylose Isomerase

UniProt ID: P24300 (1-388) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	ConsensusBuffer_50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Hammel M, Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekiguchi H, Svergun D, Teixeira S, Thureau A, Weiss T, Whitten A, Wood K, Zuo X

R_g^Guinier	3.3	nm
D_max	10.1	nm
Volume^Porod	243	nm³

SASDPY4 – Consensus SANS Profile - Xylose isomerase in 100% v/v D2O buffer

UniProt ID: P24300 (1-388) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.5	nm

SASDP55 – Consensus SANS Profile - Xylose isomerase in H2O buffer

UniProt ID: P24300 (1-388) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.3	nm
D_max	9.7	nm

SASDP47 – Glucose Isomerase at a neutral pH and 5 MPa (1 atm) of pressure

UniProt ID: P24300 (1-380) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	25 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	3.4	nm
D_max	10.6	nm
Volume^Porod	253	nm³

SASDP57 – Glucose Isomerase at a neutral pH and 100 MPa (1000 atm) of pressure

UniProt ID: P24300 (1-380) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	25 mM HEPES, 150 mM NaCl, and 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	3.5	nm
D_max	10.6	nm
Volume^Porod	267	nm³

SASDPK7 – Xylose isomerase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

UniProt ID: P24300 (1-388) Xylose isomerase

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	3.2	nm
D_max	14.1	nm
Volume^Porod	228	nm³

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