Browse by DISSEMINATION: Published

SASDVW5 – alpha-amylase AMY3

Alpha-amylase 3, chloroplastic experimental SAS data
BILBOMD model
Sample: Alpha-amylase 3, chloroplastic dimer, 187 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 100 mM NaCl, 0.2 mM TCEP, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 May 16
The Pseudoenzyme β‐Amylase9 From Arabidopsis Activates α‐Amylase3: A Possible Mechanism to Promote Stress‐Induced Starch Degradation Proteins: Structure, Function, and Bioinformatics (2025)
Berndsen C, Storm A, Sardelli A, Hossain S, Clermont K, McFather L, Connor M, Monroe J
RgGuinier 5.1 nm
Dmax 21.5 nm
VolumePorod 444 nm3

SASDVX5 – pseudoamylase BAM9

Inactive beta-amylase 9 experimental SAS data
ALPHAFOLD model
Sample: Inactive beta-amylase 9 monomer, 50 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 100 mM NaCl, 0.2 mM TCEP, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 May 16
The Pseudoenzyme β‐Amylase9 From Arabidopsis Activates α‐Amylase3: A Possible Mechanism to Promote Stress‐Induced Starch Degradation Proteins: Structure, Function, and Bioinformatics (2025)
Berndsen C, Storm A, Sardelli A, Hossain S, Clermont K, McFather L, Connor M, Monroe J
RgGuinier 2.4 nm
Dmax 8.7 nm
VolumePorod 87 nm3

SASDVY5 – pseudoamylase BAM9 bound to alpha-amylase AMY3

Inactive beta-amylase 9Alpha-amylase 3, chloroplastic experimental SAS data
BILBOMD model
Sample: Inactive beta-amylase 9 monomer, 50 kDa Arabidopsis thaliana protein
Alpha-amylase 3, chloroplastic monomer, 94 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 100 mM NaCl, 0.2 mM TCEP, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 May 16
The Pseudoenzyme β‐Amylase9 From Arabidopsis Activates α‐Amylase3: A Possible Mechanism to Promote Stress‐Induced Starch Degradation Proteins: Structure, Function, and Bioinformatics (2025)
Berndsen C, Storm A, Sardelli A, Hossain S, Clermont K, McFather L, Connor M, Monroe J
RgGuinier 5.0 nm
Dmax 25.5 nm
VolumePorod 380 nm3

SASDU85 – Wild-type phosphoserine phosphatase RsbU dimer

Phosphoserine phosphatase RsbU experimental SAS data
MULTIFOXS model
Sample: Phosphoserine phosphatase RsbU dimer, 77 kDa Bacillus subtilis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 24
A General Mechanism for Initiating the General Stress Response in Bacteria. bioRxiv (2025)
Baral R, Ho K, Kumar RP, Hopkins JB, Watkins MB, LaRussa S, Caban-Penix S, Calderone LA, Bradshaw N
RgGuinier 3.9 nm
Dmax 14.3 nm
VolumePorod 117 nm3

SASDU95 – Hyperactive variant of phosphoserine phosphatase RsbU (Q94L) bound to the activator serine/threonine-protein kinase RsbT (heterotetrameric complex)

Serine/threonine-protein kinase RsbTPhosphoserine phosphatase RsbU (Q94L) experimental SAS data
Serine/threonine-protein kinase RsbT Phosphoserine phosphatase RsbU (Q94L) Kratky plot
Sample: Serine/threonine-protein kinase RsbT dimer, 29 kDa Bacillus subtilis (strain … protein
Phosphoserine phosphatase RsbU (Q94L) dimer, 77 kDa Bacillus subtilis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 24
A General Mechanism for Initiating the General Stress Response in Bacteria. bioRxiv (2025)
Baral R, Ho K, Kumar RP, Hopkins JB, Watkins MB, LaRussa S, Caban-Penix S, Calderone LA, Bradshaw N
RgGuinier 3.5 nm
Dmax 12.3 nm
VolumePorod 119 nm3

SASDVY6 – Wild-type full-length Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4)

Mitochondrial intermembrane space import and assembly protein 40 experimental SAS data
Mitochondrial intermembrane space import and assembly protein 40 Kratky plot
Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26
NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism. EMBO J (2025)
Brosey CA, Shen R, Tainer JA
RgGuinier 2.9 nm
Dmax 9.2 nm
VolumePorod 56 nm3

SASDVZ6 – Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) triple mutant I12A/F14A/H20A (AIA-A)

Mitochondrial intermembrane space import and assembly protein 40 experimental SAS data
Mitochondrial intermembrane space import and assembly protein 40 Kratky plot
Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26
NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism. EMBO J (2025)
Brosey CA, Shen R, Tainer JA
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 62 nm3

SASDV27 – Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) point mutant H20D

Mitochondrial intermembrane space import and assembly protein 40 experimental SAS data
Mitochondrial intermembrane space import and assembly protein 40 Kratky plot
Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26
NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism. EMBO J (2025)
Brosey CA, Shen R, Tainer JA
RgGuinier 3.2 nm
Dmax 11.2 nm
VolumePorod 66 nm3

SASDV37 – Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) point mutant L28D

Mitochondrial intermembrane space import and assembly protein 40 experimental SAS data
Mitochondrial intermembrane space import and assembly protein 40 Kratky plot
Sample: Mitochondrial intermembrane space import and assembly protein 40 monomer, 16 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 26
NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism. EMBO J (2025)
Brosey CA, Shen R, Tainer JA
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 52 nm3

SASDV47 – Peptide-linked fusion protein of Apoptosis-inducing factor 1 AIF(104-613) point mutant W196A and the N-terminal segment of Mitochondrial intermembrane space import and assembly protein 40 CHCHD4(1-45)

Mitochondrial intermembrane space import and assembly protein 40Apoptosis-inducing factor 1, mitochondrial experimental SAS data
Mitochondrial intermembrane space import and assembly protein 40 Apoptosis-inducing factor 1, mitochondrial Kratky plot
Sample: Mitochondrial intermembrane space import and assembly protein 40 dimer, 12 kDa Homo sapiens protein
Apoptosis-inducing factor 1, mitochondrial dimer, 113 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 28
NADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism. EMBO J (2025)
Brosey CA, Shen R, Tainer JA
RgGuinier 3.9 nm
Dmax 12.9 nm
VolumePorod 289 nm3