Browse by DISSEMINATION: Published

SASDX39 – Group IIC Intron Domain 1

Group IIC Intron Domain 1 experimental SAS data
DAMMIF model
Sample: Group IIC Intron Domain 1 monomer, 89 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Jul 7
Dynamic assembly of a large multidomain ribozyme visualized by cryo-electron microscopy. Nat Commun 16(1):10195 (2025)
Jadhav S, Maiorca M, Manigrasso J, Saha S, Rakitch A, Muscat S, Mulvaney T, De Vivo M, Topf M, Marcia M
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 188 nm3

SASDX49 – Group IIC intron Domain 1, 2 & 3

Group IIC Intron Domain 1, 2 & 3 experimental SAS data
DAMMIF model
Sample: Group IIC Intron Domain 1, 2 & 3 monomer, 103 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 2
Dynamic assembly of a large multidomain ribozyme visualized by cryo-electron microscopy. Nat Commun 16(1):10195 (2025)
Jadhav S, Maiorca M, Manigrasso J, Saha S, Rakitch A, Muscat S, Mulvaney T, De Vivo M, Topf M, Marcia M
RgGuinier 3.6 nm
Dmax 12.0 nm
VolumePorod 195 nm3

SASDX59 – Group IIC intron Domain 1 & 2

Group IIC Intron Domain 1 & 2 experimental SAS data
DAMMIF model
Sample: Group IIC Intron Domain 1 & 2 monomer, 96 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 2
Dynamic assembly of a large multidomain ribozyme visualized by cryo-electron microscopy. Nat Commun 16(1):10195 (2025)
Jadhav S, Maiorca M, Manigrasso J, Saha S, Rakitch A, Muscat S, Mulvaney T, De Vivo M, Topf M, Marcia M
RgGuinier 3.6 nm
Dmax 11.0 nm
VolumePorod 179 nm3

SASDX69 – Group IIC intron Domain 1, 2. 3 & 4

Group IIC intron Domain 1, 2, 3 & 4 experimental SAS data
DAMMIF model
Sample: Group IIC intron Domain 1, 2, 3 & 4 monomer, 125 kDa Oceanobacillus iheyensis RNA
Buffer: 10 mM MgCl2, 5 mM Na-MES, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 2
Dynamic assembly of a large multidomain ribozyme visualized by cryo-electron microscopy. Nat Commun 16(1):10195 (2025)
Jadhav S, Maiorca M, Manigrasso J, Saha S, Rakitch A, Muscat S, Mulvaney T, De Vivo M, Topf M, Marcia M
RgGuinier 4.2 nm
Dmax 12.6 nm
VolumePorod 247 nm3

SASDWH4 – Class V GTP aptamer

Class V GTP aptamer apo experimental SAS data
Class V GTP aptamer apo Kratky plot
Sample: Class V GTP aptamer apo, 22 kDa Escherichia coli RNA
Buffer: 50 mM Bis-Tris, 50 mM KCl, 2 mM MgCl2, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Jul 19
Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex. Nucleic Acids Res 53(22) (2025)
Stafflinger H, Neißner K, Bartsch S, Pichler AK, Bartosik K, Dhamotharan K, Abele R, Duchardt-Ferner E, Micura R, Schindelin H, Wöhnert J
RgGuinier 3.3 nm
Dmax 15.3 nm
VolumePorod 99 nm3

SASDWJ4 – Class V GTP aptamer complex with GTP

Class V GTP aptamerGuanosine triphosphate experimental SAS data
COOT model
Sample: Class V GTP aptamer monomer, 22 kDa Escherichia coli RNA
Guanosine triphosphate monomer, 1 kDa
Buffer: 50 mM Bis-Tris, 50 mM KCl, 2 mM MgCl2, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Jul 19
Crystal structure of the class V GTP-binding RNA aptamer bound to its ligand: GTP recognition by a topologically complex intermolecular G-quadruplex. Nucleic Acids Res 53(22) (2025)
Stafflinger H, Neißner K, Bartsch S, Pichler AK, Bartosik K, Dhamotharan K, Abele R, Duchardt-Ferner E, Micura R, Schindelin H, Wöhnert J
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 34 nm3

SASDWC7 – SEC-SAXS data of wildtype human apo estrogen receptor alpha ligand binding domain (LBD) at pH 7.5

Estrogen receptor alpha experimental SAS data
DAMMIF model
Sample: Estrogen receptor alpha dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5% glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Nov 9
A ternary switch model governing ERα ligand binding domain conformation. Nat Commun 16(1):10363 (2025)
McDougal DP, Pederick JL, Novick SJ, Jovcevski B, Warrender AK, Pascal BD, Griffin PR, Bruning JB
RgGuinier 2.4 nm
Dmax 7.4 nm
VolumePorod 74 nm3

SASDWD7 – SEC-SAXS data of wildtype Melanotaenia fluviatilis apo estrogen receptor alpha ligand binding domain (LBD) at pH 7.5

Estrogen receptor alpha experimental SAS data
DAMMIF model
Sample: Estrogen receptor alpha dimer, 57 kDa Melanotaenia fluviatilis protein
Buffer: 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5% glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioSAXS, Australian Synchrotron on 2024 Nov 9
A ternary switch model governing ERα ligand binding domain conformation. Nat Commun 16(1):10363 (2025)
McDougal DP, Pederick JL, Novick SJ, Jovcevski B, Warrender AK, Pascal BD, Griffin PR, Bruning JB
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 72 nm3

SASDXK2 – Enabled/vasodilator-stimulated phosphoprotein homology 1 domain (EVH1) from Mus musculus Homer1

EVH1 domain of Homer protein homolog 1 from mouse experimental SAS data
XPLOR-NIH model
Sample: EVH1 domain of Homer protein homolog 1 from mouse monomer, 14 kDa Mus musculus protein
Buffer: 20mM NaCl, 50 mM NaPi, 0.02% of sodium-azide, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Nov 9
Structural Modeling and Dynamics of the Full-Length Homer1 Multimer. Proteins (2025)
Kálmán ZE, Czajlik A, Maruzs B, Farkas F, Pap I, Homonnay C, Klumpler T, Batta G, Gáspári Z, Péterfia B
RgGuinier 1.6 nm
Dmax 5.3 nm
VolumePorod 23 nm3

SASDWT5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) SEC-SAXS

Breast cancer type 2 susceptibility protein (BRC repeat 4)DNA repair protein RAD51 homolog 1 (Δ97) experimental SAS data
ALPHAFOLD model
Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein
DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2
Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination (2025)
Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 57 nm3