Browse by MODEL: Ab initio only

SASDQ95 – The complex formed between E. coli S-adenosylmethionine synthase and wild-type S-adenosyl-L-methionine lyase from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jun 17
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.5 nm
VolumePorod 325 nm3

SASDQA5 – The complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A) experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase (E68Q/Q94A) tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 May 7
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 326 nm3

SASDSC5 – dark-state long-linker LOV-activated diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data
GASBOR model
Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 2.9 nm
Dmax 11.1 nm
VolumePorod 115 nm3

SASDN68 – Pikachurin C-terminal LG3 domain (SEC-SAXS)

Pikachurin experimental SAS data
GASBOR model
Sample: Pikachurin monomer, 22 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179 Science Signaling 16(795) (2023)
Patil D, Pantalone S, Cao Y, Laboute T, Novick S, Singh S, Savino S, Faravelli S, Magnani F, Griffin P, Singh A, Forneris F, Martemyanov K
RgGuinier 2.2 nm
Dmax 8.2 nm
VolumePorod 48 nm3

SASDN78 – Pikachurin N-terminal FnIII(1-2) fragment (SEC-SAXS)

Pikachurin N-terminal FnIII(1-2) domains experimental SAS data
GASBOR model
Sample: Pikachurin N-terminal FnIII(1-2) domains monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179 Science Signaling 16(795) (2023)
Patil D, Pantalone S, Cao Y, Laboute T, Novick S, Singh S, Savino S, Faravelli S, Magnani F, Griffin P, Singh A, Forneris F, Martemyanov K
RgGuinier 3.4 nm
Dmax 16.4 nm
VolumePorod 44 nm3

SASDN88 – Pikachurin C-terminal EGF-LG3 fragment (SEC-SAXS)

Pikachurin experimental SAS data
GASBOR model
Sample: Pikachurin monomer, 26 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179 Science Signaling 16(795) (2023)
Patil D, Pantalone S, Cao Y, Laboute T, Novick S, Singh S, Savino S, Faravelli S, Magnani F, Griffin P, Singh A, Forneris F, Martemyanov K
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 52 nm3

SASDJ26 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-monomer

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial monomer, 70 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam
RgGuinier 3.8 nm
Dmax 13.9 nm
VolumePorod 160 nm3

SASDJ36 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer

dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: Dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam
RgGuinier 5.0 nm
Dmax 18.2 nm
VolumePorod 516 nm3

SASDFY9 – Sperm-associated antigen 1 (SPAG1)

Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 7.3 nm
Dmax 34.0 nm
VolumePorod 374 nm3

SASDFZ9 – RuvB-like 1 and 2, sperm-associated antigen 1 complex (His-RuvBL1/RuvBL2/SPAG1)

RuvB-like 1RuvB-like 2Sperm-associated antigen 1 experimental SAS data
DAMMIF model
Sample: RuvB-like 1 hexamer, 311 kDa Homo sapiens protein
RuvB-like 2 hexamer, 311 kDa Homo sapiens protein
Sperm-associated antigen 1 monomer, 104 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21
R2SP
Raphael Dos Santos Morais
RgGuinier 6.8 nm
Dmax 34.8 nm
VolumePorod 1800 nm3