Browse by MODEL: Hybrid

SASDLX5 – Wild-type human mitochondrial deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial experimental SAS data
BUNCH model
Sample: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial trimer, 80 kDa Homo sapiens protein
Buffer: 20 mM citrate buffer, 1 mM DTT, 0.1 mM PMSF and 1 mM MgCl2, pH: 5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Feb 1
Molecular shape and prominent role of β-strand swapping in organization of dUTPase oligomers FEBS Letters 583(5):865-871 (2009)
Takács E, Barabás O, Petoukhov M, Svergun D, Vértessy B
RgGuinier 2.6 nm
Dmax 7.0 nm
VolumePorod 101 nm3

SASDLY5 – Monomer-dimer equilibrium of glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glutamate--tRNA ligase dimer, 108 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 35 mM HEPES⁄NaOH, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 19
Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl-tRNA synthetase and determination of its oligomeric structure in solution FEBS Journal 276(5):1398-1417 (2009)
Paravisi S, Fumagalli G, Riva M, Morandi P, Morosi R, Konarev P, Petoukhov M, Bernier S, Chênevert R, Svergun D, Curti B, Vanoni M
RgGuinier 3.5 nm
Dmax 11.0 nm
VolumePorod 123 nm3

SASDA94 – Der p21

Der p21, allegren experimental SAS data
DAMMIN model
Sample: Der p21, allegren dimer, 26 kDa Escherichia coli protein
Buffer: 10 mM Hepes , pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 24
Characterization of Der p 21, a new important allergen derived from the gut of house dust mites. Allergy 63(6):758-67 (2008)
Weghofer M, Dall'Antonia Y, Grote M, Stöcklinger A, Kneidinger M, Balic N, Krauth MT, Fernández-Caldas E, Thomas WR, van Hage M, Vieths S, Spitzauer S, Horak F, Svergun DI, Konarev PV, Valent P, Thalhamer J, Keller W, Valenta R, Vrtala S
RgGuinier 2.7 nm
Dmax 8.5 nm
VolumePorod 50 nm3

SASDAN5 – Cross-linked complex CytC_Adr

Cytochrome CAdrenodoxin experimental SAS data
DAMMIN model
Sample: Cytochrome C monomer, 11 kDa Escherichia coli protein
Adrenodoxin monomer, 11 kDa Escherichia coli protein
Buffer: 20 mM HEPES 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jun 15
Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy. J Am Chem Soc 130(20):6395-403 (2008)
Xu X, Reinle W, Hannemann F, Konarev PV, Svergun DI, Bernhardt R, Ubbink M
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 42 nm3

SASDAP5 – Native complex CytC_Adr

Cytochrome C dimerAdrenodoxin dimer experimental SAS data
DAMMIN model
Sample: Cytochrome C dimer dimer, 22 kDa Escherichia coli protein
Adrenodoxin dimer dimer, 22 kDa Escherichia coli protein
Buffer: 20 mM HEPES 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jun 15
Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy. J Am Chem Soc 130(20):6395-403 (2008)
Xu X, Reinle W, Hannemann F, Konarev PV, Svergun DI, Bernhardt R, Ubbink M
RgGuinier 2.9 nm
Dmax 9.5 nm
VolumePorod 64 nm3

SASDLZ6 – Hexamer - monomer equilibrium of Glutamate Synthase

Glutamate synthase [NADPH] large chainGlutamate synthase [NADPH] small chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glutamate synthase [NADPH] large chain hexamer, 968 kDa Azospirillum brasilense protein
Glutamate synthase [NADPH] small chain hexamer, 296 kDa Azospirillum brasilense protein
Buffer: 25 mM Hepes/KOH, 1 mM EDTA, 1 mM dithiothreitol, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 19
The Subnanometer Resolution Structure of the Glutamate Synthase 1.2-MDa Hexamer by Cryoelectron Microscopy and Its Oligomerization Behavior in Solution Journal of Biological Chemistry 283(13):8237-8249 (2008)
Cottevieille M, Larquet E, Jonic S, Petoukhov M, Caprini G, Paravisi S, Svergun D, Vanoni M, Boisset N
RgGuinier 7.7 nm
Dmax 22.4 nm

SASDMS4 – Listeria monocytogenes invasion protein internalin B

Internalin B experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Internalin B monomer, 36 kDa Listeria monocytogenes serotype … protein
Buffer: 25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Nov 11
X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB Journal of Molecular Biology 377(2):489-500 (2008)
Niemann H, Petoukhov M, Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 54 nm3

SASDMT4 – Complex of Met receptor with InlB

Hepatocyte growth factor receptorInternalin B experimental SAS data
SASREF CV model
Sample: Hepatocyte growth factor receptor monomer, 100 kDa Mus musculus protein
Internalin B monomer, 36 kDa Listeria monocytogenes serotype … protein
Buffer: 25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Mar 1
X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB Journal of Molecular Biology 377(2):489-500 (2008)
Niemann H, Petoukhov M, Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 270 nm3

SASDNW2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268)

Fab fragment in complex with small molecule hapten, crystal form-1 experimental SAS data
DAMMIN model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 25
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 71 nm3

SASDNX2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) bound to TSC ligand

Fab fragment in complex with small molecule hapten, crystal form-1(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL experimental SAS data
DAMMIN model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL monomer, 0 kDa
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 27
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 4.1 nm
Dmax 16.0 nm
VolumePorod 139 nm3