MACROMOLECULE TYPE: heterocomplex

SASDCA3 – Zinc finger and BTB domain-containing protein 38 (ZBTB38) complexed with methylated C-terminal ZBTB38 binding sequence

Zinc finger and BTB domain-containing protein 38methylated C-terminal ZBTB38 binding sequence experimental SAS data
MONSA model
Sample: Zinc finger and BTB domain-containing protein 38 monomer, 17 kDa Homo sapiens protein
Methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Nov 11
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
Pozner A, Hudson NO, Trewhella J, Terooatea TW, Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 50 nm3

SASDB79 – Basic domain of human telomeric repeat-binding factor 2 (TRF2) in complex with telomeric DNA duplex

Basic domain of telomeric repeat-binding factor 2telomere DNA duplex experimental SAS data
MONSA model
Sample: Basic domain of telomeric repeat-binding factor 2 monomer, 5 kDa Homo sapiens protein
Telomere DNA duplex monomer, 11 kDa DNA
Buffer: 20 mM Tris-HCl, 50 mM LiCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 May 3
Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
Necasová I, Janoušková E, Klumpler T, Hofr C
RgGuinier 1.7 nm
Dmax 6.0 nm
VolumePorod 20 nm3

SASDCM5 – Vaccinia virus binary E9 polymerase / DNA complex

DNA polymerase E9 exo- mutantDNA oligomer template-primer hairpin experimental SAS data
PYMOL model
Sample: DNA polymerase E9 exo- mutant monomer, 117 kDa Vaccinia virus protein
DNA oligomer template-primer hairpin monomer, 9 kDa synthetic construct DNA
Buffer: 20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 12
The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Nat Commun 8(1):1455 (2017)
Tarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 210 nm3

SASDBJ2 – Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.

40bp long dsDNA-Sa OligonucleotideWild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). experimental SAS data
DAMMIN model
Sample: 40bp long dsDNA-Sa Oligonucleotide monomer, 25 kDa DNA
Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer: 0.5 x Tris/Borate/EDTA (TBE), pH:
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
RgGuinier 3.0 nm
Dmax 11.8 nm

SASDCC3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA

Nucleolysin TIA-1 isoform p40poly U 15mer experimental SAS data
Nucleolysin TIA-1 isoform p40 poly U 15mer Kratky plot
Sample: Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Poly U 15mer monomer, 5 kDa RNA
Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28
Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins. Angew Chem Int Ed Engl 56(32):9322-9325 (2017)
Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M
RgGuinier 2.4 nm
Dmax 8.7 nm
VolumePorod 39 nm3

SASDCE3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)

poly U 15merNucleolysin TIA-1 isoform p40 experimental SAS data
poly U 15mer Nucleolysin TIA-1 isoform p40 Kratky plot
Sample: Poly U 15mer monomer, 5 kDa RNA
Nucleolysin TIA-1 isoform p40 monomer, 31 kDa Homo sapiens protein
Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2015 Dec 1
Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins. Angew Chem Int Ed Engl 56(32):9322-9325 (2017)
Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M
RgGuinier 2.4 nm
Dmax 8.6 nm
VolumePorod 40 nm3

SASDCH3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPATG containing construct for sortase mediated protein ligation)

poly U 15merNucleolysin TIA-1 isoform p40 experimental SAS data
poly U 15mer Nucleolysin TIA-1 isoform p40 Kratky plot
Sample: Poly U 15mer monomer, 5 kDa RNA
Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Buffer: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 May 4
Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins. Angew Chem Int Ed Engl 56(32):9322-9325 (2017)
Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M
RgGuinier 2.4 nm
Dmax 8.6 nm
VolumePorod 40 nm3

SASDB48 – Major viral transcription factor ICP4/Immediate Early 3 DNA (ICP4N IE3_19mer) complex

Major viral transcription factor ICP4Immediate Early 3 experimental SAS data
NONE model
Sample: Major viral transcription factor ICP4 dimer, 49 kDa Human alphaherpesvirus 1 protein
Immediate Early 3 dimer, 12 kDa DNA
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Jul 27
The herpes viral transcription factor ICP4 forms a novel DNA recognition complex. Nucleic Acids Res 45(13):8064-8078 (2017)
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
RgGuinier 2.5 nm
Dmax 8.3 nm
VolumePorod 90 nm3

SASDBN6 – Nucleolysin TIA-1 isoform p40 in complex with ACUCCUUUUU RNA

Nucleolysin TIA-1 isoform p40RNA (ACUCCUUUUU) experimental SAS data
Nucleolysin TIA-1 isoform p40 RNA (ACUCCUUUUU) Kratky plot
Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
RNA (ACUCCUUUUU) monomer, 1 kDa RNA
Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27
TIA-1 RRM23 binding and recognition of target oligonucleotides. Nucleic Acids Res 45(8):4944-4957 (2017)
Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA
RgGuinier 2.2 nm
Dmax 6.6 nm
VolumePorod 30 nm3

SASDBP6 – Nucleolysin TIA-1 isoform p40 in complex with ACTCCTTTTT DNA

Nucleolysin TIA-1 isoform p40DNA (ACTCCTTTTT) experimental SAS data
Nucleolysin TIA-1 isoform p40 DNA (ACTCCTTTTT) Kratky plot
Sample: Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
DNA (ACTCCTTTTT) monomer, 1 kDa DNA
Buffer: 20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27
TIA-1 RRM23 binding and recognition of target oligonucleotides. Nucleic Acids Res 45(8):4944-4957 (2017)
Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA
RgGuinier 2.2 nm
Dmax 6.7 nm
VolumePorod 32 nm3