Browse by MACROMOLECULE type: protein

SASDH24 – Calcium-Activated Chloride Channel Regulator 1 VWA domain

Calcium-activated chloride channel regulator 1 experimental SAS data
DAMFILT model
Sample: Calcium-activated chloride channel regulator 1 monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jun 6
Structural and Biophysical Analysis of the CLCA1 VWA Domain Suggests Mode of TMEM16A Engagement. Cell Rep 30(4):1141-1151.e3 (2020)
Berry KN, Brett TJ
RgGuinier 1.8 nm
Dmax 6.6 nm

SASDH34 – Calcium-Activated Chloride Channel Regulator 1 CAT-CYS-VWA domain construct

Calcium-activated chloride channel regulator 1 experimental SAS data
DAMFILT model
Sample: Calcium-activated chloride channel regulator 1 monomer, 52 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 6
Structural and Biophysical Analysis of the CLCA1 VWA Domain Suggests Mode of TMEM16A Engagement. Cell Rep 30(4):1141-1151.e3 (2020)
Berry KN, Brett TJ
RgGuinier 3.4 nm
Dmax 13.4 nm

SASDF87 – Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O

Binary toxin receptor Cqm1 protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Binary toxin receptor Cqm1 protein dimer, 129 kDa synthetic construct protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24
Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
Sharma M, Aswal V, Kumar V, Chidambaram R
RgGuinier 2.8 nm
Dmax 9.7 nm

SASDF97 – Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O

Binary toxin receptor Cqm1 proteinDeuterated BinB component of mosquito-larvicidal Binary toxin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Binary toxin receptor Cqm1 protein monomer, 65 kDa synthetic construct protein
Deuterated BinB component of mosquito-larvicidal Binary toxin monomer, 56 kDa synthetic construct protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 23
Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
Sharma M, Aswal V, Kumar V, Chidambaram R
RgGuinier 1.9 nm
Dmax 7.3 nm

SASDFA7 – Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O

Lysinibacillus Mosquito-larvicidal receptor binding component experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysinibacillus Mosquito-larvicidal receptor binding component monomer, 53 kDa synthetic construct protein
Buffer: 25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment: SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24
Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
Sharma M, Aswal V, Kumar V, Chidambaram R
RgGuinier 3.3 nm
Dmax 9.9 nm

SASDF85 – Pseudomonas aeruginosa antitoxin HigA: apo PaHigA

Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 21
Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 23 nm3

SASDEB9 – Flagellar brake protein YcgR from Escherichia coli

Flagellar brake protein YcgR experimental SAS data
SASREF model
Sample: Flagellar brake protein YcgR monomer, 29 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150mM NaCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jan 4
Structural insights into the mechanism of c-di-GMP-bound YcgR regulating flagellar motility in Escherichia coli. J Biol Chem 295(3):808-821 (2020)
Hou YJ, Yang WS, Hong Y, Zhang Y, Wang DC, Li DF
RgGuinier 2.6 nm
Dmax 9.1 nm
VolumePorod 44 nm3

SASDEC9 – Flagellar brake protein YcgR in complex with c-di-GMP from Escherichia coli

Flagellar brake protein YcgR in complex with c-di-GMP experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Flagellar brake protein YcgR in complex with c-di-GMP monomer, 29 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150mM NaCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jan 4
Structural insights into the mechanism of c-di-GMP-bound YcgR regulating flagellar motility in Escherichia coli. J Biol Chem 295(3):808-821 (2020)
Hou YJ, Yang WS, Hong Y, Zhang Y, Wang DC, Li DF
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 44 nm3

SASDHW3 – Binary-patterned 4-helix bundle de novo protein WA20

De novo protein WA20 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: De novo protein WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
Hyperstable De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm

SASDHX3 – Hyperstable de novo protein Super WA20 (SUWA)

Hyperstable de novo protein Super WA20 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Hyperstable de novo protein Super WA20 dimer, 25 kDa de novo protein protein
Buffer: 20 mM HEPES, 100 mM NaCl, 200 mM ArgHCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Feb 26
Hyperstable De Novo Protein with a Dimeric Bisecting Topology. ACS Synth Biol (2020)
Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R
RgGuinier 2.7 nm
Dmax 10.0 nm